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The Influence of Weather Conditions on the Immortelle Volatile Constituents from Essential oil and Hydrosol with a Focus on Italidiones and Its Molecular Docking Anti-Inflammatory Potential 天气条件对仙人掌精油和水溶液中挥发性成分的影响,重点是它的缩水甘油醚及其分子对接抗炎潜力
IF 1.8 4区 医学 Q4 CHEMISTRY, MEDICINAL Pub Date : 2024-09-19 DOI: 10.1177/1934578x241284735
Milica Aćimović, Jovana Stanković Jeremić, Marina Todosijević, Mirjana Cvetković, Biljana Lončar, Vladimir Vukić, Tamara Erceg, Lato Pezo, Valtcho D. Zheljazkov
ObjectivesImmortelle has garnered global attention for its cosmetic, medicinal, and culinary applications worldwide. Apart from neryl acetate, α-pinene and γ-curcumene as the most important components for the estimation of the essential oil quality, in recent years a group of β-diketones, known as italidiones, appear as significant quality parameters. However, it is unknown how weather conditions (temperature and precipitation) influence their accumulation in essential oil and hydrosol, nor their anti-inflammatory potential.MethodsThis study investigates the chemical composition of immortelle essential oil and hydrosol over three successive years (2019/20, 2020/21, and 2021/22), by GC-FID and GC-MS analysis. In silico molecular docking model with Vascular Adhesion Protein-1 (VAP-1) was used for the simulation of the anti-inflammatory potential of italidiones, as well as other β-diketones present in immortelle essential oil and hydrosol.ResultsDuring the three-year trial, it was established that essential oil compounds such as α-pinene, neryl acetate, and italicene were positively correlated with temperature and negatively correlated with precipitation, as well as β-diketones and total italidiones. Moreover, trans-caryophyllene, γ-curcumene, and ar-curcumene were in negative correlation with temperature, and a slight positive correlation with precipitation. On the other side, in the hydrosol the content of both total β-diketones and total italidiones was strongly negatively correlated with temperatures, and positively correlated with precipitation, which was the opposite of essential oil. Additionally, findings indicated that italidiones could bind the human VAP-1 protein in the vicinity of the topiquinone and block its function.ConclusionWeather conditions affect variations in the accumulation of volatile compounds in essential oil and hydrosol, which in some cases can affect changes in olfactory characteristics, as well as criteria prescribed by standards, and even changes in biological activities. These results indicate that italidiones, as well as other β-diketones present in immortelle, may be responsible for its anti-inflammatory activity.
目的墨旱莲因其在美容、医药和烹饪方面的应用而备受全球关注。除了乙酸橙花酯、α-蒎烯和γ-莪术烯是评估精油质量的最重要成分外,近年来,一组被称为 italidiones 的 β-二酮也成为重要的质量参数。本研究通过 GC-FID 和 GC-MS 分析方法,对连续三年(2019/20、2020/21 和 2021/22)的仙客来精油和水醇的化学成分进行了研究。结果在为期三年的试验中,α-蒎烯、乙酸橙花酯、伊塔里烯等精油化合物与温度呈正相关,与降水呈负相关,β-二酮类化合物和总伊塔里酮类化合物也与温度呈正相关,与降水呈负相关。此外,反式叶绿素、γ-莪术烯和 ar-莪术烯与温度呈负相关,与降水略呈正相关。另一方面,在水醇中,总 β-二酮和总 italidiones 的含量与温度呈强烈的负相关,而与降水量呈正相关,这与精油正好相反。结论 天气条件会影响精油和水溶胶中挥发性化合物的积累变化,在某些情况下会影响嗅觉特征的变化以及标准规定的标准,甚至会影响生物活性的变化。这些结果表明,长春花中的 italidiones 以及其他 β-二酮可能是其抗炎活性的原因。
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引用次数: 0
Exploring the Mechanism of Huoxiang Zhengqi Oral Liquid Against Mosquito Bite Dermatitis Through Network Pharmacology and in Vitro Validation 通过网络药理学和体外验证探索藿香正气口服液防治蚊虫叮咬皮炎的机制
IF 1.8 4区 医学 Q4 CHEMISTRY, MEDICINAL Pub Date : 2024-09-17 DOI: 10.1177/1934578x241264621
Kunqin Ma, Xu Xu, Shengdong Wang, Chang Liu, Liping Liu
Objective: Traditional Chinese patent medicine Huoxiang Zhengqi Oral Liquid (HXZQOL) can be used topical to treat mosquito bite dermatitis (MBD). The study aimed to explore the potential mechanism of HXZQOL in treatment of MBD based on network pharmacology and experimental validation. Methods: TCMSP, SwissTargetPrediction and PubChem database were used to predict the active compounds and targets of HXZQOL and GeneCards database was utilized to predict the potential targets of MBD. Target interaction analysis was performed using Venn database, PPI and core targets were assessed using String database, and Cytoscape. Enrichment analysis on Gene Ontology(GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways were performed using David online tool. “HXZQOL-compound-target-pathway-MBD” networks were constructed using Cytoscape. Molecular docking was carried out with Autodock vina. The inflammation model of HaCaT cells induced by TNF-a/IFN-γ was established .to validate the mechanism of HXZQOL against MBD, with the levels of inflammatory factors and protein related to IL-7 signaling pathway assessed using Elisa and Western Blot. Results: HXZQOL contained 154 active compounds and shared 88 common targets with MBD. Quercetin, apigenin, ursolic acid and luteolin were identified as potential candidate compounds, while JUN, IL-6, TNF and VEGFA were suggested as main potential targets. HXZQOL was found to act on MBD through multiple pathways including IL-17, AGE-RAGE and TNF signaling pathways. The candidate compounds demonstrated effectively binding to JUN and IL-6 targets, with binding energies below −5 kcal/mol. Western blot experiments confirmed that these compounds could down-regulate the expression of JUN protein in IL-17 signaling pathway, and significantly reduce levels of inflammatory factors TNF-α, IL-6 and NO in HaCaT cells. Conclusion: HXZQOL mainly exerts its anti MAD effect by reducing inflammation. The relevant results have laid the foundation for improving the local anti MBD effect of HXZQOL and the secondary development of HXZQOL formulations.
