鉴定非小细胞肺癌患者唾液中 WGA 识别的 N-连接聚糖。

IF 2.4 3区 化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Carbohydrate Research Pub Date : 2024-09-03 DOI:10.1016/j.carres.2024.109256
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引用次数: 0

摘要

非小细胞肺癌(NSCLC)是导致癌症相关死亡的主要原因之一。由于唾液诊断具有无创和材料丰富的特点,因此是临床应用的重要方法。本研究的目的是调查基于小麦胚芽凝集素(WGA)识别唾液蛋白N-连接聚糖图谱的差异,以区分非小细胞肺癌(NSCLC)患者和对照组。我们使用 WGA-磁性颗粒共轭物分离了健康志愿者(HV,n = 35)、良性肺病(BPD,n = 35)、肺腺癌(ADC,n = 35)和鳞状细胞癌(NSCLC,n = 35)患者唾液中的糖蛋白、随后,用 PNGase F 从分离出的蛋白质中释放出连接的聚糖,并分别用 MALDI-TOF/TOF-MS对释放出的聚糖进行鉴定和注释。结果显示,从HV、BPD、ADC和SCC的唾液样本中分别鉴定和注释了34、35、39和44个WGA识别的N-聚糖。此外,与 HV(71.9%)相比,WGA 在 BPD(81.2%)、ADC(90.1%)和 SCC(88.7%)中识别的 N-聚糖比例有所增加。WGA特异性识别的两个N-糖峰(m/z 2286.799和3399.211)仅出现在NSCLC中。这些研究结果表明,WGA 识别的唾液糖型改变,如含硅酸和 GlcNAc 的 N-聚糖,可作为诊断 NSCLC 患者的潜在个性化生物标记物。
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Identification of N-linked glycans recognized by WGA in saliva from patients with non-small cell lung cancer

Non-small cell lung cancer (NSCLC) is one of the leading causes of cancer-related deaths. Saliva diagnosis is an essential approach for clinical applications owing to its noninvasive and material-rich features. The purpose of this study was to investigate differences in wheat germ agglutinin (WGA)-based recognition of salivary protein N-linked glycan profiles to distinguish non-small cell lung cancer (NSCLC) patients from controls. We used WGA-magnetic particle conjugates to isolate glycoproteins in the pooled saliva of healthy volunteers (HV, n = 35), patients with benign pulmonary disease (BPD, n = 35), lung adenocarcinoma (ADC, n = 35), and squamous cell carcinoma (SCC, n = 35), following to release the N-linked glycans from the isolated proteins with PNGase F, and further identified and annotated the released glycans by MALDI-TOF/TOF-MS, respectively. The results showed that 34, 35, 39, and 44 N-glycans recognized by WGA were identified and annotated from pooled saliva samples of HV, BPD, ADC, and SCC, respectively. Furthermore, the proportion of N-glycans recognized by WGA in BPD (81.2 %), ADC (90.1 %), and SCC (88.7 %), increased compared to HV (71.9 %). Two N-glycan peaks (m/z 2286.799, and 3399.211) specifically recognized by WGA were present only in NSCLC. These findings suggest that altered salivary glycopatterns such as sialic acids and GlcNAc containing N-glycans recognized by WGA might serve as potential personalized biomarkers for the diagnosis of NSCLC patients.

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来源期刊
Carbohydrate Research
Carbohydrate Research 化学-生化与分子生物学
CiteScore
5.00
自引率
3.20%
发文量
183
审稿时长
3.6 weeks
期刊介绍: Carbohydrate Research publishes reports of original research in the following areas of carbohydrate science: action of enzymes, analytical chemistry, biochemistry (biosynthesis, degradation, structural and functional biochemistry, conformation, molecular recognition, enzyme mechanisms, carbohydrate-processing enzymes, including glycosidases and glycosyltransferases), chemical synthesis, isolation of natural products, physicochemical studies, reactions and their mechanisms, the study of structures and stereochemistry, and technological aspects. Papers on polysaccharides should have a "molecular" component; that is a paper on new or modified polysaccharides should include structural information and characterization in addition to the usual studies of rheological properties and the like. A paper on a new, naturally occurring polysaccharide should include structural information, defining monosaccharide components and linkage sequence. Papers devoted wholly or partly to X-ray crystallographic studies, or to computational aspects (molecular mechanics or molecular orbital calculations, simulations via molecular dynamics), will be considered if they meet certain criteria. For computational papers the requirements are that the methods used be specified in sufficient detail to permit replication of the results, and that the conclusions be shown to have relevance to experimental observations - the authors'' own data or data from the literature. Specific directions for the presentation of X-ray data are given below under Results and "discussion".
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