含有刺五加甙 17 和人参皂苷 Re 的淋巴皂苷乙醇提取物通过靶向 AKT/NF-κB 通路发挥抗炎作用

IF 6.8 2区 医学 Q1 CHEMISTRY, MEDICINAL Journal of Ginseng Research Pub Date : 2024-08-25 DOI:10.1016/j.jgr.2024.08.003
Wooram Choi, Hyun Soo Kim, Donghyun Kim, Yong Deog Hong, Hyoung-June Kim, Ji Hye Kim, Jong-Hoon Kim, Jae Youl Cho
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引用次数: 0

摘要

根据烘干、蒸煮和加热等加工方法,人参被加工成多种类型,如白参、红参和黑参。这些加工条件会改变有用成分的比例。最近,人们建立了新的加工方法,开发出在厌氧条件下制备的陈年新鲜白参 "lymphanax"。研究发现,这种陈化过程增加了人参皂苷 17(Gyp17)和人参皂苷 Re 的含量,而人参皂苷 Re 具有抗炎作用。因此,我们下一步的目标是利用淋巴杉乙醇提取物(Lymphanax-EE)研究淋巴杉的抗炎活性。LC-MS/MS鉴定了Lymphanax-EE中的人参皂苷含量。一氧化氮(NO)测定显示了Lymphanax-EE的抗炎活性。通过定量 PCR 分析了促炎基因的表达。最后,我们通过荧光素酶分析、Western 印迹和 CETSA 确定了 lymphanax-EE 抗炎活性的内在机制。LC-MS/MS分析显示,与新鲜人参相比,淋巴-EE含有更多的原人参三醇型人参皂甙和Gyp17。Lymphanax-EE(0-200 μg/ml)可抑制LPS处理的RAW264.7细胞中NO的释放以及iNOS和COX-2等促炎细胞因子的mRNA水平。此外,lympanax-EE(200 μg/ml)还能降低NF-κB的活性以及NF-κB信号蛋白(如p65、p50、IκBα和IKKα/β)的磷酸化。最后,lympanax-EE(200 μg/ml)降低了AKT过表达诱导的IKKα/β磷酸化。在Lymphanax-EE的成分中,发现人参皂苷Re和Gyp17能抑制AKT1的活性。具有抗炎特性的人参皂苷和Gyp17通过减少NF-κB信号抑制了LPS诱导的炎症。
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Ethanol extract of lymphanax with gypenoside 17 and ginsenoside Re exerts anti-inflammatory properties by targeting the AKT/NF-κB pathway
Ginseng is processed into several types such as white ginseng, red ginseng, and black ginseng, according to the processing methods such as drying, steaming, and heating. These processing conditions can change the portion of the useful ingredients. Recently, new processing method was established to develop ‘lymphanax’, an aged fresh white ginseng prepared under anaerobic condition. This aging process was revealed to increase the content of gypenoside 17 (Gyp17) as well as ginsenoside Re, known to have anti-inflammatory effects. As the next step, therefore, we aimed to investigate the anti-inflammatory activity of lymphanax using its ethanol extract of lymphanax (Lymphanax-EE). LC-MS/MS identified the ginsenoside content of lymphanax-EE. A nitric oxide (NO) assay revealed the anti-inflammatory activity of lymphanax-EE. Pro-inflammatory gene expression was analyzed by quantitative PCR. Finally, we identified the underlying mechanism for the anti-inflammatory activity of lymphanax-EE through luciferase analysis, Western blotting, and CETSA. The LC-MS/MS analysis revealed lymphanax-EE to contain more protopanaxatriol-type ginsenosides, and Gyp17 than fresh ginseng. Lymphanax-EE (0–200 μg/ml) suppressed NO release and mRNA levels of pro-inflammatory cytokines such as iNOS and COX-2 in LPS-treated RAW264.7 cells. Moreover, lymphanax-EE (200 μg/ml) reduced the activity of NF-κB and phosphorylation of NF-κB signal proteins such as p65, p50, IκBα, and IKKα/β. Finally, lymphanax-EE (200 μg/ml) decreased the phosphorylation of IKKα/β induced by AKT overexpression. Among the components of lymphanax-EE, ginsenoside Re and Gyp17 were found to suppress AKT1 activity. Lymphanax-EE-containing ginsenosides and Gyp17 with anti-inflammatory properties suppressed LPS-induced inflammation by reducing the NF-κB signal.
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来源期刊
Journal of Ginseng Research
Journal of Ginseng Research CHEMISTRY, MEDICINAL-INTEGRATIVE & COMPLEMENTARY MEDICINE
CiteScore
11.40
自引率
9.50%
发文量
111
审稿时长
6-12 weeks
期刊介绍: Journal of Ginseng Research (JGR) is an official, open access journal of the Korean Society of Ginseng and is the only international journal publishing scholarly reports on ginseng research in the world. The journal is a bimonthly peer-reviewed publication featuring high-quality studies related to basic, pre-clinical, and clinical researches on ginseng to reflect recent progresses in ginseng research. JGR publishes papers, either experimental or theoretical, that advance our understanding of ginseng science, including plant sciences, biology, chemistry, pharmacology, toxicology, pharmacokinetics, veterinary medicine, biochemistry, manufacture, and clinical study of ginseng since 1976. It also includes the new paradigm of integrative research, covering alternative medicinal approaches. Article types considered for publication include review articles, original research articles, and brief reports. JGR helps researchers to understand mechanisms for traditional efficacy of ginseng and to put their clinical evidence together. It provides balanced information on basic science and clinical applications to researchers, manufacturers, practitioners, teachers, scholars, and medical doctors.
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