目的中成药藿香正气口服液(HXZQOL)可外用治疗蚊虫叮咬性皮炎(MBD)。本研究旨在基于网络药理学和实验验证,探讨 HXZQOL 治疗 MBD 的潜在机制。研究方法利用 TCMSP、SwissTargetPrediction 和 PubChem 数据库预测 HXZQOL 的活性化合物和靶点,利用 GeneCards 数据库预测 MBD 的潜在靶点。使用 Venn 数据库进行了靶点相互作用分析,使用 String 数据库和 Cytoscape 评估了 PPI 和核心靶点。使用 David 在线工具对基因本体(GO)和京都基因组百科全书(KEGG)通路进行了富集分析。使用 Cytoscape 构建了 "HXZQOL-化合物-目标-途径-MBD "网络。分子对接使用 Autodock vina 进行。建立了 TNF-a/IFN-γ 诱导的 HaCaT 细胞炎症模型,用 Elisa 和 Western Blot 评估炎症因子和 IL-7 信号通路相关蛋白的水平,以验证 HXZQOL 抗 MBD 的机制。结果HXZQOL含有154种活性化合物,与MBD有88个共同靶点。槲皮素、芹菜素、熊果酸和木犀草素被确定为潜在的候选化合物,而 JUN、IL-6、TNF 和 VEGFA 被认为是主要的潜在靶点。研究发现,HXZQOL 可通过多种途径作用于 MBD,包括 IL-17、AGE-RAGE 和 TNF 信号途径。候选化合物与 JUN 和 IL-6 靶点有效结合,结合能低于 -5 kcal/mol。Western blot 实验证实,这些化合物可以下调 IL-17 信号通路中 JUN 蛋白的表达,并显著降低 HaCaT 细胞中炎症因子 TNF-α、IL-6 和 NO 的水平。结论HXZQOL主要通过减轻炎症反应来发挥抗MAD作用。相关结果为提高 HXZQOL 的局部抗 MBD 作用和 HXZQOL 制剂的二次开发奠定了基础。
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引用次数: 0
Astragalus Injection Enhances the Sensitivity of Clinical Cancer Patients to Chemotherapy: A Systematic meta-Analysis 黄芪注射液可提高临床癌症患者对化疗的敏感性:系统荟萃分析
IF 1.8 4区 医学 Q4 CHEMISTRY, MEDICINAL Pub Date : 2024-09-17 DOI: 10.1177/1934578x241276966
Tingjie Ye, Xiaofeng Yan, Wenhao Xiu, Changtai Qin, Yuxi Yang, Jinzu Yang, Limin Zhu, Xiaoling Wang, Wei Xu, Yanlin Lu
BackgroundTraditional Chinese Medicine Astragalus was commonly used to assist chemo-drug treatment in clinical cancer patients. However, whether Astragalus injection (AGI) reverses drug resistance remains unclear. Therefore, a meta-analysis is necessary to assess the effect of AGI on resistance reversement in cancer patients.MethodThe studies presenting tumor response rates in cancer patients receiving chemo-drugs treatment in combination with AGI were systematically searched from six common scientific databases until February 2024. The relative risks (RRs) indicating the tumor response rate, 1-year survival rate, and quality of life improvement in clinical patients among two groups were calculated in metan package. The pooled RRs with 95% confidence intervals (CIs) were used to explore the effect of AGI on enhancing drug sensitivity in terms of tumor response, 1-year survival rate and quality of life improvement in cancer patients.ResultFifty-one studies were included for meta-analysis following a thorough screening process that adhered to the inclusion and exclusion criteria. A total of 4613 patients were enrolled in all included trials. Results obtained indicated that AGI significantly enhanced the sensitivity of cancers to chemo-drugs with pooled RRs (95% CI) of 1.25 (1.18, 1.32), prolonged the 1-year survival rate of patients with pooled RRs (95% CI) of 1.34 (1.14, 1.57), improved the quality of life with pooled RRs (95% CI) of 1.61 (1.34, 1.93). Similarly, Astragalus's main component, astragaloside polysaccharides (APS), also enhances drug sensitivity in clinical cancer patients.ConclusionOur findings demonstrated that AGI had the ability to resensitize cancers to chemo-drugs and improve the quality of life of cancer patients. Therefore, AGI could potentially be used as a drug resistance reversal for resistant cancer treatment.
