Biomedical Physics & Engineering Express最新文献

[Introduction] Monte Carlo simulation codes simulating medical imaging nuclear detectors (SIMIND) are notable tools used to model nuclear medicine experiments.This study aimed to confirm the usability of SIMIND as an alternative method for nuclear medicine experiments with a cardiac phantom HL, simulating human body structures, by comparing the actual experiment data. [Methods] A cardiac phantom HL that simulates myocardial scintigraphy using ¹²³I-meta-iodobenzylguanidine was developed, and single-photon emission computed tomography/computed tomography imaging was performed using Discovery NM/CT 670 scanner. Aside from the main-energy window(159 keV ± 10%), additional windows were set on the low(137.5 keV ± 4% ) and high(180.5 keV ± 3%)-energy sides. The simulations were performed under the same conditions as the actual experiments. Regions of interest (ROIs) were set in each organ part of the experiments and simulated data, and a polar map for the myocardial part was developed. The mean, maximum (max), and minimum (min) counts within each ROI, as well as the relative errors of each segment in the polar map, were calculated to evaluate the accuracy of the simulation. [Results] Overall, the results were favorable with relative errors of <10% except in some areas based on the data from the main-energy window and postreconstruction. On the other hand, relative errors of ＞10% were found in both the low and high subenergy windows. The smallest error occurred when assessing using mean values within the ROIs. The relative error was high at the cardiac base in the polar map evaluation; however, it remained <10% from the mid to apical heart sections. [Conclusion] SIMIND is considered an alternative method for nuclear medicine experiments using a myocardial phantom HL that closely resembles human body structures. However, caution is warranted as accuracy may decrease under specific conditions.

Objective. To develop a robust method for non-contact surface dosimetry during Total Body Irradiation (TBI) that uses an optimally paired choice of scintillator material with camera photocathode and can work insensitively to the normal ambient room lighting conditions (∼500 Lux).Approach. This goal was approached by assessing the emission contrast of scintillator signal to background room ratio (SBR) detected by the camera, in the challening conditions of low dose rate TBI with high room lights. A total of 9 fast-response scintillators, 3 wavelength shifters, and 2 camera photocathodes were systematically tested to determine the optimal combination. The effects of room lights on the scintillator signal and the background signal were assessed to avoid signal saturation while retaining accurate dose measurement. A bandpass wavelength filter was then applied to reduce the effects on room lights and scintillator signal.Main Results. One scintillator (EJ262) combined with a blue-green sensitive photocathode camera and a 500 nm band pass filter produced the greatest available scintillator SBR of 95 with maximal room lights on. The caveat is that this design rejects all patient Cherenkov light, which can be useful for visualizing the patient treatment. Another option which retained the Cherenkov signal but produced less available scintillator signal was found with another scintillator (EJ-260) and a red photocathode camera with SBR of 35, but a narrow bandpass filter is required to make it work in ambient room lights, which addition will also remove most of the Cherenkov signal.Significance. Non-contact scintillator imaging can be used for surface dosimetry in TBI with appropriate pairing of scintillator emission spectrum and camera photocathode sensitivity or optical filtering range.

Colorectal cancer (CRC) remains a leading cause of cancer-related deaths worldwide, necessitating the development of novel therapeutic approaches. Carbon dots (CDs) have emerged as promising nanoparticles for biomedical applications due to their unique properties. Gallic acid (GA), an anticancer agent, is effective against various tumor types. This study explores the potential of gallic acid-derived carbon dots (GA-CDs) as an innovative anticancer agent against HCT-116 CRC cells, focusing on apoptosis signaling pathways. GA-CDs were synthesized using a one-pot hydrothermal method. Characterization was conducted using transmission electron microscopy (TEM), Fourier transform infrared (FT-IR) spectroscopy, and ultraviolet-visible (UV-vis) absorption spectroscopy. The cytotoxicity of GA and GA-CDs on HCT-116 cells was evaluated using the MTT assay at various concentrations over 24 and 48 h. Cellular uptake was assessed via fluorescence microscopy, and apoptosis was analyzed using acridine orange/propidium iodide (AO/PI) staining. Total RNA extraction followed by complementary DNA (cDNA) synthesis via reverse transcription-PCR was performed, and real time-PCR (Q-PCR) was conducted to examine the expression of apoptosis-related genes includingCaspase-3,Bax, andBcl-2. Characterization confirmed the successful synthesis of spherical GA-CDs. GA-CDs exhibited dose- and time-dependent cytotoxicity, with IC50 values of 88.55 μg ml^-1for GA-CDs and 192.2 μg ml^-1for GA after 24 h. Fluorescence microscopy confirmed the efficient uptake of GA-CDs by HCT-116 cells. AO/PI staining showed a significant increase in apoptotic cell numbers after treatment with GA-CDs. Q-PCR analysis revealed overexpression ofCaspase-3 andBaxgenes in GA-CD-treated cells, though no significant changes were observed in the expression ofBcl-2 or theBax/Bcl-2 ratio. GA-CDs demonstrated potent anticancer properties by inducing apoptosis and reducing cell viability in HCT-116 cells. These findings suggest the potential of GA-CDs as a novel therapeutic agent for CRC treatment, warranting further investigation into their mechanism of action andin vivoefficacy.

