从山茶提取物中提取紫外线激活的绿色合成氧化锌纳米粒子:一种有效的抗菌策略

IF 2.2 4区 化学 Q2 Engineering Chemical Papers Pub Date : 2024-08-13 DOI:10.1007/s11696-024-03641-w
Namrata Roy, Krishnan Kannabiran
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摘要

这项研究为了解氧化锌纳米粒子(ZnO NPs)的结构和化学特性提供了宝贵的见解,从而提高了它们对革兰氏阳性细菌和真菌的抗菌功效。通过绿色路线合成的氧化锌纳米粒子的结构和光学分析表明,在 450 ℃ 下呈现花朵形态,花瓣大小平均约为 20.56 nm。利用舍勒法和威廉森-霍尔法等数学技术对尺寸和应变特性进行了广泛研究。研究了纳米颗粒浓度和紫外线照射等关键参数对紫外线照射氧化锌纳米颗粒抗菌效果的影响。生化分析表明,合成的氧化锌纳米粒子具有作为新型抗菌剂的潜力,可有效控制微生物感染。值得注意的是,氧化应激标记显示,金黄色葡萄球菌(31237 nmol/mg)和白色念珠菌(29109 nmol/mg)的蛋白质羰基形成显著增加。此外,随时间变化的抗菌效果显示,在特定时间段内微生物的生长速度有所下降,这表明抗菌活性持续时间较长。这项分子水平的研究表明,氧化锌纳米粒子的抗菌活性,尤其是在紫外线激活时,是通过产生 ROS 来介导的,从而导致氧化应激、蛋白质损伤、酶活性破坏和膜完整性受损,最终导致微生物细胞死亡。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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UV-activated green-synthesized ZnO NPs from Camellia sinensis extract: a potent antimicrobial strategy

This study provides valuable insights into the structural and chemical characteristics of zinc oxide nanoparticles (ZnO NPs), enhancing their antimicrobial efficacy against Gram-positive bacteria and fungi. Structural and optical analyses of ZnO NPs synthesized via a green route revealed a floral morphology at 450 °C with petal sizes averaging ~ 20.56 nm. Size and strain characteristics were extensively investigated using mathematical techniques such as the Scherrer and Williamson–Hall methods. The influence of key parameters, including nanoparticle concentration and UV exposure, on the antimicrobial efficacy of UV-irradiated ZnO nanoparticles was examined. Biochemical assays suggest that the synthesized ZnO nanoparticles hold potential as novel antimicrobial agents for effectively controlling microbial infections. Notably, oxidative stress markers showed a significant increase in protein carbonyl formation in Staphylococcus aureus (31,237 nmol/mg) and Candida albicans (29,109 nmol/mg). Additionally, the time-dependent antimicrobial effect revealed reduction in microbial growth over specified periods, indicating prolonged antimicrobial activity. The molecular-level study demonstrates that the antimicrobial activity of ZnO NPs, particularly upon UV activation, is mediated through the production of ROS, leading to oxidative stress, protein damage, enzymatic activity disruption, and membrane integrity compromise, ultimately resulting in microbial cell death.

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来源期刊
Chemical Papers
Chemical Papers Chemical Engineering-General Chemical Engineering
CiteScore
3.30
自引率
4.50%
发文量
590
期刊介绍: Chemical Papers is a peer-reviewed, international journal devoted to basic and applied chemical research. It has a broad scope covering the chemical sciences, but favors interdisciplinary research and studies that bring chemistry together with other disciplines.
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