由 Pigmentiphaga sp. YJ18 中独特的 qut 簇介导的喹啉酸降解

IF 4.1 2区 环境科学与生态学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY International Biodeterioration & Biodegradation Pub Date : 2024-09-05 DOI:10.1016/j.ibiod.2024.105911
Kexin Wang , Yinhu Jiang , Ying Zhou, Jiale Ma, Qimiao Xu, Lu Xu, Jian He, Jiguo Qiu
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引用次数: 0

摘要

喹啉酸(QA)是生物体内通过犬尿氨酸途径产生的一种天然吡啶衍生物,因其神经毒性而与多种神经退行性疾病有关。虽然有报道称一些细菌能降解 QA,但其分子机制仍不完整。本研究在 Pigmentiphaga sp. YJ18 中发现了负责降解 QA 的独特 qut 簇。菌株 YJ18 能降解并利用 QA 作为生长的唯一碳源。菌株 YJ18 在 30 °C、pH 7.0 和 1%氯化钠条件下可在 48 小时内高效降解 100 mg/L QA。基因缺失结果表明,qutE 参与了 QA 降解的第一步,而 qutI 则介导 6-hydroxypicolinic acid(6HPA)向下游代谢物的转化。此外,QA 的降解还受到 MarR 家族转录调控因子 QutR 的负向调控。qut 基因簇由三个转录单元组成:qutABCDEF、qutGHIJKLMNO 和 qutR。电泳迁移测定结果表明,QutR 分别与 qutA、qutG 和 qutR 的启动子区域结合,共享部分 palindromic motif 5′-TCAG-N4-CTNN-3'。生物信息学分析表明,与 Pigmentiphaga 菌株共同进化的独特 qut 簇整合了 qui 簇和 pic 簇,它们分别负责将 QA 降解为 6HPA 和将 picolinic acid 降解为富马酸。总之,这项研究提供了一种新分离的菌株,它能够降解 QA,并具有独特的 qut 簇,同时还提供了关于细菌 QA 分解机制多样性的宝贵分子见解。
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Quinolinic acid degradation mediated by a unique qut cluster in Pigmentiphaga sp. YJ18

Quinolinic acid (QA), a natural pyridine derivative produced through the kynurenine pathway in living organisms, is linked to various neurodegenerative diseases due to its neurotoxicity. Although some bacteria have been reported to degrade QA, the underlying molecular mechanisms remain incomplete. In this study, a unique qut cluster responsible for QA degradation is identified in Pigmentiphaga sp. YJ18. The strain YJ18 could degrade and utilize QA as the sole carbon source for growth. Strain YJ18 efficiently degrade 100 mg/L QA within 48 h at 30 °C, pH 7.0, and 1% NaCl. The gene-disruption results showed that qutE is involved in the initial step of QA degradation, while qutI mediate the conversion of 6-hydroxypicolinic acid (6HPA) to downstream metabolites. Furthermore, the degradation of QA is negatively regulated by the MarR family transcriptional regulator QutR, which shared 61.97% amino similarity with PicR in Alcaligenes faecalis. The qut gene cluster consists of three transcriptional units: qutABCDEF, qutGHIJKLMNO, and qutR. Electrophoretic mobility shift assay results demonstrated that QutR binds to the promoter regions of the qutA, qutG, and qutR, respectively, sharing a partial palindromic motif 5′-TCAG-N4-CTNN-3’. Bioinformatics analysis reveals that the unique qut cluster, co-evolving with Pigmentiphaga strains, integrating the qui and pic clusters which are responsible for degradation of QA to 6HPA and picolinic acid to fumaric acid, respectively. Overall, this study provides a newly isolated strain capable of degrading QA with a unique qut cluster and valuable molecular insights into the diversity of QA catabolic mechanisms in bacteria.

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来源期刊
CiteScore
9.60
自引率
10.40%
发文量
107
审稿时长
21 days
期刊介绍: International Biodeterioration and Biodegradation publishes original research papers and reviews on the biological causes of deterioration or degradation.
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