揭示大豆对早期和晚期四膜虫(Acari: Tetranychidae)侵袭的反应

IF 4.2 3区 生物学 Q1 PLANT SCIENCES Plant Biology Pub Date : 2024-09-09 DOI:10.1111/plb.13717
K. Ruffatto, L. C. O. da Silva, C. d. O. Neves, S. G. Kuntzler, J. C. de Lima, F. A. Almeida, V. Silveira, F. M. Corrêa, L. V. P. Minello, L. Johann, R. A. Sperotto
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引用次数: 0

摘要

大豆是全球重要的粮食、蛋白质和油料来源,它正面临着来自生物胁迫的挑战。Tetranychus urticae Koch(Acari:Tetranychidae)的侵染对植物生长和谷物产量的影响尤为严重。了解大豆对 T. urticae 侵染的反应对于揭示螨虫-植物相互作用的动态至关重要。我们评估了大豆植株在螨虫侵染 5 天和 21 天后的生理和分子反应。我们采用肉眼/显微镜观察叶片损伤、H2O2 积累和脂质过氧化反应。此外,我们还分析了螨虫侵袭对芽长/干重、叶绿素浓度和发育阶段的影响。蛋白质组分析确定了螨虫侵染早期(5 天)和晚期(21 天)的不同丰度蛋白质(DAPs)。此外,还进行了 GO、KEGG 和蛋白质相互作用分析,以了解对代谢途径的影响。在整个分析期间,叶片损伤症状、H2O2 积累和脂质过氧化持续增加。螨虫侵染降低了芽的长度/干重、叶绿素浓度和发育阶段的持续时间。蛋白质组学研究发现,螨虫侵染早期和晚期的发育阶段持续时间分别为 185 天和 266 天,表明新陈代谢途径发生了复杂的重塑。光蒸腾、叶绿素合成、氨基酸代谢和克雷布斯循环/能量产生在早期和晚期侵染后都受到了影响。此外,只有在早期或晚期侵染后,特定的代谢途径才会发生改变。这项研究强调了螨虫侵染对大豆生理和代谢的不利影响。DAPs 为增强抗性的育种计划提供了潜力。总之,这项研究强调了大豆对螨虫侵染反应的复杂性,为干预和育种策略提供了启示。
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Unravelling soybean responses to early and late Tetranychus urticae (Acari: Tetranychidae) infestation

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来源期刊
Plant Biology
Plant Biology 生物-植物科学
CiteScore
8.20
自引率
2.60%
发文量
109
审稿时长
3 months
期刊介绍: Plant Biology is an international journal of broad scope bringing together the different subdisciplines, such as physiology, molecular biology, cell biology, development, genetics, systematics, ecology, evolution, ecophysiology, plant-microbe interactions, and mycology. Plant Biology publishes original problem-oriented full-length research papers, short research papers, and review articles. Discussion of hot topics and provocative opinion articles are published under the heading Acute Views. From a multidisciplinary perspective, Plant Biology will provide a platform for publication, information and debate, encompassing all areas which fall within the scope of plant science.
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