{"title":"基于纳米分子平台的免疫传感器的制作，用于口腔癌的无创电化学检测：体外研究","authors":"Meenakshi Choudhary , Vinay Kumar , Ashik Babu Parambath , Shruti Caplash , Birendra Kumar Yadav , Satbir Kaur , Sapna Ratan Shah , Kavita Arora","doi":"10.1016/j.talo.2024.100352","DOIUrl":null,"url":null,"abstract":"<div><p>Silver nanoparticles (AgNPs)-Chitosan (Chi) functionalized cysteine (cys)-SAM (self-assembled monolayer) based electrochemical, label free, ultrasensitive, non-invasive immunosensing nanomolecular platform has been fabricated for the detection of novel OC biomarker S-100. This has been achieved through bio-functionalization of AgNPs-Chi/cys/mini gold slides by covalent immobilization of anti-S100 antibodies. Cyclic voltammetric (CV) studies establish successful fabrication of anti-S100/AgNPs-Chi/Au nanomolecular platform. Differential pulse voltammetric (DPV) measurements reveal that anti-S100/Chi-AgNPs/Au is able to sense 50 fg mL<sup>-1</sup> – 500 ng mL<sup>-1</sup> of S100 in buffer and in spiked saliva samples within 120 s interaction time having improved favorable shelf life and specificity. The fabricated platform is very selective and able to detect S100 in OC patient/s saliva samples warrants realization of simple non-invasive electrochemical immunosensor for <em>in situ</em> or <em>on-site</em> applications (especially for remote areas) for early diagnosis of OC while offering bio-compatibility at low cost.</p></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"10 ","pages":"Article 100352"},"PeriodicalIF":4.1000,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666831924000663/pdfft?md5=3345a9daaf0246157a50c3f630bbc45e&pid=1-s2.0-S2666831924000663-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Fabrication of nanomolecular platform based immunosensor for non-invasive electrochemical detection of oral cancer: An in vitro study\",\"authors\":\"Meenakshi Choudhary , Vinay Kumar , Ashik Babu Parambath , Shruti Caplash , Birendra Kumar Yadav , Satbir Kaur , Sapna Ratan Shah , Kavita Arora\",\"doi\":\"10.1016/j.talo.2024.100352\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Silver nanoparticles (AgNPs)-Chitosan (Chi) functionalized cysteine (cys)-SAM (self-assembled monolayer) based electrochemical, label free, ultrasensitive, non-invasive immunosensing nanomolecular platform has been fabricated for the detection of novel OC biomarker S-100. This has been achieved through bio-functionalization of AgNPs-Chi/cys/mini gold slides by covalent immobilization of anti-S100 antibodies. Cyclic voltammetric (CV) studies establish successful fabrication of anti-S100/AgNPs-Chi/Au nanomolecular platform. Differential pulse voltammetric (DPV) measurements reveal that anti-S100/Chi-AgNPs/Au is able to sense 50 fg mL<sup>-1</sup> – 500 ng mL<sup>-1</sup> of S100 in buffer and in spiked saliva samples within 120 s interaction time having improved favorable shelf life and specificity. The fabricated platform is very selective and able to detect S100 in OC patient/s saliva samples warrants realization of simple non-invasive electrochemical immunosensor for <em>in situ</em> or <em>on-site</em> applications (especially for remote areas) for early diagnosis of OC while offering bio-compatibility at low cost.</p></div>\",\"PeriodicalId\":436,\"journal\":{\"name\":\"Talanta Open\",\"volume\":\"10 \",\"pages\":\"Article 100352\"},\"PeriodicalIF\":4.1000,\"publicationDate\":\"2024-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S2666831924000663/pdfft?md5=3345a9daaf0246157a50c3f630bbc45e&pid=1-s2.0-S2666831924000663-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Talanta Open\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2666831924000663\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Talanta Open","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2666831924000663","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
Fabrication of nanomolecular platform based immunosensor for non-invasive electrochemical detection of oral cancer: An in vitro study
Silver nanoparticles (AgNPs)-Chitosan (Chi) functionalized cysteine (cys)-SAM (self-assembled monolayer) based electrochemical, label free, ultrasensitive, non-invasive immunosensing nanomolecular platform has been fabricated for the detection of novel OC biomarker S-100. This has been achieved through bio-functionalization of AgNPs-Chi/cys/mini gold slides by covalent immobilization of anti-S100 antibodies. Cyclic voltammetric (CV) studies establish successful fabrication of anti-S100/AgNPs-Chi/Au nanomolecular platform. Differential pulse voltammetric (DPV) measurements reveal that anti-S100/Chi-AgNPs/Au is able to sense 50 fg mL-1 – 500 ng mL-1 of S100 in buffer and in spiked saliva samples within 120 s interaction time having improved favorable shelf life and specificity. The fabricated platform is very selective and able to detect S100 in OC patient/s saliva samples warrants realization of simple non-invasive electrochemical immunosensor for in situ or on-site applications (especially for remote areas) for early diagnosis of OC while offering bio-compatibility at low cost.
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