在疫苗开发过程中利用芽孢杆菌病毒表达的 BoHV-1 糖蛋白 D 增强免疫反应

IF 2.3 2区 农林科学 Q1 VETERINARY SCIENCES Veterinary journal Pub Date : 2024-09-06 DOI:10.1016/j.tvjl.2024.106228
Nguyen-Thanh Hoa , Haroon Afzal , Uudamsaikhan Gundegmaa , Odbileg Raadan , Li-Ting Cheng , Chun-Yen Chu , Thu-Dung Doan , Yao-Chi Chung
{"title":"在疫苗开发过程中利用芽孢杆菌病毒表达的 BoHV-1 糖蛋白 D 增强免疫反应","authors":"Nguyen-Thanh Hoa ,&nbsp;Haroon Afzal ,&nbsp;Uudamsaikhan Gundegmaa ,&nbsp;Odbileg Raadan ,&nbsp;Li-Ting Cheng ,&nbsp;Chun-Yen Chu ,&nbsp;Thu-Dung Doan ,&nbsp;Yao-Chi Chung","doi":"10.1016/j.tvjl.2024.106228","DOIUrl":null,"url":null,"abstract":"<div><p>Bovine herpesvirus 1 (BoHV-1), a significant pathogen in the alpha-herpesvirus subfamily, primarily infects cattle and causes the upper respiratory disease known as infectious bovine rhinotracheitis (IBR). In silico studies evaluated the BoHV-1 D protein to be non-allergenic, non-toxic, and highly antigenic, highlighting its potential as an antigen for vaccine development. Therefore, this study aimed to evaluate the efficacy of a subunit vaccine using the ectodomain of glycoprotein D (gD<sub>34–380</sub>) as an antigen. The truncated gD was successfully cloned and expressed in both <em>Escherichia coli</em> (<em>E. coli</em>, termed EgD) and baculovirus (termed BgD) systems, with expected molecular weights of 65 kDa and 50 kDa, respectively. For the vaccine formulation, the gD proteins were used either alone or in combination with in-house inactivated BoHV-1. Vaccination of mice and bovines showed that baculovirus-expressed gD<sub>34–380</sub> accelerated the antibody response. Moreover, the BgD-vaccinated group also showed significantly higher neutralizing antibody levels against BoHV-1 than the control group (p&lt;0.0001). In conclusion, our study found that BgD from BoHV-1 can increase the immune response and enhance vaccine efficacy.</p></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":null,"pages":null},"PeriodicalIF":2.3000,"publicationDate":"2024-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Enhanced immune response with baculovirus-expressed BoHV-1 glycoprotein D in vaccine development\",\"authors\":\"Nguyen-Thanh Hoa ,&nbsp;Haroon Afzal ,&nbsp;Uudamsaikhan Gundegmaa ,&nbsp;Odbileg Raadan ,&nbsp;Li-Ting Cheng ,&nbsp;Chun-Yen Chu ,&nbsp;Thu-Dung Doan ,&nbsp;Yao-Chi Chung\",\"doi\":\"10.1016/j.tvjl.2024.106228\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Bovine herpesvirus 1 (BoHV-1), a significant pathogen in the alpha-herpesvirus subfamily, primarily infects cattle and causes the upper respiratory disease known as infectious bovine rhinotracheitis (IBR). In silico studies evaluated the BoHV-1 D protein to be non-allergenic, non-toxic, and highly antigenic, highlighting its potential as an antigen for vaccine development. Therefore, this study aimed to evaluate the efficacy of a subunit vaccine using the ectodomain of glycoprotein D (gD<sub>34–380</sub>) as an antigen. The truncated gD was successfully cloned and expressed in both <em>Escherichia coli</em> (<em>E. coli</em>, termed EgD) and baculovirus (termed BgD) systems, with expected molecular weights of 65 kDa and 50 kDa, respectively. For the vaccine formulation, the gD proteins were used either alone or in combination with in-house inactivated BoHV-1. Vaccination of mice and bovines showed that baculovirus-expressed gD<sub>34–380</sub> accelerated the antibody response. Moreover, the BgD-vaccinated group also showed significantly higher neutralizing antibody levels against BoHV-1 than the control group (p&lt;0.0001). In conclusion, our study found that BgD from BoHV-1 can increase the immune response and enhance vaccine efficacy.</p></div>\",\"PeriodicalId\":23505,\"journal\":{\"name\":\"Veterinary journal\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.3000,\"publicationDate\":\"2024-09-06\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Veterinary journal\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1090023324001679\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"VETERINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Veterinary journal","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1090023324001679","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
引用次数: 0

