通过时间和波长分辨氧化还原生物传感器和叶绿素荧光对与电子传输相关的氧化信号进行量化

Matanel Hipsch, Nardy Lampl, Raz Lev, Shilo Rosenwasser
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引用次数: 0

摘要

通过氧化还原信号网络从光合电子链传递到目标蛋白质的还原和氧化信号是碳同化和下游代谢的关键调节因子。然而,尽管氧化还原信号在激活和抑制光合作用活性方面起着关键作用,但由于研究光合作用的传统光谱方法与氧化还原调控领域所使用的生化分析方法之间存在方法学上的差距,它们与光合作用效率的关系很难被量化。在这里,我们通过探索捕获生物传感器和叶绿素荧光信号的时间和波长分辨荧光光谱,同时量化了氧化还原信号和光合作用活性。利用一组表达基因编码的氧化还原生物传感器的马铃薯植物,我们证明了还原和氧化信号是如何随着光照强度的升高而放大的,并揭示了电子传输速率与氧化信号的产生之间的紧密联系。这些结果表明了全光谱分析如何为将基因编码生物传感器整合到光合作用研究中铺平道路,并证明了主要作物植物中抑制性氧化信号的光依赖性激活。
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Quantification of electron transport-related oxidative signals by time and wavelength-resolved redox biosensors and chlorophyll fluorescence
Reductive and oxidative signals transmitted from the photosynthetic electron chain to target proteins through the redox signaling network are key regulators of carbon assimilation and downstream metabolism. However, despite their crucial role in activating and inhibiting photosynthetic activity, their relation to photosynthetic efficiency is hardly quantified due to the methodological gap between traditional spectroscopic approaches for investigating photosynthesis and biochemical analyses used in the redox regulation field. Here, we simultaneously quantified redox signals and photosynthetic activity by exploring time and wavelength-resolved fluorescence spectra that capture biosensor and chlorophyll fluorescence signals. Using a set of potato plants expressing genetically encoded redox biosensors capable of discerning between oxidized and reduced signals, we demonstrated how reductive and oxidative signals are amplified with elevated light intensities and revealed the tight connection between electron transport rate and the generation of oxidative signals. These results demonstrate how full spectrum analysis can pave the way for the integration of genetically encoded biosensors in photosynthesis research and demonstrate light-dependent activation of inhibitory oxidative signals in major crop plants.
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