背景传统中药黄芪常用于辅助临床癌症患者的化疗药物治疗。然而,黄芪注射液(AGI)是否能逆转耐药性仍不清楚。方法从6个常见的科学数据库中系统检索了2024年2月之前癌症患者接受化疗药物联合AGI治疗后肿瘤反应率的研究。用 metan 软件包计算两组临床患者肿瘤反应率、1 年生存率和生活质量改善的相对风险(RRs)。结果经过严格筛选,共有 51 项研究被纳入荟萃分析。所有纳入的试验共招募了 4613 名患者。研究结果表明,AGI能显著提高癌症对化疗药物的敏感性,总RRs(95% CI)为1.25(1.18,1.32);延长患者的1年生存率,总RRs(95% CI)为1.34(1.14,1.57);改善生活质量,总RRs(95% CI)为1.61(1.34,1.93)。同样,黄芪的主要成分黄芪皂苷多糖(APS)也能提高临床癌症患者对药物的敏感性。因此,AGI 有可能被用作抗药性癌症治疗的耐药性逆转剂。
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引用次数: 0
Medicinal Plants as Effective Antiviral Agents and Their Potential Benefits 作为有效抗病毒剂的药用植物及其潜在益处
IF 1.8 4区 医学 Q4 CHEMISTRY, MEDICINAL Pub Date : 2024-09-14 DOI: 10.1177/1934578x241282923
Gabriel Atampugbire, Eureka Emefa Ahadjie Adomako, Osbourne Quaye
This paper explores a diverse field of medicinal plants as potential antiviral agents, and delves into utilization of plant medicine for combating viral infections, emphasizing recent surge in research on natural products from plants as antiviral agents. Specific plant-derived compounds, like phyllanthin and iscador, have been proven to exhibit antiviral properties with great potential for pharmaceutical development. Mechanisms of antiviral action by phytochemicals that are present in medicinal plants, including direct viral inhibition, degradation of viral capsid, and immunomodulation were identified, and a combination therapy of medicinal plants with conventional antiviral drugs were explored. Efficacy of medicinal plants as antiviral agents was critically assessed and revealed that the complexity and variability of herbal formulations, and safety concerns regarding toxicity levels, pose challenges in drug development. However, research on medicinal plants is often hindered by limited understanding of phytochemical mechanisms, the complexity and variability of herbal formulations, and safety concerns regarding toxicity and interactions with other medications. The way forward in harnessing full potential of medicinal plants as antiviral agents underscores the need for further research into developing models that seek to enhance the selectivity of plant extracts in order to minimize toxicity levels.
本文探讨了作为潜在抗病毒剂的药用植物的多个领域,并深入研究了利用植物药防治病毒感染的问题,同时强调了近期有关植物天然产品作为抗病毒剂的研究热潮。特定的植物提取物,如植物黄质和异黄质,已被证实具有抗病毒特性,在药物开发方面具有巨大潜力。研究发现了药用植物中的植物化学物质的抗病毒作用机制,包括直接抑制病毒、降解病毒外壳和免疫调节,并探索了药用植物与传统抗病毒药物的联合疗法。对药用植物作为抗病毒药物的疗效进行了严格评估,结果表明,草药配方的复杂性和多变性,以及对毒性水平的安全性担忧,给药物开发带来了挑战。然而,由于对植物化学机制的了解有限、草药配方的复杂性和多变性,以及对毒性和与其他药物相互作用的安全性的担忧,药用植物的研究往往受到阻碍。要充分挖掘药用植物作为抗病毒药物的潜力,就需要进一步研究开发相关模型,以提高植物提取物的选择性,从而最大限度地降低毒性水平。
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引用次数: 0
Exploring the Effects of Different Processing Techniques on the Antitussive Expectorant of Platycodon grandiflorus by Metabonomics and Pharmacologic Methods 通过代谢组学和药理学方法探索不同加工技术对桔梗镇咳祛痰剂的影响
IF 1.8 4区 医学 Q4 CHEMISTRY, MEDICINAL Pub Date : 2024-09-14 DOI: 10.1177/1934578x241284430
Shuting Zhao, Jingying Xv, Miaomiao Tang, Wei Hou
Background and PurposePlatycodon grandiflorum is a traditional Chinese herbal medicine, which can disperse lung, promote pharynx, dispel phlegm and expel pus. Its processing history was first seen in the “Zhou Hou Bei Ji Fang” of the Jin Dynasty in China. Through processing, effective substances in plants can be enriched and negative substances can be reduced. Therefore, this study explored the effects of different processing methods on the antitussive and expectorant effects of Platycodon grandiflorum through metabolomics and pharmacology, aiming to provide a theoretical basis for clinical application.Experimental ApproachThe changes of metabolites before and after processing of Platycodon grandiflorum were analyzed by Nexera UHPLC LC-30A-HILIC-Orbitrap-Q Exactive HF-X platform. The antitussive and expectorant effects of processed Platycodon grandiflorum were verified by mouse concentrated ammonia induced cough method and tracheal phenol red drainage method.Key ResultsAfter processing, some metabolites of Platycodon grandiflorum changed, among which 24 kinds of terpenoid metabolites changed significantly. Through the experiment of ammonia-induced cough in mice and phenol red expectorant experiment, it was found that fresh JZI 40%, fresh CZ 80%, dry JZZ 40% and dry JZI 80% had obvious antitussive effect, and dry JZZ 40% had obvious expectorant effect.Conclusions and ImplicationsSome processing methods can indeed further improve the antitussive and expectorant effects of Platycodon grandiflorum, which may be due to the effect of increasing the content of triterpenoids after processing, but the mechanism of processing to improve the effect of relieving cough and expectorant needs further study.