摘要

牛疱疹病毒 1(BoHV-1)是α-疱疹病毒亚家族中的一种重要病原体,主要感染牛,并引起牛传染性鼻气管炎(IBR)等上呼吸道疾病。硅学研究评估认为,BoHV-1 的 D 蛋白无过敏性、无毒性且具有高度抗原性,突出了其作为疫苗开发抗原的潜力。因此,本研究旨在评估以糖蛋白 D 外显子域(gD34-380)为抗原的亚单位疫苗的功效。截短的 gD 被成功克隆并在大肠杆菌(E. coli,称为 EgD)和杆状病毒(Baculovirus,称为 BgD)系统中表达,预期分子量分别为 65kDa 和 50kDa。在疫苗配方中,gD 蛋白可单独使用,也可与内部灭活的 BoHV-1 结合使用。对小鼠和牛进行的疫苗接种表明,杆状病毒表达的 gD34-380 可加速抗体反应。此外,接种 BgD 疫苗组对 BoHV-1 的中和抗体水平也明显高于对照组(p
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Enhanced immune response with baculovirus-expressed BoHV-1 glycoprotein D in vaccine development

Bovine herpesvirus 1 (BoHV-1), a significant pathogen in the alpha-herpesvirus subfamily, primarily infects cattle and causes the upper respiratory disease known as infectious bovine rhinotracheitis (IBR). In silico studies evaluated the BoHV-1 D protein to be non-allergenic, non-toxic, and highly antigenic, highlighting its potential as an antigen for vaccine development. Therefore, this study aimed to evaluate the efficacy of a subunit vaccine using the ectodomain of glycoprotein D (gD34–380) as an antigen. The truncated gD was successfully cloned and expressed in both Escherichia coli (E. coli, termed EgD) and baculovirus (termed BgD) systems, with expected molecular weights of 65 kDa and 50 kDa, respectively. For the vaccine formulation, the gD proteins were used either alone or in combination with in-house inactivated BoHV-1. Vaccination of mice and bovines showed that baculovirus-expressed gD34–380 accelerated the antibody response. Moreover, the BgD-vaccinated group also showed significantly higher neutralizing antibody levels against BoHV-1 than the control group (p<0.0001). In conclusion, our study found that BgD from BoHV-1 can increase the immune response and enhance vaccine efficacy.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Veterinary journal
Veterinary journal 农林科学-兽医学
CiteScore
4.10
自引率
4.50%
发文量
79
审稿时长
40 days
期刊介绍: The Veterinary Journal (established 1875) publishes worldwide contributions on all aspects of veterinary science and its related subjects. It provides regular book reviews and a short communications section. The journal regularly commissions topical reviews and commentaries on features of major importance. Research areas include infectious diseases, applied biochemistry, parasitology, endocrinology, microbiology, immunology, pathology, pharmacology, physiology, molecular biology, immunogenetics, surgery, ophthalmology, dermatology and oncology.
期刊最新文献
Antitumor effects of inhibitors of ERK and Akt pathways in canine histiocytic sarcoma cell lines. Urinary Cystatin B as a marker of acute kidney injury in cats. Monoclonal antibody development and antigenic epitope identification of infectious bursal disease virus VP5 Use of respiratory signal analysis to assess severity of Brachycephalic Obstructive Airway Syndrome (BOAS) in dogs Metabolic profile of prepartum dairy cows and its influence on the immediate postpartum period, colostrum quality and passive immunity transference
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1