{"title":"H2O2 亚磺酰化 CHE,将局部感染与建立全身获得性抗性联系起来","authors":"Lijun Cao, Sargis Karapetyan, Heejin Yoo, Tianyuan Chen, Musoki Mwimba, Xing Zhang, Xinnian Dong","doi":"10.1126/science.adj7249","DOIUrl":null,"url":null,"abstract":"<div >In plants, a local infection can lead to systemic acquired resistance (SAR) through increased production of salicylic acid (SA). For many years, the identity of the mobile signal and its direct transduction mechanism for systemic SA synthesis in initiating SAR have been debated. We found that in <i>Arabidopsis thaliana</i>, after a local infection, the conserved cysteine residue of the transcription factor CCA1 HIKING EXPEDITION (CHE) undergoes sulfenylation in systemic tissues, which enhances its binding to the promoter of the SA-synthesis gene <i>ISOCHORISMATE SYNTHASE1</i> (<i>ICS1</i>) and increases SA production. Furthermore, hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) produced through NADPH oxidases is the mobile signal that sulfenylates CHE in a concentration-dependent manner. Accumulation of SA and the previously reported signal molecules, such as <i>N</i>-hydroxypipecolic acid (NHP), then form a signal amplification loop to establish SAR.</div>","PeriodicalId":21678,"journal":{"name":"Science","volume":null,"pages":null},"PeriodicalIF":44.7000,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"H2O2 sulfenylates CHE, linking local infection to the establishment of systemic acquired resistance\",\"authors\":\"Lijun Cao, Sargis Karapetyan, Heejin Yoo, Tianyuan Chen, Musoki Mwimba, Xing Zhang, Xinnian Dong\",\"doi\":\"10.1126/science.adj7249\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div >In plants, a local infection can lead to systemic acquired resistance (SAR) through increased production of salicylic acid (SA). For many years, the identity of the mobile signal and its direct transduction mechanism for systemic SA synthesis in initiating SAR have been debated. We found that in <i>Arabidopsis thaliana</i>, after a local infection, the conserved cysteine residue of the transcription factor CCA1 HIKING EXPEDITION (CHE) undergoes sulfenylation in systemic tissues, which enhances its binding to the promoter of the SA-synthesis gene <i>ISOCHORISMATE SYNTHASE1</i> (<i>ICS1</i>) and increases SA production. Furthermore, hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) produced through NADPH oxidases is the mobile signal that sulfenylates CHE in a concentration-dependent manner. Accumulation of SA and the previously reported signal molecules, such as <i>N</i>-hydroxypipecolic acid (NHP), then form a signal amplification loop to establish SAR.</div>\",\"PeriodicalId\":21678,\"journal\":{\"name\":\"Science\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":44.7000,\"publicationDate\":\"2024-09-12\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Science\",\"FirstCategoryId\":\"103\",\"ListUrlMain\":\"https://www.science.org/doi/10.1126/science.adj7249\",\"RegionNum\":1,\"RegionCategory\":\"综合性期刊\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"MULTIDISCIPLINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Science","FirstCategoryId":"103","ListUrlMain":"https://www.science.org/doi/10.1126/science.adj7249","RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MULTIDISCIPLINARY SCIENCES","Score":null,"Total":0}
引用次数: 0
摘要
在植物中,局部感染可通过增加水杨酸(SA)的产生而导致全身获得性抗性(SAR)。多年来,人们一直在争论启动 SAR 的移动信号及其系统性 SA 合成的直接传导机制。我们发现,在拟南芥中,局部感染后,转录因子 CCA1 HIKING EXPEDITION(CHE)的保守半胱氨酸残基在系统组织中发生亚磺酰化,从而增强其与 SA 合成基因 ISOCHORISMATE SYNTHASE1(ICS1)启动子的结合,增加 SA 的产生。此外,通过 NADPH 氧化酶产生的过氧化氢(H2O2)是一种移动信号,能以浓度依赖的方式使 CHE 亚磺酰化。SA 和之前报道的信号分子(如 N-hydroxypipecolic acid (NHP))的积累会形成一个信号放大环,从而建立 SAR。
H2O2 sulfenylates CHE, linking local infection to the establishment of systemic acquired resistance
In plants, a local infection can lead to systemic acquired resistance (SAR) through increased production of salicylic acid (SA). For many years, the identity of the mobile signal and its direct transduction mechanism for systemic SA synthesis in initiating SAR have been debated. We found that in Arabidopsis thaliana, after a local infection, the conserved cysteine residue of the transcription factor CCA1 HIKING EXPEDITION (CHE) undergoes sulfenylation in systemic tissues, which enhances its binding to the promoter of the SA-synthesis gene ISOCHORISMATE SYNTHASE1 (ICS1) and increases SA production. Furthermore, hydrogen peroxide (H2O2) produced through NADPH oxidases is the mobile signal that sulfenylates CHE in a concentration-dependent manner. Accumulation of SA and the previously reported signal molecules, such as N-hydroxypipecolic acid (NHP), then form a signal amplification loop to establish SAR.
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