María Teresa Martínez-Damián, Damaris Leopoldina Ojeda-Barrios, Oscar Cruz-Alvarez
{"title":"杀菌剂、生物控制剂和抗性诱导剂对接种了疫霉的甜椒植株的生物活性化合物和氧化代谢的影响","authors":"María Teresa Martínez-Damián, Damaris Leopoldina Ojeda-Barrios, Oscar Cruz-Alvarez","doi":"10.2478/fhort-2024-0011","DOIUrl":null,"url":null,"abstract":"The rational use of chemical fungicides in chilli pepper (<jats:italic>Capsicum annuum</jats:italic> L.) for controlling <jats:italic>Phytophthora capsici</jats:italic> Leo. should include molecules that promote induced systemic resistance in the plant. The study objective was to assess how the application of fungicides, bio-controllers and resistance inducers affects bioactive compounds production and oxidative metabolism in pepper plants when inoculated with <jats:italic>P. capsici</jats:italic> zoospores. Statistical analyses show significant differences in total flavonoids, total phenols and salicylic acid concentrations in plants treated with <jats:italic>Trichoderma harzianum</jats:italic>, Acibenzolar-S-Methyl (ASM) and phosphonic acid. In addition, the application of ASM and <jats:italic>Bacillus subtilis</jats:italic> showed the highest values of antioxidant capacity. On the other hand, ASM application favoured increases in superoxide dismutase and phenyl ammonium lyase activity with values of 4.42 ± 0.016 (units · min<jats:sup>−1</jats:sup> · g<jats:sup>−1</jats:sup>) and 3.90 ± 0.22 U · g<jats:sup>−1</jats:sup>, respectively. However, hydrogen peroxide production was similar to that of phosphonic acid treatments. Similarly, plants treated with <jats:italic>B. subtilis</jats:italic> and Metalaxyl-M showed increases in peroxidase enzyme activity with values ranging from 2.86 ± 0.05 to 4.52 ± 0.02 (μmol · [mg protein]<jats:sup>−1</jats:sup> · min<jats:sup>−1</jats:sup>). Likewise, phosphonic acid-treated plants exhibited high catalase enzyme activity with values ranging from 2.38 ± 0.032 μmol H<jats:sub>2</jats:sub>O<jats:sub>2</jats:sub> · min<jats:sup>−1</jats:sup> · g<jats:sup>−1</jats:sup> to 4.30 ± 0.024 μmol H<jats:sub>2</jats:sub>O<jats:sub>2</jats:sub> · min<jats:sup>−1</jats:sup> · g<jats:sup>−1</jats:sup>. The complementary use of products with biological and resistance-inducing functions could help to mitigate the environmental impacts that occur due to the use of chemical fungicides for controlling <jats:italic>P. capsici</jats:italic> on chilli pepper.","PeriodicalId":12277,"journal":{"name":"Folia Horticulturae","volume":"68 1","pages":""},"PeriodicalIF":2.2000,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Fungicides, bio-controllers and resistance inducers affect bioactive compounds and oxidative metabolism in bell pepper plants inoculated with Phytophthora capsici\",\"authors\":\"María Teresa Martínez-Damián, Damaris Leopoldina Ojeda-Barrios, Oscar Cruz-Alvarez\",\"doi\":\"10.2478/fhort-2024-0011\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The rational use of chemical fungicides in chilli pepper (<jats:italic>Capsicum annuum</jats:italic> L.) for controlling <jats:italic>Phytophthora capsici</jats:italic> Leo. should include molecules that promote induced systemic resistance in the plant. The study objective was to assess how the application of fungicides, bio-controllers and resistance inducers affects bioactive compounds production and oxidative metabolism in pepper plants when inoculated with <jats:italic>P. capsici</jats:italic> zoospores. Statistical analyses show significant differences in total flavonoids, total phenols and salicylic acid concentrations in plants treated with <jats:italic>Trichoderma harzianum</jats:italic>, Acibenzolar-S-Methyl (ASM) and phosphonic acid. In addition, the application of ASM and <jats:italic>Bacillus subtilis</jats:italic> showed the highest values of antioxidant capacity. On the other hand, ASM application favoured increases in superoxide dismutase and phenyl ammonium lyase activity with values of 4.42 ± 0.016 (units · min<jats:sup>−1</jats:sup> · g<jats:sup>−1</jats:sup>) and 3.90 ± 0.22 U · g<jats:sup>−1</jats:sup>, respectively. However, hydrogen