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引用次数: 0
摘要
一直以来，大多数昆虫时间生态学研究都使用直接观察、照相机或基于红外光束的监测器来量化定时间隔内的运动。虽然一些 DIY 替代系统比目前的标准运动活动监测器便宜，但这些方案的构建仍然复杂和/或计算密集。我们开发了成像运动活动监测器（iLAM），这是一种经济实惠（约 75 美元/台）的活动量化系统。iLAM 利用飞行笼内的 Raspberry Pi Zero W 计算机和夜视摄像机，以用户定义的时间间隔拍摄昆虫群体。开源的模块化 R 脚本处理图像，并输出一个文件，其中包含连续图像之间识别出的所有运动（blobs）的数量、大小、坐标位置和时间戳。输出结果可以直接进行分析，也可以转换成标准的 TriKinetics DAM 格式。我们通过比较不同昆虫物种（萤火虫：Photinus marginellus、P. greeni、P. obscurellus）、生态型（蛾：Ostrinia nubilalis）和性别（蛾：O. nubilalis）的昼夜活动，展示了 iLAM 系统的灵活性和强大功能。只有六台 iLAM（450 美元）采集到的数据表明，O. nubilalis 雌蛾的活动高峰期（AZT：19.2 小时）明显早于雄蛾（22.0 小时）。此外，来自单基因种群的雄蛾的内源期长度（AZT：21.3 小时）明显短于来自双基因背景的雄蛾（22.7 小时）。iLAM将成为研究人员测量不同物种、性别和种群在恒定和变化环境中的运动活动的重要工具。
期刊介绍：
A British Ecological Society journal, Methods in Ecology and Evolution (MEE) promotes the development of new methods in ecology and evolution, and facilitates their dissemination and uptake by the research community. MEE brings together papers from previously disparate sub-disciplines to provide a single forum for tracking methodological developments in all areas.
MEE publishes methodological papers in any area of ecology and evolution, including:
-Phylogenetic analysis
-Statistical methods
-Conservation & management
-Theoretical methods
-Practical methods, including lab and field
-This list is not exhaustive, and we welcome enquiries about possible submissions. Methods are defined in the widest terms and may be analytical, practical or conceptual.
A primary aim of the journal is to maximise the uptake of techniques by the community. We recognise that a major stumbling block in the uptake and application of new methods is the accessibility of methods. For example, users may need computer code, example applications or demonstrations of methods.
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