背景与目的桔梗是一种传统中药材,具有宣肺利咽、祛痰排脓的功效。其加工历史最早见于中国晋代的《周后备急方》。通过加工,可以丰富植物中的有效物质,减少负面物质。实验方法采用Nexera UHPLC LC-30A-HILIC-Orbitrap-Q Exactive HF-X平台分析桔梗加工前后代谢物的变化。结果加工后桔梗的部分代谢物发生了变化,其中24种萜类代谢物变化明显。通过氨气致小鼠咳嗽实验和酚红祛痰实验发现,鲜桔梗40%、鲜桔梗80%、干桔梗40%和干桔梗80%均有明显的镇咳作用,干桔梗40%有明显的祛痰作用。结论与启示有些加工方法确实能进一步提高桔梗的镇咳和祛痰作用,这可能是由于加工后增加了三萜类化合物的含量,但加工提高镇咳和祛痰作用的机理还需要进一步研究。
{"title":"Exploring the Effects of Different Processing Techniques on the Antitussive Expectorant of Platycodon grandiflorus by Metabonomics and Pharmacologic Methods","authors":"Shuting Zhao, Jingying Xv, Miaomiao Tang, Wei Hou","doi":"10.1177/1934578x241284430","DOIUrl":"https://doi.org/10.1177/1934578x241284430","url":null,"abstract":"Background and PurposePlatycodon grandiflorum is a traditional Chinese herbal medicine, which can disperse lung, promote pharynx, dispel phlegm and expel pus. Its processing history was first seen in the “Zhou Hou Bei Ji Fang” of the Jin Dynasty in China. Through processing, effective substances in plants can be enriched and negative substances can be reduced. Therefore, this study explored the effects of different processing methods on the antitussive and expectorant effects of Platycodon grandiflorum through metabolomics and pharmacology, aiming to provide a theoretical basis for clinical application.Experimental ApproachThe changes of metabolites before and after processing of Platycodon grandiflorum were analyzed by Nexera UHPLC LC-30A-HILIC-Orbitrap-Q Exactive HF-X platform. The antitussive and expectorant effects of processed Platycodon grandiflorum were verified by mouse concentrated ammonia induced cough method and tracheal phenol red drainage method.Key ResultsAfter processing, some metabolites of Platycodon grandiflorum changed, among which 24 kinds of terpenoid metabolites changed significantly. Through the experiment of ammonia-induced cough in mice and phenol red expectorant experiment, it was found that fresh JZI 40%, fresh CZ 80%, dry JZZ 40% and dry JZI 80% had obvious antitussive effect, and dry JZZ 40% had obvious expectorant effect.Conclusions and ImplicationsSome processing methods can indeed further improve the antitussive and expectorant effects of Platycodon grandiflorum, which may be due to the effect of increasing the content of triterpenoids after processing, but the mechanism of processing to improve the effect of relieving cough and expectorant needs further study.","PeriodicalId":19019,"journal":{"name":"Natural Product Communications","volume":"53 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142249261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
NingXueShengBan Decoction Regulates Immune Homeostasis by PI3 K/Akt/mTOR and TLR4/NF-κB Signaling Pathways in Immune Thrombocytopenia Patients 宁雪生煎通过 PI3 K/Akt/mTOR 和 TLR4/NF-κB 信号通路调节免疫性血小板减少症患者的免疫平衡
IF 1.8 4区 医学 Q4 CHEMISTRY, MEDICINAL Pub Date : 2024-09-14 DOI: 10.1177/1934578x241276960
Haijin Wang, Mengxiao Wang, Wuxia Yang, Runfeng Ni, Lin Ma, Dan Wang, Chao Yan, Yuhong Wu, Baoshan Liu, Aidi Wang
ObjectiveImmune thrombocytopenia (ITP) is an autoimmune hemorrhagic disease. The immune imbalance of T cells is an essential pathological mechanism of ITP, more specific, the balance of Effector T cells (Teff)/Regulatory T cell (Treg) maintains T cell immune homeostasis. Dendritic cells (DC), the largest antigen-presenting cell, interfere with T cell differentiation pathways and exert immune regulatory functions. Our team created and applied the “ NingXueShengBan” (NXSB) decoction. According to our prior studies, we found that NXSB decoction could restore Th17/Treg balance in ITP mice by regulating protein expression in Notch pathway. Therefore, we further research the mechanism of NXSB decoction that regulates ITP immune homeostasis through affecting the interaction between DC and T cells.MethodsIn this study, we collected peripheral blood from health donors and ITP patients and extracted DC and CD4 + T cells then grouped in control group, model group and NXSB group. Moreover, we established a co-culture model simulating the immune environment in ITP patient to further investigate whether NXSB had therapeutic effects by the interaction between DC and CD4 + T cells.ResultsIn the current study, we found that NXSB decoction potently regulated interaction between DC and T cells by inhibiting DC development and function, specifically, the differentiation of CD4+ T cells was inhibited after NXSB decoction intervened. Our data showed that NXSB decoction inhibited Toll-like receptor 4 (TLR-4), reduced the expression of Nuclear factor kappa-B (NF-κB) and led to the reduction of downstream inflammatory factors, thereby inhibiting the inflammatory response. More importantly, NXSB decoction reduced the DC surface expression of activation and maturation markers. Moreover, we detected that the decreased expression of the phosphorylated Phosphatidylinositol 3-kinase (PI3 K), Protein kinase B (Akt), Mammalian target of rapamycin (mTOR), and their downstream effectors indicated, we found that NXSB decoction could inhibit PI3 K/Akt/mTOR signaling pathways significantly.ConclusionsCollectively, our data suggested that NXSB decoction ameliorates ITP-induced immune imbalance via its anti-inflammation and immunosuppressive by regulating TLR-4/ NF-κB and PI3 K/Akt/mTOR signaling pathways.