Objectivea previous study reported nanodosimetric measurements of therapeutic-energy carbon ions penetrating simulated tissue. The results are incompatible with the predicted mean energy of the carbon ions in the nanodosimeter and previous experiments with lower energy monoenergetic beams. The purpose of this study is to explore the origin of these discrepancies.Approachdetailed simulations using the Geant4 toolkit were performed to investigate the radiation field in the nanodosimeter and provide input data for track structure simulations, which were performed with a developed version of the PTra code.Main resultsthe Geant4 simulations show that with the narrow-beam geometry employed in the experiment, only a small fraction of the carbon ions traverse the nanodosimeter and their mean energy is between 12% and 30% lower than the values estimated using the SRIM software. Only about one-third or less of these carbon ions hit the trigger detector. The track structure simulations indicate that the observed enhanced ionization cluster sizes are mainly due to coincidences with events in which carbon ions miss the trigger detector. In addition, the discrepancies observed for high absorber thicknesses of carbon ions traversing the target volume could be explained by assuming an increase in thickness or interaction cross-sections in the order of 1%.Significancethe results show that even with strong collimation of the radiation field, future nanodosimetric measurements of clinical carbon ion beams will require large trigger detectors to register all events with carbon ions traversing the nanodosimeter. Energy loss calculations of the primary beam in the absorbers are insufficient and should be replaced by detailed simulations when planning such experiments. Uncertainties of the interaction cross-sections in simulation codes may shift the Bragg peak position.

Retinopathy of Prematurity (ROP) is a retinal disorder affecting preterm babies, which can lead to permanent blindness without treatment. Early-stage ROP diagnosis is vital in providing optimal therapy for the neonates. The proposed study predicts early-stage ROP from neonatal fundus images using Machine Learning (ML) classifiers and Convolutional Neural Networks (CNN) based pre-trained networks. The characteristic demarcation lines and ridges in early stage ROP are segmented utilising a novel Swin U-Net. 2000 Scale Invariant Feature Transform (SIFT) descriptors were extracted from the segmented ridges and are dimensionally reduced to 50 features using Principal Component Analysis (PCA). Seven ROP-specific features, including six Gray Level Co-occurrence Matrix (GLCM) and ridge length features, are extracted from the segmented image and are fused with the PCA reduced 50 SIFT features. Finally, three ML classifiers, such as Support Vector Machine (SVM), Random Forest (RF), andk- Nearest Neighbor (k-NN), are used to classify the 50 features to predict the early-stage ROP from Normal images. On the other hand, the raw retinal images are classified directly into normal and early-stage ROP using six pre-trained classifiers, namely ResNet50, ShuffleNet V2, EfficientNet, MobileNet, VGG16, and DarkNet19. It is seen that the ResNet50 network outperformed all other networks in predicting early-stage ROP with 89.5% accuracy, 87.5% sensitivity, 91.5% specificity, 91.1% precision, 88% NPV and an Area Under the Curve (AUC) of 0.92. Swin U-Net Convolutional Neural Networks (CNN) segmented the ridges and demarcation lines with an accuracy of 89.7% with 80.5% precision, 92.6% recall, 75.76% IoU, and 0.86 as the Dice coefficient. The SVM classifier using the 57 features from the segmented images achieved a classification accuracy of 88.75%, sensitivity of 90%, specificity of 87.5%, and an AUC of 0.91. The system can be utilised as a point-of-care diagnostic tool for ROP diagnosis of neonates in remote areas.

Lung cancer is one of the most common life-threatening worldwide cancers affecting both the male and the female populations. The appearance of nodules in the scan image is an early indication of the development of cancer cells in the lung. The Low Dose Computed Tomography screening technique is used for the early detection of cancer nodules. Therefore, with more Computed Tomography (CT) lung profiles, an automated lung nodule analysis system can be utilized through image processing techniques and neural network algorithms. A CT image of the lung consists of many elements such as blood vessels, ribs, nodules, sternum, bronchi and nodules. These nodules can be both benign and malignant, where the latter leads to lung cancer. Detecting them at an earlier stage can increase life expectancy by up to 5 to 10 years. To analyse only the nodules from the profile, the respected features are extracted using image processing techniques. Based on the review, textural features were the promising ones in medical image analysis and for solving computer vision problems. The importance of uncovering the hidden features allows Deep Learning algorithms (DL) to function better, especially in medical imaging, where accuracy has improved. The earlier detection of cancerous lung nodules is possible through the combination of multi-featured extraction and classification techniques using image data. This technique can be a breakthrough in the deep learning area by providing the appropriate features. One of the greatest challenges is the incorrect identification of malignant nodules results in a higher false positive rate during the prediction. The suitable features make the system more precise in prognosis. In this paper, the overview of lung cancer along with the publicly available datasets is discussed for the research purposes. They are mainly focused on the recent research that combines feature extraction and deep learning algorithms used to reduce the false positive rate in the automated detection of lung nodules. The primary objective of the paper is to provide the importance of textural features when combined with different deep-learning models. It gives insights into their advantages, disadvantages and limitations regarding possible research gaps. These papers compare the recent studies of deep learning models with and without feature extraction and conclude that DL models that include feature extraction are better than the others.

