{"title":"在全基因组范围内鉴定调控蒲公英天然橡胶的氧化角鲨烯环化酶基因","authors":"Yongfei Wang, Zhanchao Yang, Boxuan Yuan, Lixia He, Yunyi Han, Juanying Wang, Xuchu Wang","doi":"10.1007/s00425-024-04522-y","DOIUrl":null,"url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">\n<b><i>Main conclusion</i></b>\n</h3><p><b>Nine</b> <b><i>TkOSC</i></b> <b>genes have been identified by genome-wide screening. Among them,</b> <b><i>TkOSC4-6</i></b> <b>might be more crucial for natural rubber biosynthesis in</b> <b><i>Taraxacum kok-saghyz</i></b> <b>roots.</b></p><h3 data-test=\"abstract-sub-heading\">Abstract</h3><p><i>Taraxacum kok-saghyz</i> Rodin (TKS) roots contain large amounts of natural rubber, inulin, and valuable metabolites. Oxidosqualene cyclase (OSC) is a key member for regulating natural rubber biosynthesis (NRB) via the triterpenoid biosynthesis pathway. To explore the functions of OSC on natural rubber producing in TKS, its gene family members were identified in TKS genome via genome-wide screening. Nine <i>TkOSCs</i> were identified, which were mainly distributed in the cytoplasm. Their family genes experienced a neutral selection during the evolution process. Overall sequence homology analysis OSC proteins revealed 80.23% similarity, indicating a highly degree of conservation. Pairwise comparisons showed a multiple sequence similarity ranging from 57% to 100%. Protein interaction prediction revealed that TkOSCs may interact with baruol synthase, sterol 1,4-demethylase, lupeol synthase and squalene epoxidase. Phylogenetic analysis showed that OSC family proteins belong to two branches. <i>TkOSC</i> promoter regions contain <i>cis-</i>acting elements related to plant growth, stress response, hormones response and light response. Protein accumulation analysis demonstrated that TkOSC4, TkOSC5 and TkOSC6 proteins had strong expression levels in the root, latex and plumular axis. Comparison of gene expression patterns showed <i>TkOSC1</i>, <i>TkOSC4</i>, <i>TkOSC5</i>, <i>TkOSC6</i>, <i>TkOSC7</i>, <i>TkOSC8</i> and <i>TkOSC9</i> might be important in regulating NRB. Combination of gene and protein results revealed TkOSC4-6 might be more crucial, and the data might contribute to a more profound understanding of the roles of OSCs for NRB in TKS roots.</p>","PeriodicalId":20177,"journal":{"name":"Planta","volume":null,"pages":null},"PeriodicalIF":3.6000,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Genome-wide identification of oxidosqualene cyclase genes regulating natural rubber in Taraxacum kok-saghyz\",\"authors\":\"Yongfei Wang, Zhanchao Yang, Boxuan Yuan, Lixia He, Yunyi Han, Juanying Wang, Xuchu Wang\",\"doi\":\"10.1007/s00425-024-04522-y\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<h3 data-test=\\\"abstract-sub-heading\\\">\\n<b><i>Main conclusion</i></b>\\n</h3><p><b>Nine</b> <b><i>TkOSC</i></b> <b>genes have been identified by genome-wide screening. Among them,</b> <b><i>TkOSC4-6</i></b> <b>might be more crucial for natural rubber biosynthesis in</b> <b><i>Taraxacum kok-saghyz</i></b> <b>roots.</b></p><h3 data-test=\\\"abstract-sub-heading\\\">Abstract</h3><p><i>Taraxacum kok-saghyz</i> Rodin (TKS) roots contain large amounts of natural rubber, inulin, and valuable metabolites. Oxidosqualene cyclase (OSC) is a key member for regulating natural rubber biosynthesis (NRB) via the triterpenoid biosynthesis pathway. To explore the functions of OSC on natural rubber producing in TKS, its gene family members were identified in TKS genome via genome-wide screening. Nine <i>TkOSCs</i> were identified, which were mainly distributed in the cytoplasm. Their family genes experienced a neutral selection during the evolution process. Overall sequence homology analysis OSC proteins revealed 80.23% similarity, indicating a highly degree of conservation. Pairwise comparisons showed a multiple sequence similarity ranging from 57% to 100%. Protein interaction prediction revealed that TkOSCs may interact with baruol synthase, sterol 1,4-demethylase, lupeol synthase and squalene epoxidase. Phylogenetic analysis showed that OSC family proteins belong to two branches. <i>TkOSC</i> promoter regions contain <i>cis-</i>acting elements related to plant growth, stress response, hormones response and light response. Protein accumulation analysis demonstrated that TkOSC4, TkOSC5 and TkOSC6 proteins had strong expression levels in the root, latex and plumular axis. Comparison of gene expression patterns showed <i>TkOSC1</i>, <i>TkOSC4</i>, <i>TkOSC5</i>, <i>TkOSC6</i>, <i>TkOSC7</i>, <i>TkOSC8</i> and <i>TkOSC9</i> might be important in regulating NRB. Combination of gene and protein results revealed TkOSC4-6 might be more crucial, and the data might contribute to a more profound understanding of the roles of OSCs for NRB in TKS roots.</p>\",\"PeriodicalId\":20177,\"journal\":{\"name\":\"Planta\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.6000,\"publicationDate\":\"2024-09-10\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Planta\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1007/s00425-024-04522-y\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Planta","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s00425-024-04522-y","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
Genome-wide identification of oxidosqualene cyclase genes regulating natural rubber in Taraxacum kok-saghyz
Main conclusion
NineTkOSCgenes have been identified by genome-wide screening. Among them,TkOSC4-6might be more crucial for natural rubber biosynthesis inTaraxacum kok-saghyzroots.
Abstract
Taraxacum kok-saghyz Rodin (TKS) roots contain large amounts of natural rubber, inulin, and valuable metabolites. Oxidosqualene cyclase (OSC) is a key member for regulating natural rubber biosynthesis (NRB) via the triterpenoid biosynthesis pathway. To explore the functions of OSC on natural rubber producing in TKS, its gene family members were identified in TKS genome via genome-wide screening. Nine TkOSCs were identified, which were mainly distributed in the cytoplasm. Their family genes experienced a neutral selection during the evolution process. Overall sequence homology analysis OSC proteins revealed 80.23% similarity, indicating a highly degree of conservation. Pairwise comparisons showed a multiple sequence similarity ranging from 57% to 100%. Protein interaction prediction revealed that TkOSCs may interact with baruol synthase, sterol 1,4-demethylase, lupeol synthase and squalene epoxidase. Phylogenetic analysis showed that OSC family proteins belong to two branches. TkOSC promoter regions contain cis-acting elements related to plant growth, stress response, hormones response and light response. Protein accumulation analysis demonstrated that TkOSC4, TkOSC5 and TkOSC6 proteins had strong expression levels in the root, latex and plumular axis. Comparison of gene expression patterns showed TkOSC1, TkOSC4, TkOSC5, TkOSC6, TkOSC7, TkOSC8 and TkOSC9 might be important in regulating NRB. Combination of gene and protein results revealed TkOSC4-6 might be more crucial, and the data might contribute to a more profound understanding of the roles of OSCs for NRB in TKS roots.
期刊介绍:
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