基于活动的厌食症(ABA)模型:对急性期大脑神经炎症、氧化还原平衡和神经可塑性的影响。

IF 4.4 3区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Neurochemistry international Pub Date : 2024-09-06 DOI:10.1016/j.neuint.2024.105842
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引用次数: 0

摘要

一些证据表明,免疫炎症反应与神经性厌食症(AN)的发病机制有关。在此,我们研究了表现出厌食症样表型的大鼠大脑中炎症、氧化还原平衡和神经可塑性的关键介质可能发生的变化。我们使用基于活动的厌食症(ABA)范式模拟了青春期雌性大鼠的厌食症,并测量了前额叶皮层(PFC)和背侧海马(DH)中相关靶点的基因表达水平。我们观察到,在 ABA 动物的前额叶皮层和海马背侧,促炎细胞因子 IL-1β 和 TNF-α、炎性组 NLRP3 以及小胶质细胞标志物 CD11b 的 mRNA 水平均有所降低。相反,同时作为促炎和抗炎细胞因子的 IL-6 的 mRNA 则有所增加。此外,我们还观察到 PFC 中不同抗氧化酶的整体上调,而在 DH 中,除了 MT1α 外,其他抗氧化酶没有受到影响,或者相反。有趣的是,ABA动物在PFC和DH中都显示出神经可塑性标志物BDNF水平的升高。我们的数据表明,ABA诱导与大脑解剖特异性神经炎症介质、氧化平衡和神经可塑性的改变有关。尽管还需要进行更多的研究,但这些结果为这些系统在复杂的AN发病机制中的作用提供了重要信息。
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Activity-based anorexia (ABA) model: Effects on brain neuroinflammation, redox balance and neuroplasticity during the acute phase

Several evidences suggest that immuno-inflammatory responses are involved in the pathogenesis of anorexia nervosa (AN). Herein we investigate the possible alteration of key mediators of inflammation, redox balance, and neuroplasticity in the brain of rats showing an anorexic-like phenotype. We modeled AN in adolescent female rats using the activity-based anorexia (ABA) paradigm and measured gene expression levels of targets of interest in the prefrontal cortex (PFC) and dorsal hippocampus (DH). We observed reduced mRNA levels of pro-inflammatory cytokines IL-1β and TNF-α, the inflammasome NLRP3, and the microglial marker CD11b in both PFC and DH of ABA animals. Conversely, the mRNA of IL-6, which acts as both a pro-inflammatory and anti-inflammatory cytokine, was increased. Moreover, we observed an overall upregulation of different antioxidant enzymes in PFC, while their profile was not affected or opposite in the DH, with the exception of MT1α. Interestingly, ABA animals showed elevated levels of the neuroplasticity marker BDNF in both PFC and DH. Our data indicate that ABA induction is associated with anatomical-specific cerebral alteration of mediators of neuroinflammation, oxidative balance and neuroplasticity. Although more research should be conducted, these results add important information about the role of these systems in the complex AN etiopathogenesis.

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来源期刊
Neurochemistry international
Neurochemistry international 医学-神经科学
CiteScore
8.40
自引率
2.40%
发文量
128
审稿时长
37 days
期刊介绍: Neurochemistry International is devoted to the rapid publication of outstanding original articles and timely reviews in neurochemistry. Manuscripts on a broad range of topics will be considered, including molecular and cellular neurochemistry, neuropharmacology and genetic aspects of CNS function, neuroimmunology, metabolism as well as the neurochemistry of neurological and psychiatric disorders of the CNS.
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