印度根瘤菌和内圈链霉菌对水稻植物生长促进作用的生理基础

IF 4.8 1区 农林科学 Q1 AGRONOMY Rice Pub Date : 2024-09-11 DOI:10.1186/s12284-024-00732-w
Dhivya P. Thenappan, Rakesh Pandey, Alkesh Hada, Dinesh Kumar Jaiswal, Viswanathan Chinnusamy, Ramcharan Bhattacharya, Kannepalli Annapurna
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引用次数: 0

摘要

本研究证明了从水稻植株不同区域(包括根圈(FT1、FTSA2、FB2 和 FH7)和内圈(EB6))获得的本地放线菌菌株的植物生长促进能力。我们通过对植物激素通路中一组精选的关键基因进行转录分析,深入研究了这些植物-微生物相互作用产生有益影响的分子机制。通过体外筛选各种促进植物生长(PGP)的性状,所有测试的分离株都表现出吲哚-3-乙酸合成和嗜苷酸类物质生产的阳性性状,其中 FT1 是氰化氢(HCN)的唯一生产者。通过 16S rRNA 扩增,所有分离物均被鉴定为链霉菌属。在盆栽培养实验中,接种了菌株 FB2 和 FTSA2 的水稻种子的嫩枝干重分别显著增加了 7% 和 34%,总生物量分别显著增加了 8% 和 30%。所有菌株都能提高叶氮含量,其中 FTSA2 的增幅最大(4.3%)。相反,菌株 FB2 和 FT1 增加了根长、根重比、根体积和根表面积,从而提高了根氮含量。除 FB2 外,所有分离株都提高了叶绿素总量和类胡萝卜素含量。此外,qRT-PCR 分析也支持这些发现,揭示了在特定放线菌处理下,辅助素(OsAUX1、OsIAA1、OsYUCCA1、OsYUCCA3)、赤霉素(OsGID1、OsGA20ox-1)和细胞分裂素(OsIPT3、OsIPT5)通路的不同基因表达。这些放线菌株既能增强作物的地上特性,也能增强作物的地下特性,值得在田间试验中以单个菌株或联合菌株的形式进行进一步评估。这将有助于开发用于综合养分管理实践的商业生物絮凝剂。
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Physiological Basis of Plant Growth Promotion in Rice by Rhizosphere and Endosphere Associated Streptomyces Isolates from India

This study demonstrated the plant growth-promoting capabilities of native actinobacterial strains obtained from different regions of the rice plant, including the rhizosphere (FT1, FTSA2, FB2, and FH7) and endosphere (EB6). We delved into the molecular mechanisms underlying the beneficial effects of these plant-microbe interactions by conducting a transcriptional analysis of a select group of key genes involved in phytohormone pathways. Through in vitro screening for various plant growth-promoting (PGP) traits, all tested isolates exhibited positive traits for indole-3-acetic acid synthesis and siderophore production, with FT1 being the sole producer of hydrogen cyanide (HCN). All isolates were identified as members of the Streptomyces genus through 16S rRNA amplification. In pot culture experiments, rice seeds inoculated with strains FB2 and FTSA2 exhibited significant increases in shoot dry mass by 7% and 34%, respectively, and total biomass by 8% and 30%, respectively. All strains led to increased leaf nitrogen levels, with FTSA2 demonstrating the highest increase (4.3%). On the contrary, strains FB2 and FT1 increased root length, root weight ratio, root volume, and root surface area, leading to higher root nitrogen content. All isolates, except for FB2, enhanced total chlorophyll and carotenoid levels. Additionally, qRT-PCR analysis supported these findings, revealing differential gene expression in auxin (OsAUX1, OsIAA1, OsYUCCA1, OsYUCCA3), gibberellin (OsGID1, OsGA20ox-1), and cytokinin (OsIPT3, OsIPT5) pathways in response to specific actinobacterial treatments. These actinobacterial strains, which enhance both aboveground and belowground crop characteristics, warrant further evaluation in field trials, either as individual strains or in consortia. This could lead to the development of commercial bioinoculants for use in integrated nutrient management practices.

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来源期刊
Rice
Rice AGRONOMY-
CiteScore
10.10
自引率
3.60%
发文量
60
审稿时长
>12 weeks
期刊介绍: Rice aims to fill a glaring void in basic and applied plant science journal publishing. This journal is the world''s only high-quality serial publication for reporting current advances in rice genetics, structural and functional genomics, comparative genomics, molecular biology and physiology, molecular breeding and comparative biology. Rice welcomes review articles and original papers in all of the aforementioned areas and serves as the primary source of newly published information for researchers and students in rice and related research.
期刊最新文献
OsPIPK-FAB, A Negative Regulator in Rice Immunity Unveiled by OsMBL1 Inhibition. CRISPR-Based Modulation of uORFs in DEP1 and GIF1 for Enhanced Rice Yield Traits. Indole-3-Acetic Acid (IAA) and Sugar Mediate Endosperm Development in Rice (Oryza sativa L.). OsPP2C49, a Negative Regulatory Factor in the Abscisic Acid Signaling Pathway, Positively Regulates Grain Yield in Rice. A Cyclin Gene OsCYCB1;5 Regulates Seed Callus Induction in Rice Revealed by Genome Wide Association Study.
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