Bastien Cayrol, Irene Arnoldi, Vladimir Novak, Sara Epis, Matteo Brilli, Yvan Rahbé, Marilyne Uzest, Paolo Gabrieli
{"title":"同步辐射 X 射线显微层析技术揭示的传播疾病的蚜虫和蚊子最后一次蜕皮期间穿刺口的发育过程","authors":"Bastien Cayrol, Irene Arnoldi, Vladimir Novak, Sara Epis, Matteo Brilli, Yvan Rahbé, Marilyne Uzest, Paolo Gabrieli","doi":"10.1127/entomologia/2024/2475","DOIUrl":null,"url":null,"abstract":"Piercing-sucking insects are extremely efficient vectors of animal and plant pathogens. This group is polyphyletic with the piercing organ highly heterogenous in its structure and morphogenesis, adapted to a specific host and feeding mechanism. For instance, hemimetabolous aphids feed on plant sap from nymphs to adults and they renew their stylets at each molt, thanks to specialized secreting glands. Distinctly, holometabolous mosquitoes have two feeding modes: larvae feed in water by filtering and scraping surfaces with their mouths, while female adults can acquire blood from vertebrate hosts. The pupal metamorphosis allows switching from one feeding habit to another. Here, we present a deep characterization of the biogenesis of the adult mouthparts in parthenogenetic females of the pea aphid Acyrthosiphon pisum (Harris 1776) (Hemiptera: Aphididae) and in females of the mosquito Aedes albopictus (Skuse 1895) (Diptera: Culicidae), investigated using non-invasive X-ray synchrotron-based microtomography. Comparing datasets collected from aphid juvenile and adult stages and from preimaginal and adult stages of the mosquito, we were able to track the morphological changes of secreting glands and the synthesis of the adult stylet in aphid heads and to follow the de novo formation of mosquito mouthparts in pupae. Our study provides a baseline for investigating the evolution and the development of piercing-sucking mouthparts and to better understand how morphogenesis works in insects.","PeriodicalId":11728,"journal":{"name":"Entomologia Generalis","volume":null,"pages":null},"PeriodicalIF":5.6000,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The development of the piercing mouth during the last molt of the diseases-transmitting aphids and mosquitoes as revealed by synchrotron X-ray microtomography\",\"authors\":\"Bastien Cayrol, Irene Arnoldi, Vladimir Novak, Sara Epis, Matteo Brilli, Yvan Rahbé, Marilyne Uzest, Paolo Gabrieli\",\"doi\":\"10.1127/entomologia/2024/2475\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Piercing-sucking insects are extremely efficient vectors of animal and plant pathogens. This group is polyphyletic with the piercing organ highly heterogenous in its structure and morphogenesis, adapted to a specific host and feeding mechanism. For instance, hemimetabolous aphids feed on plant sap from nymphs to adults and they renew their stylets at each molt, thanks to specialized secreting glands. Distinctly, holometabolous mosquitoes have two feeding modes: larvae feed in water by filtering and scraping surfaces with their mouths, while female adults can acquire blood from vertebrate hosts. The pupal metamorphosis allows switching from one feeding habit to another. Here, we present a deep characterization of the biogenesis of the adult mouthparts in parthenogenetic females of the pea aphid Acyrthosiphon pisum (Harris 1776) (Hemiptera: Aphididae) and in females of the mosquito Aedes albopictus (Skuse 1895) (Diptera: Culicidae), investigated using non-invasive X-ray synchrotron-based microtomography. Comparing datasets collected from aphid juvenile and adult stages and from preimaginal and adult stages of the mosquito, we were able to track the morphological changes of secreting glands and the synthesis of the adult stylet in aphid heads and to follow the de novo formation of mosquito mouthparts in pupae. Our study provides a baseline for investigating the evolution and the development of piercing-sucking mouthparts and to better understand how morphogenesis works in insects.\",\"PeriodicalId\":11728,\"journal\":{\"name\":\"Entomologia Generalis\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":5.6000,\"publicationDate\":\"2024-09-05\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Entomologia Generalis\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1127/entomologia/2024/2475\",\"RegionNum\":1,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ENTOMOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Entomologia Generalis","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1127/entomologia/2024/2475","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENTOMOLOGY","Score":null,"Total":0}
The development of the piercing mouth during the last molt of the diseases-transmitting aphids and mosquitoes as revealed by synchrotron X-ray microtomography
Piercing-sucking insects are extremely efficient vectors of animal and plant pathogens. This group is polyphyletic with the piercing organ highly heterogenous in its structure and morphogenesis, adapted to a specific host and feeding mechanism. For instance, hemimetabolous aphids feed on plant sap from nymphs to adults and they renew their stylets at each molt, thanks to specialized secreting glands. Distinctly, holometabolous mosquitoes have two feeding modes: larvae feed in water by filtering and scraping surfaces with their mouths, while female adults can acquire blood from vertebrate hosts. The pupal metamorphosis allows switching from one feeding habit to another. Here, we present a deep characterization of the biogenesis of the adult mouthparts in parthenogenetic females of the pea aphid Acyrthosiphon pisum (Harris 1776) (Hemiptera: Aphididae) and in females of the mosquito Aedes albopictus (Skuse 1895) (Diptera: Culicidae), investigated using non-invasive X-ray synchrotron-based microtomography. Comparing datasets collected from aphid juvenile and adult stages and from preimaginal and adult stages of the mosquito, we were able to track the morphological changes of secreting glands and the synthesis of the adult stylet in aphid heads and to follow the de novo formation of mosquito mouthparts in pupae. Our study provides a baseline for investigating the evolution and the development of piercing-sucking mouthparts and to better understand how morphogenesis works in insects.
期刊介绍:
Its scope covers all aspects of basic and applied research dealing with insects and more broadly with arthropods inhabiting wild, agricultural and/or urban habitats. The journal also considers research integrating various disciplines and issues within the broad field of entomology and ecology.
Entomologia Generalis publishes high quality research articles on advances in knowledge on the ecology and biology of arthropods, as well as on their importance for key ecosystems services, e.g. as biological control and pollination. The journal devotes special attention to contributions providing significant advances (i) on the fundamental knowledge and on sustainable control strategies of arthropod pests (including of stored products) and vectors of diseases, (ii) on the biology and ecology of beneficial arthropods, (iii) on the spread and impact of invasive pests, and (iv) on potential side effects of pest management methods.
Entomologia Generalis welcomes review articles on significant developments in the field of entomology. These are usually invited by the editorial board, but proposals may be sent to the Editor-in-Chief for preliminary assessment by the editorial board before formal submission to the journal. The journal also considers comments on papers published in Entomologia Generalis, as well as short notes on topics that are of broader interest.