目的免疫性血小板减少症(ITP)是一种自身免疫性出血性疾病。T细胞的免疫失衡是ITP的重要病理机制,具体而言,效应T细胞(Teff)/调节T细胞(Treg)的平衡维持着T细胞的免疫平衡。树突状细胞(DC)是最大的抗原递呈细胞,能干扰T细胞分化途径并发挥免疫调节功能。我们的团队创造并应用了 "宁血生煎"。根据之前的研究,我们发现宁血生煎可通过调节 Notch 通路蛋白的表达,恢复 ITP 小鼠 Th17/Treg 的平衡。因此,我们进一步研究了NXSB煎剂通过影响DC和T细胞之间的相互作用来调节ITP免疫平衡的机制。结果 在本研究中,我们发现 NXSB 水煎剂通过抑制 DC 的发育和功能,有效调节 DC 和 T 细胞之间的相互作用,特别是在 NXSB 水煎剂干预后,CD4+ T 细胞的分化受到抑制。我们的数据显示,NXSB 水煎剂抑制了 Toll 样受体 4(TLR-4),降低了核因子卡巴-B(NF-κB)的表达,导致下游炎症因子减少,从而抑制了炎症反应。更重要的是,NXSB 水煎剂降低了直流电表面活化和成熟标志物的表达。此外,我们还检测到磷酸化磷脂酰肌醇3-激酶(PI3 K)、蛋白激酶B(Akt)、哺乳动物雷帕霉素靶标(mTOR)及其下游效应物的表达减少,表明NXSB水煎剂能显著抑制PI3 K/Akt/mTOR信号通路。结论综上所述,我们的数据表明,NXSB煎剂通过调节TLR-4/ NF-κB和PI3 K/Akt/mTOR信号通路,具有抗炎和免疫抑制作用,可改善ITP诱导的免疫失衡。
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引用次数: 0
To Explore the Rule and Mechanism of Traditional Chinese Medicine in the Treatment of Postmenopausal Osteoporosis Based on Data Mining, Network Pharmacology and Molecular Docking Technology 基于数据挖掘、网络药理学和分子对接技术,探索中药治疗绝经后骨质疏松症的规律和机制
IF 1.8 4区 医学 Q4 CHEMISTRY, MEDICINAL Pub Date : 2024-09-14 DOI: 10.1177/1934578x241281303
Hao Zeng, Keqi Huang, Yuan Chai, Hua Liu, Yongjin Li, Zhengpeng Li, Xiaoyun Zhang
Postmenopausal osteoporosis (PMOP) is an important part of primary osteoporosis, and the current clinical treatment program for PMOP is easily limited by side effects and adverse reactions, so it is particularly important to seek more efficient, safe, and economical drug treatment for PMOP. In recent years, with the change of disease treatment mode and the gradual deepening of Traditional Chinese medicine (TCM) research on PMOP, a review of the literature reveals that there is a huge number of studies on the use of TCM in the treatment of PMOP, but there are no relevant systematic studies on the rules of its formulas and the specific mechanisms by which the core drugs exert their therapeutic effects. Therefore, in this study, we collected a total of 141 formulas used by TCM clinicians to treat PMOP, statistically analyzed their high-frequency medications, Four Qi, Five Flavors, meridians, and efficacies, and analyzed the core medications based on the association rules, which were Rehmanniae Radix Praeparata, Epimedium, Eucommia ulmoides, Rhizoma Drynariae, Angelica sinensis, Achyranthes, Astragalus propinquus, Psoralen, Cornus officinalis and licorice. A total of 87 drug pairs were obtained from the correlation analysis, and a total of 8 groups of potential core combination drugs and 4 potential new prescriptions were derived based on the cluster analysis. Subsequently, a network pharmacological analysis of the core drugs was conducted to obtain 173 active ingredients in the core drugs, including kaempferol, β-sitosterol, quercetin, etc. 298 targets of action, including MAPK3, STAT1, HSP90AA1, etc. 170 signaling pathways including AGE-RAGE, PI3 K/Akt, TNF, HIF-1 and others. The molecular docking results showed that 11 key active ingredients in the core drugs had stable binding to the target targets. This study showed that TCM treatment of PMOP is mainly based on “reinforcing liver and kidney, warmly invigorating spleen and stomach, activating blood and resolving stasis.” Core drugs therapy for PMOP is a comprehensive intervention through multi-component, multi-target, and multi-pathway approaches. The minimum binding energies between the 11 key active ingredients and the 11 key targets were calculated in a comprehensive analysis, which shows that the core drugs have good binding activities to their therapeutic targets, proving that the predictions of this study are reliable. In the future, it will provide certain medication basis and data support for TCM treatment of PMOP.
