基于 UHPLC-Q-TOF-MS 的生姜粉成分分析及治疗糖尿病肾病的网络药理学策略

IF 1.5 4区 医学 Q4 CHEMISTRY, MEDICINAL Natural Product Communications Pub Date : 2024-09-10 DOI:10.1177/1934578x241277543
Yongzhi Yin, Lu Yang, Ke Zhang, Silin Yu, Jinfa Liao, Jinhui Wang
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引用次数: 0

摘要

目的生姜粉(SJP)是一种传统的中药复方,常用于治疗糖尿病肾病(DKD)。然而,其物质基础和作用机制尚不清楚。研究方法采用超高效液相色谱-四极杆飞行时间质谱(UHPLC-Q-TOF-MS)分析其体内外成分,并结合分子对接建立网络药理学策略,研究其治疗糖尿病肾病的作用机制。研究结果结果发现了 162 种成分,其中 28 种可被血液吸收并在体内发挥治疗作用。主要活性成分为 CT-1、CT-2、CT-7、BJC-2 和 BJC-5,体内主要靶点与 AKT1、MAPK1、MAPK3、MAPK8 和 MAPK14 有关,可通过 PI3K-Akt 信号通路、胰岛素抵抗、糖尿病并发症中的 AGE-RAGE 信号通路等发挥治疗作用 结论:本研究采用综合分析策略,揭示了SJP治疗DKD的药效物质基础和作用机制,为其临床应用提供了科学依据。
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Compositional Analysis and Network Pharmacological Strategy for the Treatment of Diabetic Kidney Disease by Shengjiang Powder Based on UHPLC-Q-TOF-MS
Objective: Shengjiang Powder (SJP) is a traditional Chinese medicine compound formula commonly used in the treatment of diabetic kidney disease (DKD). However, its material basis and mechanism of action are still unclear. Methods: In this study, the aqueous extract of SJP was obtained by aqueous decoction method, and the in vivo and in vitro constituents of SJP were analysed by ultra-high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS), and a network pharmacological strategy was established in conjunction with molecular docking in order to investigate the mechanism of action of SJP in the treatment of DKD. Results: The results identified 162 components, of which 28 could be absorbed into the blood and exert therapeutic effects in vivo. The main active ingredients were CT-1, CT-2, CT-7, BJC-2, and BJC-5, and the main targets in vivo were related to AKT1, MAPK1, MAPK3, MAPK8, and MAPK14, which could exert their therapeutic effects through the PI3K-Akt signaling pathway, Insulin resistance, and AGE-RAGE signaling pathway in diabetic complications, etc Conclusion: The present study adopts a comprehensive analytical strategy to reveal the pharmacodynamic material basis and mechanism of action of SJP in the treatment of DKD, and to provide a scientific basis for its clinical application.
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来源期刊
Natural Product Communications
Natural Product Communications 工程技术-食品科技
CiteScore
3.10
自引率
11.10%
发文量
254
审稿时长
2.7 months
期刊介绍: Natural Product Communications is a peer reviewed, open access journal studying all aspects of natural products, including isolation, characterization, spectroscopic properties, biological activities, synthesis, structure-activity, biotransformation, biosynthesis, tissue culture and fermentation. It covers the full breadth of chemistry, biochemistry, biotechnology, pharmacology, and chemical ecology of natural products. Natural Product Communications is a peer reviewed, open access journal studying all aspects of natural products, including isolation, characterization, spectroscopic properties, biological activities, synthesis, structure-activity, biotransformation, biosynthesis, tissue culture and fermentation. It covers the full breadth of chemistry, biochemistry, biotechnology, pharmacology, and chemical ecology of natural products. Natural Product Communications is a peer reviewed, open access journal studying all aspects of natural products, including isolation, characterization, spectroscopic properties, biological activities, synthesis, structure-activity, biotransformation, biosynthesis, tissue culture and fermentation. It covers the full breadth of chemistry, biochemistry, biotechnology, pharmacology, and chemical ecology of natural products.
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