Jun-Yi Wang , Hsuan-Yi Hsu , Sian-Tai Liu , Chia-Hao Lin
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引用次数: 0
摘要
氨是淡水环境中的一种主要污染物。以往的研究表明,接触氨气会对淡水鱼的生理产生不利影响。然而,氨对淡水鱼幼体骨矿化的影响仍不清楚。本研究以斑马鱼幼体为模型,研究不同氨氮水平(0、2.5、5 和 10 mM NH4Cl)对鱼类存活率、体长和骨矿化的影响。暴露在不同浓度 NH4Cl 中 8 天的斑马鱼胚胎的存活率未受影响。相反,斑马鱼幼体在受精后 8 天(dpf)的体长和骨矿化度在暴露于 5 毫摩尔和 10 毫摩尔 NH4Cl 时显著降低。进一步研究发现,暴露于氨气会降低成骨细胞相关基因的 mRNA 表达,增加破骨细胞相关基因的 mRNA 表达。此外,暴露于 5 mM 和 10 mM NH4Cl 会诱导活性氧(ROS)的产生。暴露于 10 mM 而非 5 mM NH4Cl 会降低斑马鱼 8 dpf 幼体中的钙和磷含量。总之,暴露于氨诱导骨吸收,同时降低全身的钙和磷含量以及骨形成,导致鱼类幼体骨矿化受损。
Ammonia exposure impairs bone mineralization in zebrafish (Danio rerio) larvae
Ammonia is a major pollutant of freshwater environments. Previous studies have indicated that ammonia exposure adversely affects the physiology of freshwater fish. However, its effect on bone mineralization in freshwater fish larvae remains unclear. In this study, zebrafish larvae were used as a model to investigate the effects of different ammonia levels (0, 2.5, 5, and 10 mM NH4Cl) on the survival rate, body length, and bone mineralization of fish. The survival rate of zebrafish embryos exposed to different NH4Cl concentrations for 8 days was not affected. In contrast, the body length and bone mineralization of zebrafish larvae at 8 days post fertilization (dpf) were significantly reduced at 5 and 10 mM NH4Cl exposure. Further investigations revealed that ammonia exposure decreased the mRNA expression of osteoblast-related genes and increased that of osteoclast-related genes. Additionally, exposure to 5 mM and 10 mM NH4Cl induced the production of reactive oxygen species (ROS). 10 mM—but not 5 mM—NH4Cl exposure reduced the calcium and phosphorus content in 8 dpf zebrafish larvae. In conclusion, ammonia exposure induces bone resorption, while decreasing the calcium and phosphorus content of the whole body and bone formation, resulting in impaired bone mineralization in fish larvae.
期刊介绍:
Part C: Toxicology and Pharmacology. This journal is concerned with chemical and drug action at different levels of organization, biotransformation of xenobiotics, mechanisms of toxicity, including reactive oxygen species and carcinogenesis, endocrine disruptors, natural products chemistry, and signal transduction with a molecular approach to these fields.