Yanhua Zhang, Chan Hu, Ronghui Liu, Shujun He, Jie Yang, Wen Yao, Yi Li, Xinrong Guo
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引用次数: 0
摘要
基于高通量和无标记蛋白质纳米孔的传感器被广泛应用于 DNA 测序、单个蛋白质分析、分子传感和单通道记录的化学催化。这些技术解决了现有技术在便携性和速度方面的局限性,在识别与公众健康有关的各种有害物质方面显示出巨大的潜力。在本综述中,我们将概述纳米孔传感的基本原理,重点介绍旨在提高蛋白质纳米孔检测灵敏度和识别准确性的化学修饰和基因工程策略。工程化的蛋白质纳米孔可以实现直接传感,而引入适配体和底物则可以实现间接传感,将分析物的物理结构和化学性质转化为可读信号。这些科学发现和工程努力为检测和监测痕量有害物质提供了新的前景。
Protein nanopore-based sensors for public health analyte detection
High-throughput and label-free protein nanopore-based sensors are extensively used in DNA sequencing, single-protein analysis, molecular sensing and chemical catalysis with single channel recording. These technologies show great potential for identifying various harmful substances linked to public health by addressing the limitations of current portability and the speed of existing techniques. In this review, we provide an overview of the fundamental principles of nanopore sensing, with a focus on chemical modification and genetic engineering strategies aimed at enhancing the detection sensitivity and identification accuracy of protein nanopores. The engineered protein nanopores enable direct sensing, while the introduction of aptamers and substrates enables indirect sensing, translating the physical structure and chemical properties of analytes into readable signals. These scientific discoveries and engineering efforts have provided new prospects for detecting and monitoring trace hazardous substances.
期刊介绍:
Journal of Materials Chemistry A, B & C cover high quality studies across all fields of materials chemistry. The journals focus on those theoretical or experimental studies that report new understanding, applications, properties and synthesis of materials. Journal of Materials Chemistry A, B & C are separated by the intended application of the material studied. Broadly, applications in energy and sustainability are of interest to Journal of Materials Chemistry A, applications in biology and medicine are of interest to Journal of Materials Chemistry B, and applications in optical, magnetic and electronic devices are of interest to Journal of Materials Chemistry C.Journal of Materials Chemistry B is a Transformative Journal and Plan S compliant. Example topic areas within the scope of Journal of Materials Chemistry B are listed below. This list is neither exhaustive nor exclusive:
Antifouling coatings
Biocompatible materials
Bioelectronics
Bioimaging
Biomimetics
Biomineralisation
Bionics
Biosensors
Diagnostics
Drug delivery
Gene delivery
Immunobiology
Nanomedicine
Regenerative medicine & Tissue engineering
Scaffolds
Soft robotics
Stem cells
Therapeutic devices