绝经后骨质疏松症(PMOP)是原发性骨质疏松症的重要组成部分,目前临床上对PMOP的治疗方案容易受到副作用和不良反应的限制,因此寻求更高效、安全、经济的药物治疗PMOP显得尤为重要。近年来,随着疾病治疗模式的转变和中医药对原发性骨髓瘤研究的逐步深入,查阅文献发现,关于中医药治疗原发性骨髓瘤的研究数量庞大,但对其组方规律及核心药物发挥疗效的具体机制尚无相关的系统研究。因此,在本研究中,我们共收集了141个中医临床医生治疗原发性骨髓瘤的方剂,统计分析了其高频药物、四气、五味、经络和功效、并根据关联规则分析了地黄、淫羊藿、杜仲、黄连、当归、牛膝、黄芪、车前子、山茱萸、甘草等核心药物。相关性分析共得出 87 对药物,根据聚类分析共得出 8 组潜在的核心复方药物和 4 个潜在的新处方。随后,对核心药物进行了网络药理学分析,得到了核心药物中的 173 种有效成分,包括山奈酚、β-谷甾醇、槲皮素等。298 个作用靶点,包括 MAPK3、STAT1、HSP90AA1 等。170 个信号通路,包括 AGE-RAGE、PI3 K/Akt、TNF、HIF-1 等。分子对接结果显示,核心药物中的11种关键活性成分与靶标有稳定的结合。该研究表明,中医治疗原发性血小板增多症以 "补肝肾、温脾胃、活血化瘀 "为主。核心药物治疗原发性骨髓瘤是通过多成分、多靶点、多途径的综合干预。通过综合分析,计算出11种关键有效成分与11个关键靶点的最小结合能,表明核心药物与其治疗靶点具有良好的结合活性,证明本研究的预测结果是可靠的。今后,将为中医药治疗原发性骨髓瘤提供一定的用药依据和数据支持。
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引用次数: 0
The Components of Buyang Huanwu Decoction with UPLC-MS 用 UPLC-MS 分析步阳黄乌煎膏的成分
IF 1.8 4区 医学 Q4 CHEMISTRY, MEDICINAL Pub Date : 2024-09-14 DOI: 10.1177/1934578x241275820
Shijian Fang, Yuxue Ma, Shuaihu Yang, Ruihong Yang, Xingtong Chen, Jinbiao Yang, Yunyue Zhou, Hongbin Xiao, Yukun Zhang, Wenying Niu
Objective: Buyang-Huanwu Decoction is often used as an adjuvant therapy for ischemic stroke in clinical practice. At the same time, various laboratories have found that it has the potential to treat ischemic heart failure, lower extremity varicose veins, pulmonary hypertension, pulmonary fibrosis, and hyperlipidemia. Previous studies in our laboratory have found that different concentrations of ethanol were used to separate the polar components of Buyang-Huanwu decoction, and the therapeutic effects of each group on pulmonary fibrosis and hyperlipidemia were different. Therefore, we analyzed and explored Buyang Huanwu decoction and its products. Methods: The isolated components were obtained by treatment with absolute ethanol, 30% (v/v), 50% (v/v), 75% (v/v), and 90% (v/v) ethanol, and the components were analyzed by UPLC-MS. Results: The separation method used in our laboratory could well separate the components of Buyang Huanwu Decoction, and simply enrich carbohydrates, organic acids, phenylpropanoid compounds, lipids, nucleotides, and lignans. Conclusions: The products treated with different concentrations of ethanol have different therapeutic potentials. The high content components of 30% ethanol eluate and 50% ethanol eluate have good therapeutic effects on osteoarthritis and bone loss, while the high content components of the precipitate components and 75% eluate are more likely to be related to glucose and lipid metabolism disorders and cardiovascular diseases.
目的:步阳黄芪汤在临床上常被用作缺血性中风的辅助治疗。同时,多个实验室发现其具有治疗缺血性心力衰竭、下肢静脉曲张、肺动脉高压、肺纤维化和高脂血症的潜力。我们实验室之前的研究发现,用不同浓度的乙醇分离步阳黄乌煎膏的极性成分,各组对肺纤维化和高脂血症的治疗效果不同。因此,我们对步阳黄乌煎及其制品进行了分析和探讨。方法用绝对乙醇、30%(v/v)、50%(v/v)、75%(v/v)和90%(v/v)乙醇分别处理,分离得到布央黄芪煎剂及其制品,并用UPLC-MS对其成分进行分析。结果本实验室采用的分离方法能很好地分离出布依黄乌煎膏的成分,并简单地富集了碳水化合物、有机酸、苯丙类化合物、脂类、核苷酸和木脂素。结论经不同浓度乙醇处理的产品具有不同的治疗潜力。30%乙醇洗脱液和50%乙醇洗脱液中的高含量成分对骨关节炎和骨质疏松有很好的治疗效果,而沉淀成分和75%洗脱液中的高含量成分更可能与糖脂代谢紊乱和心血管疾病有关。
{"title":"The Components of Buyang Huanwu Decoction with UPLC-MS","authors":"Shijian Fang, Yuxue Ma, Shuaihu Yang, Ruihong Yang, Xingtong Chen, Jinbiao Yang, Yunyue Zhou, Hongbin Xiao, Yukun Zhang, Wenying Niu","doi":"10.1177/1934578x241275820","DOIUrl":"https://doi.org/10.1177/1934578x241275820","url":null,"abstract":"Objective: Buyang-Huanwu Decoction is often used as an adjuvant therapy for ischemic stroke in clinical practice. At the same time, various laboratories have found that it has the potential to treat ischemic heart failure, lower extremity varicose veins, pulmonary hypertension, pulmonary fibrosis, and hyperlipidemia. Previous studies in our laboratory have found that different concentrations of ethanol were used to separate the polar components of Buyang-Huanwu decoction, and