peroxide production was similar to that of phosphonic acid treatments. Similarly, plants treated with <jats:italic>B. subtilis</jats:italic> and Metalaxyl-M showed increases in peroxidase enzyme activity with values ranging from 2.86 ± 0.05 to 4.52 ± 0.02 (μmol · [mg protein]<jats:sup>−1</jats:sup> · min<jats:sup>−1</jats:sup>). Likewise, phosphonic acid-treated plants exhibited high catalase enzyme activity with values ranging from 2.38 ± 0.032 μmol H<jats:sub>2</jats:sub>O<jats:sub>2</jats:sub> · min<jats:sup>−1</jats:sup> · g<jats:sup>−1</jats:sup> to 4.30 ± 0.024 μmol H<jats:sub>2</jats:sub>O<jats:sub>2</jats:sub> · min<jats:sup>−1</jats:sup> · g<jats:sup>−1</jats:sup>. The complementary use of products with biological and resistance-inducing functions could help to mitigate the environmental impacts that occur due to the use of chemical fungicides for controlling <jats:italic>P. capsici</jats:italic> on chilli pepper.\",\"PeriodicalId\":12277,\"journal\":{\"name\":\"Folia Horticulturae\",\"volume\":\"68 1\",\"pages\":\"\"},\"PeriodicalIF\":2.2000,\"publicationDate\":\"2024-09-09\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Folia Horticulturae\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.2478/fhort-2024-0011\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"HORTICULTURE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Folia Horticulturae","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.2478/fhort-2024-0011","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"HORTICULTURE","Score":null,"Total":0}
Fungicides, bio-controllers and resistance inducers affect bioactive compounds and oxidative metabolism in bell pepper plants inoculated with Phytophthora capsici
The rational use of chemical fungicides in chilli pepper (Capsicum annuum L.) for controlling Phytophthora capsici Leo. should include molecules that promote induced systemic resistance in the plant. The study objective was to assess how the application of fungicides, bio-controllers and resistance inducers affects bioactive compounds production and oxidative metabolism in pepper plants when inoculated with P. capsici zoospores. Statistical analyses show significant differences in total flavonoids, total phenols and salicylic acid concentrations in plants treated with Trichoderma harzianum, Acibenzolar-S-Methyl (ASM) and phosphonic acid. In addition, the application of ASM and Bacillus subtilis showed the highest values of antioxidant capacity. On the other hand, ASM application favoured increases in superoxide dismutase and phenyl ammonium lyase activity with values of 4.42 ± 0.016 (units · min−1 · g−1) and 3.90 ± 0.22 U · g−1, respectively. However, hydrogen peroxide production was similar to that of phosphonic acid treatments. Similarly, plants treated with B. subtilis and Metalaxyl-M showed increases in peroxidase enzyme activity with values ranging from 2.86 ± 0.05 to 4.52 ± 0.02 (μmol · [mg protein]−1 · min−1). Likewise, phosphonic acid-treated plants exhibited high catalase enzyme activity with values ranging from 2.38 ± 0.032 μmol H2O2 · min−1 · g−1 to 4.30 ± 0.024 μmol H2O2 · min−1 · g−1. The complementary use of products with biological and resistance-inducing functions could help to mitigate the environmental impacts that occur due to the use of chemical fungicides for controlling P. capsici on chilli pepper.
期刊介绍:
Folia Horticulturae is an international, scientific journal published in English. It covers a broad research spectrum of aspects related to horticultural science that are of interest to a wide scientific community and have an impact on progress in both basic and applied research carried out with the use of horticultural crops and their products. The journal’s aim is to disseminate recent findings and serve as a forum for presenting views as well as for discussing important problems and prospects of modern horticulture, particularly in relation to sustainable production of high yield and quality of horticultural products, including their impact on human health.