Yanling Xie, Shuai Wang, Yao Liu, Jie Deng, Xiaoling Su, Zhichu Huang, Huoqing Zheng
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引用次数: 0
摘要
幼虫死亡是患病蜜蜂蜂群的主要症状,通常归因于囊尾蚴病毒(SBV)和羽褐曲霉(Melissococcus plutonius)。然而,其他常见蜜蜂病毒的影响经常被忽视,而且它们对蜜蜂的致病性仍然知之甚少。为了研究蜜蜂雏蜂发病率不断上升的原因,我们进行了一次流行病学调查,从中国 9 个省的 19 个地点收集了 70 份样本。此外,我们还通过人工接种实验研究了以色列急性麻痹病毒(IAPV)在鸡雏中的致病性。结果表明,除 SBV 和 M. plutonius 外,IAPV 在患病雏鸟中的感染率和病毒载量都很高。人工接种高浓度 IAPV 的雏鸡死亡率显著增加,并表现出与自然感染雏鸡群相似的临床症状。此外,在 A. cerana 雏鸟中观察到了对 IAPV 的有限抵抗力,一些个体能够限制病毒的增殖。我们的研究强调了 IAPV 对 A. cerana 雏鸟的致病性,表明 IAPV 与 SBV 和 M. plutonius 一样构成重大威胁。我们强调,应将 IAPV 视为导致陶瓷蜂育雏疾病的新病原体,并在养蜂实践中进行相应的管理。
Israeli Acute Paralysis Virus Is an Emerging Pathogen Contributing to Brood Disease of Apis cerana
Larval mortality is the primary symptom of diseased Apis cerana colonies, often attributed to sacbrood virus (SBV) and Melissococcus plutonius. However, the impact of other common honeybee viruses is frequently overlooked, and their pathogenicity to A. cerana remains poorly understood. To investigate the causes of the increasing disease incidence in A. cerana brood, we conducted an epidemiological survey, collecting 70 samples from 19 sites across nine provinces in China. Furthermore, we examined the pathogenicity of Israeli acute paralysis virus (IAPV) in A. cerana brood through artificial inoculation experiments. Our results demonstrate that, besides SBV and M. plutonius, the infection rate and viral load of IAPV in diseased brood are significantly high. Brood artificially inoculated with high concentrations of IAPV exhibited a significant increase in mortality and displayed clinical symptoms similar to those observed in naturally infected colonies. Moreover, a limited resistance to IAPV was observed in A. cerana brood, with some individuals able to restrict viral proliferation. Our study highlights the previously unrecognized pathogenicity of IAPV to A. cerana brood, demonstrating that IAPV poses a significant threat similar to SBV and M. plutonius. We emphasize that IAPV should be recognized as an emerging pathogen causing brood disease in A. cerana and managed accordingly in beekeeping practices.