拟南芥胚珠发育过程中的核尖退化需要雌配子体的发育

IF 3.6 3区 生物学 Q1 PLANT SCIENCES Planta Pub Date : 2024-09-09 DOI:10.1007/s00425-024-04519-7
Chulmin Park, Youbong Hyun, Ji-Young Lee
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引用次数: 0

摘要

主要结论雌配子体的遗传消减为拟南芥胚珠发育过程中存在区域间交流以及雌配子体在核尖退化中的重要性提供了直接证据。被子植物的胚珠由三个区域组成:雌配子体、核和着丝粒,它们都是同步协调发育的。以前曾有人提出过区域间交流能促进胚珠的合作发育,但这些交流的证据大多依赖于对突变体表型的分析。为了提供直接证据,我们通过在雌配子体特异性 DD13 启动子下表达白喉毒素片段 A(DTA),特异性地消减了拟南芥的雌配子体,并分析了其对核仁和珠心体发育的影响。我们发现,雌配子体对珠心体的发育以及胚珠体的定向和弯曲都不是必需的,但对核尖退化却是必需的。本文介绍的结果提供了雌配子体与核尖交流的直接证据,证明拟南芥胚珠的合作发育需要区域间交流。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Female gametophyte development is required for nucellar-tip degeneration during Arabidopsis ovule development

Main conclusion

Genetic ablation of the female gametophyte provides direct evidence for the existence of interregional communication during Arabidopsis ovule development and the importance of the female gametophyte in nucellar-tip degeneration.

The angiosperm ovule consists of three regions: the female gametophyte, the nucellus, and the integuments, all of which develop synchronously and coordinately. Previously, interregional communication enabling cooperative ovule development had been proposed; however, the evidence for these communications mostly relies on the analysis of mutant phenotypes. To provide direct evidence, we specifically ablated the Arabidopsis female gametophyte by expressing the diphtheria toxin fragment A (DTA) under the female gametophyte-specific DD13 promoter and analyzed its effects on the development of the nucellus and the integuments. We found that the female gametophyte is not required for integument development or for the orientation and curvature of the ovule body, but is necessary for nucellar-tip degeneration. The results presented here provide direct evidence for communication from the female gametophyte to the nucellus and demonstrate that Arabidopsis ovules require interregional communication for cooperative development.

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来源期刊
Planta
Planta 生物-植物科学
CiteScore
7.20
自引率
2.30%
发文量
217
审稿时长
2.3 months
期刊介绍: Planta publishes timely and substantial articles on all aspects of plant biology. We welcome original research papers on any plant species. Areas of interest include biochemistry, bioenergy, biotechnology, cell biology, development, ecological and environmental physiology, growth, metabolism, morphogenesis, molecular biology, new methods, physiology, plant-microbe interactions, structural biology, and systems biology.
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