在全基因组范围内鉴定调控蒲公英天然橡胶的氧化角鲨烯环化酶基因

IF 3.6 3区 生物学 Q1 PLANT SCIENCES Planta Pub Date : 2024-09-10 DOI:10.1007/s00425-024-04522-y
Yongfei Wang, Zhanchao Yang, Boxuan Yuan, Lixia He, Yunyi Han, Juanying Wang, Xuchu Wang
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引用次数: 0

摘要

主要结论通过全基因组筛选,发现了9个TkOSC基因。摘要塔罗科植物罗丹(Taraxacum kok-saghyz Rodin,TKS)根含有大量天然橡胶、菊粉和有价值的代谢产物。氧化角鲨烯环化酶(OSC)是通过三萜类生物合成途径调节天然橡胶生物合成(NRB)的关键成员。为了探索 OSC 对 TKS 天然橡胶生产的功能,我们通过全基因组筛选在 TKS 基因组中发现了其基因家族成员。共鉴定出 9 个 TkOSC,它们主要分布在细胞质中。其家族基因在进化过程中经历了中性选择。整体序列同源性分析显示,OSC蛋白的相似度为80.23%,表明其具有高度的保守性。配对比较显示,多个序列的相似度从57%到100%不等。蛋白质相互作用预测显示,TkOSCs 可能与巴鲁醇合成酶、甾醇 1,4-脱甲基酶、羽扇豆醇合成酶和角鲨烯环氧化酶相互作用。系统进化分析表明,OSC 家族蛋白属于两个分支。TkOSC 启动子区域含有与植物生长、胁迫反应、激素反应和光反应有关的顺式作用元件。蛋白质积累分析表明,TkOSC4、TkOSC5 和 TkOSC6 蛋白在根部、乳汁和羽轴上有较高的表达水平。基因表达模式的比较表明,TkOSC1、TkOSC4、TkOSC5、TkOSC6、TkOSC7、TkOSC8 和 TkOSC9 可能在调控 NRB 中起重要作用。结合基因和蛋白质的结果表明,TkOSC4-6可能更为关键,这些数据可能有助于更深入地了解OSCs在TKS根系NRB中的作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Genome-wide identification of oxidosqualene cyclase genes regulating natural rubber in Taraxacum kok-saghyz

Main conclusion

Nine TkOSC genes have been identified by genome-wide screening. Among them, TkOSC4-6 might be more crucial for natural rubber biosynthesis in Taraxacum kok-saghyz roots.

Abstract

Taraxacum kok-saghyz Rodin (TKS) roots contain large amounts of natural rubber, inulin, and valuable metabolites. Oxidosqualene cyclase (OSC) is a key member for regulating natural rubber biosynthesis (NRB) via the triterpenoid biosynthesis pathway. To explore the functions of OSC on natural rubber producing in TKS, its gene family members were identified in TKS genome via genome-wide screening. Nine TkOSCs were identified, which were mainly distributed in the cytoplasm. Their family genes experienced a neutral selection during the evolution process. Overall sequence homology analysis OSC proteins revealed 80.23% similarity, indicating a highly degree of conservation. Pairwise comparisons showed a multiple sequence similarity ranging from 57% to 100%. Protein interaction prediction revealed that TkOSCs may interact with baruol synthase, sterol 1,4-demethylase, lupeol synthase and squalene epoxidase. Phylogenetic analysis showed that OSC family proteins belong to two branches. TkOSC promoter regions contain cis-acting elements related to plant growth, stress response, hormones response and light response. Protein accumulation analysis demonstrated that TkOSC4, TkOSC5 and TkOSC6 proteins had strong expression levels in the root, latex and plumular axis. Comparison of gene expression patterns showed TkOSC1, TkOSC4, TkOSC5, TkOSC6, TkOSC7, TkOSC8 and TkOSC9 might be important in regulating NRB. Combination of gene and protein results revealed TkOSC4-6 might be more crucial, and the data might contribute to a more profound understanding of the roles of OSCs for NRB in TKS roots.

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来源期刊
Planta
Planta 生物-植物科学
CiteScore
7.20
自引率
2.30%
发文量
217
审稿时长
2.3 months
期刊介绍: Planta publishes timely and substantial articles on all aspects of plant biology. We welcome original research papers on any plant species. Areas of interest include biochemistry, bioenergy, biotechnology, cell biology, development, ecological and environmental physiology, growth, metabolism, morphogenesis, molecular biology, new methods, physiology, plant-microbe interactions, structural biology, and systems biology.
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