利用体外实验评估 PEG 化铂纳米粒子对人类乳腺癌细胞系的抗癌特性。

IF 1.3 Q3 RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING Biomedical Physics & Engineering Express Pub Date : 2024-09-11 DOI:10.1088/2057-1976/ad795d
Felicia Aswathy W,Jiya Jose,E I Anila
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引用次数: 0

摘要

本研究描述了 PEG-400(聚乙二醇-400)包裹的铂纳米粒子(PEGylated Pt NPs)对正常细胞系和癌细胞系的体外细胞毒性作用。使用 X 射线衍射和拉曼光谱进行了结构表征,平均结晶尺寸为 5.7 nm,使用扫描电子显微镜(SEM)进行的形态评估显示存在球形铂纳米粒子。能量色散 X 射线光谱(EDX)的结果表明,按重量计,铂含量与碳和氧的百分比较高,而碳和氧是封端剂的预期成分,这证实了铂样品的纯度。动态光散射实验显示,PEG 化铂 NPs 的平均流体力学直径为 353.6 nm。利用 3-(4, 5-二甲基噻唑-2-基)-2, 5-二苯基溴化四氮唑(MTT)检测法评估了 PEG 化铂 NPs 对正常细胞系(L929)和乳腺癌细胞系(MCF-7)的细胞毒性。结果显示,对癌细胞株的 IC50 值为 79.18 µg/ml,而对正常细胞株则无毒性。在双重染色凋亡试验中,观察到随着 PEG 化铂纳米粒子样品浓度的增加,与铂纳米粒子一起培养的细胞死亡率增加,MCF-7 细胞的增殖活性逐渐减弱。体外 DCFH-DA 分析法评估了纳米颗粒处理细胞的氧化应激,揭示了 PEG 化铂纳米颗粒抗癌活性的机理背景是 ROS 辅助线粒体功能障碍。
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Assessing Anticancer Properties of PEGylated Platinum Nanoparticles on Human Breast Cancer Cell lines using in-vitro Assays.
This study describes the in-vitro cytotoxic effects of PEG-400 (Polyethylene glycol-400)-capped platinum nanoparticles (PEGylated Pt NPs) on both normal and cancer cell lines. Structural characterization was carried out using X-ray diffraction and Raman spectroscopy with an average crystallite size 5.7 nm, and morphological assessment using Scanning electron microscopy (SEM) revealed the presence of spherical platinum nanoparticles. The results of energy-dispersive X-ray spectroscopy (EDX) showed a higher percentage fraction of platinum content by weight, along with carbon and oxygen, which are expected from the capping agent, confirming the purity of the platinum sample. The dynamic light scattering experiment revealed an average hydrodynamic diameter of 353.6 nm for the PEGylated Pt NPs. The cytotoxicity profile of PEGylated Pt NPs was assessed on a normal cell line (L929) and a breast cancer cell line (MCF-7) using the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. The results revealed an IC50 of 79.18 µg/ml on the cancer cell line and non-toxic behaviour on the normal cell line. In the dual staining apoptosis assay, it was observed that the mortality of cells cultured in conjunction with platinum nanoparticles intensified and the proliferative activity of MCF-7 cells gradually diminished over time in correlation with the increasing concentration of the PEGylated Pt NPs sample. The in vitro DCFH-DA assay for oxidative stress assessment in nanoparticle-treated cells unveiled the mechanistic background of the anticancer activity of PEGylated platinum nanoparticles as ROS-assisted mitochondrial dysfunction.
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来源期刊
Biomedical Physics & Engineering Express
Biomedical Physics & Engineering Express RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING-
CiteScore
2.80
自引率
0.00%
发文量
153
期刊介绍: BPEX is an inclusive, international, multidisciplinary journal devoted to publishing new research on any application of physics and/or engineering in medicine and/or biology. Characterized by a broad geographical coverage and a fast-track peer-review process, relevant topics include all aspects of biophysics, medical physics and biomedical engineering. Papers that are almost entirely clinical or biological in their focus are not suitable. The journal has an emphasis on publishing interdisciplinary work and bringing research fields together, encompassing experimental, theoretical and computational work.
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