Kai Wu, Kai Li, Weihan Jia, Kathleen R. Stoof-Leichsenring, Ulrike Herzschuh, Jian Ni, Mengna Liao, Fang Tian
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The applicability and limitations of using plant DNA metabarcoding for modern vegetation monitoring and palaeo-vegetation reconstructions have been assessed by comparing sedDNA, pollen, and vegetation survey data. Our results showed that plant DNA metabarcoding recorded 186 terrestrial taxa, of which 30.1% can be identified at the species level. The plant sedDNA approach can effectively disclose the dominant plant taxa (including Asteraceae, Cyperaceae and Poaceae) and significant vegetation assemblages in the vicinity of the investigated sites. The number of taxa and taxonomic resolution of plant sedDNA exceeded that of pollen analysis (75 taxa detected, 5.3% can be identified at species level). Unlike pollen that retains a broad spectrum of regional plant signals (including <i>Pinus</i> and <i>Artemisia</i>), plant sedDNA mirrors very local plants, underscoring its utility in local vegetation monitoring and reconstructions. To conclude, plant DNA metabarcoding of (small) lake sediments warrant increased attention in the future for local vegetation monitoring and reconstructions on the TP.</p>","PeriodicalId":21651,"journal":{"name":"Science China Earth Sciences","volume":null,"pages":null},"PeriodicalIF":6.0000,"publicationDate":"2024-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Application of plant DNA metabarcoding of lake sediments for monitoring vegetation compositions on the Tibetan Plateau\",\"authors\":\"Kai Wu, Kai Li, Weihan Jia, Kathleen R. Stoof-Leichsenring, Ulrike Herzschuh, Jian Ni, Mengna Liao, Fang Tian\",\"doi\":\"10.1007/s11430-023-1358-0\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Benefiting from the rapid development of environmental DNA (eDNA) technologies, sedimentary DNA (sedDNA) emerges as a promising tool for monitoring plant compositions in remote regions. 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The number of taxa and taxonomic resolution of plant sedDNA exceeded that of pollen analysis (75 taxa detected, 5.3% can be identified at species level). Unlike pollen that retains a broad spectrum of regional plant signals (including <i>Pinus</i> and <i>Artemisia</i>), plant sedDNA mirrors very local plants, underscoring its utility in local vegetation monitoring and reconstructions. 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引用次数: 0
摘要
得益于环境 DNA(eDNA)技术的飞速发展,沉积 DNA(sedDNA)成为监测偏远地区植物组成的一种前景广阔的工具。青藏高原(TP)以环境恶劣、池塘湖泊众多而闻名,是应用沉积 DNA 进行植被调查的潜在高要求地区。在此,我们使用叶绿体trnL(UAA)内含子P6环区的g和h通用引物扩增了青藏高原西南部59个池塘和小湖泊表层沉积物中的植物DNA。通过比较沉积物 DNA、花粉和植被调查数据,评估了使用植物 DNA 代谢编码进行现代植被监测和古植被重建的适用性和局限性。结果表明,植物 DNA 元标定记录了 186 个陆生类群,其中 30.1%可在物种水平上进行鉴定。植物沉积 DNA 方法可有效揭示调查地点附近的主要植物类群(包括菊科、鹅掌楸科和罂粟科)和重要植被组合。植物沉积 DNA 的类群数量和分类分辨率都超过了花粉分析(检测到 75 个类群,5.3% 可确定物种级别)。花粉保留了广泛的区域性植物信号(包括松树和蒿草),而植物沉积 DNA 则不同,它反映了非常局部的植物,突出了其在局部植被监测和重建中的实用性。总之,(小型)湖泊沉积物中的植物 DNA 代谢标定值得在未来进一步关注,以用于对当地植被进行监测和重建。
Application of plant DNA metabarcoding of lake sediments for monitoring vegetation compositions on the Tibetan Plateau
Benefiting from the rapid development of environmental DNA (eDNA) technologies, sedimentary DNA (sedDNA) emerges as a promising tool for monitoring plant compositions in remote regions. The Tibetan Plateau (TP), renowned for its harsh environment and numerous ponds and lakes, presents a potentially demanding region for the application of sedDNA on vegetation investigations. Here, we used the g and h universal primers for the P6 loop region of the chloroplast trnL (UAA) intron to amplify plant DNA in surface sediments from 59 ponds and small lakes on the southwestern TP. The applicability and limitations of using plant DNA metabarcoding for modern vegetation monitoring and palaeo-vegetation reconstructions have been assessed by comparing sedDNA, pollen, and vegetation survey data. Our results showed that plant DNA metabarcoding recorded 186 terrestrial taxa, of which 30.1% can be identified at the species level. The plant sedDNA approach can effectively disclose the dominant plant taxa (including Asteraceae, Cyperaceae and Poaceae) and significant vegetation assemblages in the vicinity of the investigated sites. The number of taxa and taxonomic resolution of plant sedDNA exceeded that of pollen analysis (75 taxa detected, 5.3% can be identified at species level). Unlike pollen that retains a broad spectrum of regional plant signals (including Pinus and Artemisia), plant sedDNA mirrors very local plants, underscoring its utility in local vegetation monitoring and reconstructions. To conclude, plant DNA metabarcoding of (small) lake sediments warrant increased attention in the future for local vegetation monitoring and reconstructions on the TP.
期刊介绍:
Science China Earth Sciences, an academic journal cosponsored by the Chinese Academy of Sciences and the National Natural Science Foundation of China, and published by Science China Press, is committed to publishing high-quality, original results in both basic and applied research.