the therapeutic effects of each group on pulmonary fibrosis and hyperlipidemia were different. Therefore, we analyzed and explored Buyang Huanwu decoction and its products. Methods: The isolated components were obtained by treatment with absolute ethanol, 30% (v/v), 50% (v/v), 75% (v/v), and 90% (v/v) ethanol, and the components were analyzed by UPLC-MS. Results: The separation method used in our laboratory could well separate the components of Buyang Huanwu Decoction, and simply enrich carbohydrates, organic acids, phenylpropanoid compounds, lipids, nucleotides, and lignans. Conclusions: The products treated with different concentrations of ethanol have different therapeutic potentials. The high content components of 30% ethanol eluate and 50% ethanol eluate have good therapeutic effects on osteoarthritis and bone loss, while the high content components of the precipitate components and 75% eluate are more likely to be related to glucose and lipid metabolism disorders and cardiovascular diseases.","PeriodicalId":19019,"journal":{"name":"Natural Product Communications","volume":"12 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142249211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
LC-MS/MS Profiling, Antioxidant Potential and Cytotoxicity Evaluation of Citrus reticulata Albedo 柑橘网纹叶的 LC-MS/MS 分析、抗氧化潜力和细胞毒性评估
IF 1.8 4区 医学 Q4 CHEMISTRY, MEDICINAL Pub Date : 2024-09-14 DOI: 10.1177/1934578x241272471
Javaid Aftab, Mazhar Abbas, Sumaira Sharif, Aqsa Mumtaz, Kinza Zafar, Naveed Ahmad, Osama A. Mohammed, Arif Nazir, Shahid Iqbal, Munawar Iqbal
Background: The massive production of agro-industrial waste has become a global concern. Approximately 3.6 million tons of citrus waste are disposed of annually after processing, despite most bioactive molecules being found in fruit peels. Objectives/methodology: This work explored the biochemical and carbohydrate polymer profile of methanolic extract of the soft, spongy Citrus reticulata ( C. reticulata) albedo through proximate analysis, antioxidant and scavenging potential analysis, carbohydrate analysis, antioxidant enzyme and genotoxicity analysis, which were further quantified by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay and Fourier transform infrared spectroscopy (FTIR) analysis. Results: The results revealed that the citrus albedo comprised an ample amount of ash (3.30 g/100−g of fresh weight (FW), moisture (52.67 g/100−g FW), fiber (30.80 g/100−g FW), lipid (5.58 g/100−g FW), protein (9.62 Kcal/ 100−g FW) and carbohydrate contents, ie, pectin (18.5 mg/g of dry weight (DW), glucosamine (5.05 mg/g DW), maltose (6.5 mg/g DW), D-xylose (35.5 mg/g DW), cellulose (12.2 mg/g DW), total soluble sugars (13.5 mg/g DW), starch (29% w/w). The antioxidant enzyme exhibited excellent activities (23.06 IU/mL for superoxide dismutase, 37.10 IU/mL for catalase and 1.27 IU/mL for peroxidase). The bioactive molecules of the citrus albedo showed maximum total phenolic content (TPC) (2.30 mg gallic acid equivalent (GAE)/ g DW), total flavonoid contents (TFC) (1.71 mg quercetin equivalent (QE)/ g DW) and scavenging potential (50.67% inhibition) when used at a higher concentration of 100 mg/mL. FTIR and liquid chromatography-mass spectrometry (LC-MS) findings concluded that the citrus albedo was rich in polysaccharides including pectin, glucosamine and antioxidants like caffeic acid, quercitin, naringenin, hesperidin and resveratrol. Moreover, the MTT assay showed that the hepatoblastoma cell line (HepG2) underwent apoptosis after 72-h exposure to C. reticulata albedo extract at varied concentrations. Conclusion: These findings suggest that citrus albedo can be a potential chemotherapeutic agent against hepatocellular carcinoma.