Orthopedic injuries, such as femur shaft fractures, often require surgical intervention to promote healing and functional recovery. Metal plate implants are widely used due to their mechanical strength and biocompatibility. Biodegradable metal plate implants, including those made from magnesium, zinc, and iron alloys, offer distinct advantages over non-biodegradable materials like stainless steel, titanium, and cobalt alloys. Biodegradable implants gradually replace native bone tissue, reducing the need for additional surgeries and improving patient recovery. However, non-biodegradable implants remain popular due to their stability, corrosion resistance, and biocompatibility. This study focuses on designing an implant plate for treating transverse femoral shaft fractures during the walking cycle. The primary objective is to conduct a comprehensive finite element analysis (FEA) of a fractured femur's stabilization using various biodegradable and non-biodegradable materials. The study assesses the efficacy of different implant materials, discusses implant design, and identifies the optimal materials for femoral stabilization. Results indicate that magnesium alloy is superior among biodegradable materials, while titanium alloy is preferred among non-biodegradable options. The findings suggest that magnesium alloy is the recommended material for bone implants due to its advantages over non-degradable alternatives.

Due to the inherent variability in EEG signals across different individuals, domain adaptation and adversarial learning strategies are being progressively utilized to develop subject-specific classification models by leveraging data from other subjects. These approaches primarily focus on domain alignment and tend to overlook the critical task-specific class boundaries. This oversight can result in weak correlation between the extracted features and categories. To address these challenges, we propose a novel model that uses the known information from multiple subjects to bolster EEG classification for an individual subject through adversarial learning strategies. Our method begins by extracting both shallow and attention-driven deep features from EEG signals. Subsequently, we employ a class discriminator to encourage the same-class features from different domains to converge while ensuring that the different-class features diverge. This is achieved using our proposed discrimination loss function, which is designed to minimize the feature distance for samples of the same class across different domains while maximizing it for those from different classes. Additionally, our model incorporates two parallel classifiers that are harmonious yet distinct and jointly contribute to decision-making. Extensive testing on two publicly available EEG datasets has validated our model's efficacy and superiority.

Purpose. This study aimed to develop a new method for automated contrast-to-noise ratio (CNR) measurement using the low-contrast object in the ACR computed tomography (CT) phantom.Methods. The proposed method for CNR measurement was based on statistical criteria. A region of interest (ROI) was placed in a specific radial location and was then rotated around 360° in increments of 2°. At each position, the average CT number within the ROI was calculated. After one complete rotation, a profile of the average CT number around the full rotation was obtained. The center coordinate of the low-contrast object was determined from the maximum value of the profile. The CNR was calculated based on the average CT number and noise within the ROI in the low-contrast object and the ROI in the background, i.e., at the center of the phantom. The proposed method was used to evaluate CNR from images scanned with various phantom rotations, images with various noise levels (tube currents), and images from 25 CT scanners. The results were compared to a previous method based on a threshold approach.Results. The proposed method successfully placed the ROI properly in the center of a low-contrast object for variations of phantom rotation and tube current, whereas was not properly located in the center of the low-contrast object using the previous method. In addition, from 325 image samples of the 25 CT scanners, the proposed method successfully (100%) located the ROI within the low-contrast objects of all images used. The success rate of the previous method was only 58%.Conclusion. A new method for measuring CNR in the ACR CT phantom has been proposed and implemented. It is more powerful than a previous method based on a threshold approach.

Introduction. The lung CT images of COVID-19 patients can be typically characterized by three different findings- Ground Glass Opacity (GGO), consolidation and pleural effusion. GGOs have been shown to precede consolidations and has different heterogeneous appearance. Conventional severity scoring only uses total area of lung involvement ignoring appearance of the effected regions. This study proposes a baseline to select heterogeneity/radiomic features that can distinguish these three pathological lung findings.Methods. Four approaches were implemented to select features from a pool of 44 features. First one is a manual feature selection method. The rest are automatic feature selection methods based on Genetic Algorithm (GA) coupled with (1) K-Nearest-Neighbor (GA-KNN), (2) binary-decision-tree (GA-BDT) and (3) Artificial-Neural-Network (GA-ANN). For the purpose of validation, an ANN was trained using the selected features and tested on a completely independent data set.Results. Manual selection of nine radiomic features was found to provide the most accurate results with the highest sensitivity, specificity and accuracy (85.7% overall accuracy and 0.90 area under receiver operating characteristic curve) followed by GA-BDT, GA-KNN and GA-ANN (accuracy 78%, 77.5% and 76.8%).Conclusion. Manually selected nine radiomic features can be used in accurate severity scoring allowing the clinician to plan for more effective personalized treatment. They can also be useful for monitoring the progression of COVID-19 and response to therapy for clinical trials.