{"title":"从山茶提取物中提取紫外线激活的绿色合成氧化锌纳米粒子:一种有效的抗菌策略","authors":"Namrata Roy, Krishnan Kannabiran","doi":"10.1007/s11696-024-03641-w","DOIUrl":null,"url":null,"abstract":"<div><p>This study provides valuable insights into the structural and chemical characteristics of zinc oxide nanoparticles (ZnO NPs), enhancing their antimicrobial efficacy against Gram-positive bacteria and fungi. Structural and optical analyses of ZnO NPs synthesized via a green route revealed a floral morphology at 450 °C with petal sizes averaging ~ 20.56 nm. Size and strain characteristics were extensively investigated using mathematical techniques such as the Scherrer and Williamson–Hall methods. The influence of key parameters, including nanoparticle concentration and UV exposure, on the antimicrobial efficacy of UV-irradiated ZnO nanoparticles was examined. Biochemical assays suggest that the synthesized ZnO nanoparticles hold potential as novel antimicrobial agents for effectively controlling microbial infections. Notably, oxidative stress markers showed a significant increase in protein carbonyl formation in <i>Staphylococcus aureus</i> (31,237 nmol/mg) and <i>Candida albicans</i> (29,109 nmol/mg). Additionally, the time-dependent antimicrobial effect revealed reduction in microbial growth over specified periods, indicating prolonged antimicrobial activity. The molecular-level study demonstrates that the antimicrobial activity of ZnO NPs, particularly upon UV activation, is mediated through the production of ROS, leading to oxidative stress, protein damage, enzymatic activity disruption, and membrane integrity compromise, ultimately resulting in microbial cell death.</p></div>","PeriodicalId":513,"journal":{"name":"Chemical Papers","volume":"78 14","pages":"7877 - 7894"},"PeriodicalIF":2.2000,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"UV-activated green-synthesized ZnO NPs from Camellia sinensis extract: a potent antimicrobial strategy\",\"authors\":\"Namrata Roy, Krishnan Kannabiran\",\"doi\":\"10.1007/s11696-024-03641-w\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>This study provides valuable insights into the structural and chemical characteristics of zinc oxide nanoparticles (ZnO NPs), enhancing their antimicrobial efficacy against Gram-positive bacteria and fungi. Structural and optical analyses of ZnO NPs synthesized via a green route revealed a floral morphology at 450 °C with petal sizes averaging ~ 20.56 nm. Size and strain characteristics were extensively investigated using mathematical techniques such as the Scherrer and Williamson–Hall methods. The influence of key parameters, including nanoparticle concentration and UV exposure, on the antimicrobial efficacy of UV-irradiated ZnO nanoparticles was examined. Biochemical assays suggest that the synthesized ZnO nanoparticles hold potential as novel antimicrobial agents for effectively controlling microbial infections. Notably, oxidative stress markers showed a significant increase in protein carbonyl formation in <i>Staphylococcus aureus</i> (31,237 nmol/mg) and <i>Candida albicans</i> (29,109 nmol/mg). Additionally, the time-dependent antimicrobial effect revealed reduction in microbial growth over specified periods, indicating prolonged antimicrobial activity. The molecular-level study demonstrates that the antimicrobial activity of ZnO NPs, particularly upon UV activation, is mediated through the production of ROS, leading to oxidative stress, protein damage, enzymatic activity disruption, and membrane integrity compromise, ultimately resulting in microbial cell death.</p></div>\",\"PeriodicalId\":513,\"journal\":{\"name\":\"Chemical Papers\",\"volume\":\"78 14\",\"pages\":\"7877 - 7894\"},\"PeriodicalIF\":2.2000,\"publicationDate\":\"2024-08-13\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Chemical Papers\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s11696-024-03641-w\",\"RegionNum\":4,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"Engineering\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chemical Papers","FirstCategoryId":"92","ListUrlMain":"https://link.springer.com/article/10.1007/s11696-024-03641-w","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"Engineering","Score":null,"Total":0}
UV-activated green-synthesized ZnO NPs from Camellia sinensis extract: a potent antimicrobial strategy
This study provides valuable insights into the structural and chemical characteristics of zinc oxide nanoparticles (ZnO NPs), enhancing their antimicrobial efficacy against Gram-positive bacteria and fungi. Structural and optical analyses of ZnO NPs synthesized via a green route revealed a floral morphology at 450 °C with petal sizes averaging ~ 20.56 nm. Size and strain characteristics were extensively investigated using mathematical techniques such as the Scherrer and Williamson–Hall methods. The influence of key parameters, including nanoparticle concentration and UV exposure, on the antimicrobial efficacy of UV-irradiated ZnO nanoparticles was examined. Biochemical assays suggest that the synthesized ZnO nanoparticles hold potential as novel antimicrobial agents for effectively controlling microbial infections. Notably, oxidative stress markers showed a significant increase in protein carbonyl formation in Staphylococcus aureus (31,237 nmol/mg) and Candida albicans (29,109 nmol/mg). Additionally, the time-dependent antimicrobial effect revealed reduction in microbial growth over specified periods, indicating prolonged antimicrobial activity. The molecular-level study demonstrates that the antimicrobial activity of ZnO NPs, particularly upon UV activation, is mediated through the production of ROS, leading to oxidative stress, protein damage, enzymatic activity disruption, and membrane integrity compromise, ultimately resulting in microbial cell death.
Chemical PapersChemical Engineering-General Chemical Engineering
CiteScore
3.30
自引率
4.50%
发文量
590
期刊介绍:
Chemical Papers is a peer-reviewed, international journal devoted to basic and applied chemical research. It has a broad scope covering the chemical sciences, but favors interdisciplinary research and studies that bring chemistry together with other disciplines.