背景:农用工业废物的大量产生已成为全球关注的问题。尽管大多数生物活性分子都存在于果皮中,但每年约有 360 万吨柑橘加工后的废弃物被丢弃。目标/方法:这项研究探索了软海绵柑橘(C. reticulata)反皮层甲醇提取物的生物化学和碳水化合物聚合物特征。通过近似物分析、抗氧化和清除潜能分析、碳水化合物分析、抗氧化酶和遗传毒性分析,并通过 MTT(3-[4,5-二甲基噻唑-2-基]-2,5-二苯基溴化四氮唑)测定法和傅立叶变换红外光谱(FTIR)分析进一步对其进行量化。结果显示结果表明,柑橘反照素含有大量灰分(3.30 克/100 克鲜重(FW))、水分(52.67 克/100 克 FW)、纤维(30.80 克/100 克 FW)、脂质(5.58 克/100 克 FW)、蛋白质(9.碳水化合物含量,即果胶(18.5 毫克/克干重)、葡萄糖胺(5.05 毫克/克干重)、麦芽糖(6.5 毫克/克干重)、D-木糖(35.5 毫克/克干重)、纤维素(12.2 毫克/克干重)、总可溶性糖(13.5 毫克/克干重)、淀粉(29% w/w)。抗氧化酶表现出极高的活性(超氧化物歧化酶为 23.06 IU/mL,过氧化氢酶为 37.10 IU/mL,过氧化物酶为 1.27 IU/mL)。当使用浓度为 100 毫克/毫升时,柑橘白皮中的生物活性分子显示出最大的总酚含量(TPC)(2.30 毫克没食子酸当量(GAE)/克干重)、总黄酮含量(TFC)(1.71 毫克槲皮素当量(QE)/克干重)和清除潜力(50.67% 的抑制率)。傅立叶变换红外光谱和液相色谱-质谱法(LC-MS)的研究结果表明,柑橘白皮含有丰富的多糖,包括果胶、葡萄糖胺和抗氧化剂,如咖啡酸、槲皮苷、柚皮苷、橙皮苷和白藜芦醇。此外,MTT 试验表明,肝母细胞瘤细胞株(HepG2)在暴露于不同浓度的网纹叶蝉提取物 72 小时后出现凋亡。结论这些研究结果表明,柑橘反照素是一种潜在的肝细胞癌化疗药物。
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引用次数: 0
Essential Oils from Phlomis longifolia Boiss. & C.I. Blanche. Aerial Parts (Flowers, Calyxes, Leaves): GC-MS Analyzes and Biological Properties Phlomis longifolia Boiss.气生部分(花、花萼、叶):GC-MS 分析和生物特性
IF 1.8 4区 医学 Q4 CHEMISTRY, MEDICINAL Pub Date : 2024-09-14 DOI: 10.1177/1934578x241282866
Muhannad Hasan, Imad Hwija, Yaseer Mossa
Objective/Background: Phlomis longifolia Boiss. & C.I. Blanche. is one of the significant medicinal plants extensively utilized in folk medicine in Syria. So, this study aimed to identify the chemical components with potential pharmacological properties of essential oils extracted from the aerial parts of the Syrian P. longifolia plant for the first time. Methods: The aerial parts of the plant were collected from a mountainous area in Latakia Province, Syria. Subsequently, the essential oils were obtained using hydrodistillation with a yield of (0.14% for flowers, 0.075% for calyxes, and 0.19% for leaves) using a Clevenger-type apparatus and analyzed by gas chromatography coupled with mass spectrometry (GC-MS). Results: A total of 63, 61, and 48 compounds, which represent (98.0%, 97.1%, and 97.9%) of the total oils, were identified for flowers, calyxes, and leaves, respectively. The major compounds identified in the flower's essential oil were: widdrol (29.8%), β-caryophyllene (9.7%), and (E)-nerolidyl acetate (5.8%). While in the calyx's essential oil were: (E)-nerolidyl acetate (8.6%), α-humulene (8.1%), and β-caryophyllene (7.7%). As for the leave's essential oil were: (E)-nerolidyl acetate (11.4%), β-caryophyllene (9.5%), α-amorphene (8.8%), caryophyllene oxide (6.7%), α-humulene (5.3%), and 3,5-dihydroxy-6-methyl-2,3-dihydro-4H-pyran-4-one (5.2%). Additionally, the majority of the identified compounds have various biological activities, according to the published literature. Conclusion: In this work, the chemical composition of essential oils extracted from the aerial parts of P. longifolia was determined for the first time by GC-MS. Terpenes were the dominant chemical content of essential oils (83.3% for flowers, 72.7% for calyxes, and 80.9 for leaves), and sesquiterpenes had the highest concentration among them (77.3% for flowers, 65.7% for calyxes, and 77.1% for leaves). These compounds are known for their diverse biological activities and promote the use of such plants in phytopharmaceuticals.
目的/背景:Phlomis longifolia Boiss.因此,本研究旨在首次鉴定从叙利亚长叶白头翁植物气生部分提取的精油中具有潜在药理特性的化学成分。研究方法从叙利亚拉塔基亚省的山区采集了该植物的气生部分。随后,使用 Clevenger 型仪器通过水蒸馏法提取精油(花的收率为 0.14%,萼的收率为 0.075%,叶的收率为 0.19%),并通过气相色谱-质谱联用仪(GC-MS)进行分析。结果:在花、花萼和叶中分别鉴定出 63、61 和 48 种化合物,占油总量的 98.0%、97.1% 和 97.9%。在花的精油中鉴定出的主要化合物有:威得醇(29.8%)、β-茶叶烯(9.7%)和(E)-nerolidyl acetate(5.8%)。而花萼的精油中则有(E)-乙酸nerolidyl酯(8.6%)、α-胡麻烯(8.1%)和β-石竹烯(7.7%)。香叶精油包括(E)-nerolidyl acetate (11.4%)、β-caryophyllene (9.5%)、α-amorphene (8.8%)、caryophyllene oxide (6.7%)、α-humulene (5.3%) 和 3,5-二羟基-6-甲基-2,3-二氢-4H-吡喃-4-酮 (5.2%)。此外,根据已发表的文献,大部分已鉴定化合物都具有不同的生物活性。结论在这项研究中,首次采用气相色谱-质谱法测定了从龙脑香叶(P. longifolia)气生部分提取的精油的化学成分。萜烯类化合物是精油的主要化学成分(花为 83.3%,萼为 72.7%,叶为 80.9%),其中倍半萜类化合物的含量最高(花为 77.3%,萼为 65.7%,叶为 77.1%)。这些化合物以其多样的生物活性而闻名,促进了此类植物在植物药物中的应用。
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Natural Product Communications
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