{"title":"基于根瘤微生物组、根转录组和代谢组的甘蓝型油菜在二氧化碳浓度升高条件下对土壤镉的响应","authors":"","doi":"10.1016/j.plaphy.2024.109127","DOIUrl":null,"url":null,"abstract":"<div><p>Rising atmospheric carbon dioxide (CO<sub>2</sub>) and soil heavy metal pollution affect crop safety and production. Exposure to elevated CO<sub>2</sub> (ECO<sub>2</sub>) increases cadmium (Cd) uptake in some crops like wheat and rice, however, it remains unclear how ECO<sub>2</sub> affects Cd uptake by <em>Brassica napus</em>. Here, we investigated the responses of <em>B. napus</em> seedlings exposed to ECO<sub>2</sub> and Cd through analyses of physiology, transcriptome, metabolome, and rhizosphere microbes. Compared with Cd-stress alone (Cd50_ACO<sub>2</sub>), ECO<sub>2</sub> boosted the uptake of Cd by <em>B. napus</em> roots by 38.78% under coupled stresses (Cd50_ECO<sub>2</sub>). The biomass and leaf chlorophyll <em>a</em> content increased by 38.49% and 79.66% respectively in Cd50_ECO<sub>2</sub> relative to Cd50_ACO<sub>2</sub>. Activities of superoxide dismutase (SOD) and peroxidase (POD) enhanced by 8.42% and 185.01%, respectively, while glutathione (GSH) and ascorbic acid (AsA) contents increased by 16.44% and 52.48%, and abundances of rhizosphere microbes changed significantly under coupled stresses (Cd50_ECO<sub>2</sub>) relative to Cd-stress alone (Cd50_ACO<sub>2</sub>). Also, the upregulation of glutathione, glutathione transferase genes, and heavy metal ATPase expression promoted the detoxification effect of rapeseed on Cd. Changes in the expression of transcription factors like MAPK, WRKY, BAK1 and PR1, as well as changes in metabolic pathways like β-alanine, may be involved in the regulatory mechanism of stress response. These findings provide new insights for studying the regulatory mechanism of rapeseed under ECO<sub>2</sub> on soil Cd stress, and also provide a basis for further research on Cd tolerant rapeseed varieties in the future climate context.</p></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":null,"pages":null},"PeriodicalIF":6.1000,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Responses of Brassica napus to soil cadmium under elevated CO2 concentration based on rhizosphere microbiome, root transcriptome and metabolome\",\"authors\":\"\",\"doi\":\"10.1016/j.plaphy.2024.109127\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Rising atmospheric carbon dioxide (CO<sub>2</sub>) and soil heavy metal pollution affect crop safety and production. Exposure to elevated CO<sub>2</sub> (ECO<sub>2</sub>) increases cadmium (Cd) uptake in some crops like wheat and rice, however, it remains unclear how ECO<sub>2</sub> affects Cd uptake by <em>Brassica napus</em>. Here, we investigated the responses of <em>B. napus</em> seedlings exposed to ECO<sub>2</sub> and Cd through analyses of physiology, transcriptome, metabolome, and rhizosphere microbes. Compared with Cd-stress alone (Cd50_ACO<sub>2</sub>), ECO<sub>2</sub> boosted the uptake of Cd by <em>B. napus</em> roots by 38.78% under coupled stresses (Cd50_ECO<sub>2</sub>). The biomass and leaf chlorophyll <em>a</em> content increased by 38.49% and 79.66% respectively in Cd50_ECO<sub>2</sub> relative to Cd50_ACO<sub>2</sub>. Activities of superoxide dismutase (SOD) and peroxidase (POD) enhanced by 8.42% and 185.01%, respectively, while glutathione (GSH) and ascorbic acid (AsA) contents increased by 16.44% and 52.48%, and abundances of rhizosphere microbes changed significantly under coupled stresses (Cd50_ECO<sub>2</sub>) relative to Cd-stress alone (Cd50_ACO<sub>2</sub>). Also, the upregulation of glutathione, glutathione transferase genes, and heavy metal ATPase expression promoted the detoxification effect of rapeseed on Cd. Changes in the expression of transcription factors like MAPK, WRKY, BAK1 and PR1, as well as changes in metabolic pathways like β-alanine, may be involved in the regulatory mechanism of stress response. These findings provide new insights for studying the regulatory mechanism of rapeseed under ECO<sub>2</sub> on soil Cd stress, and also provide a basis for further research on Cd tolerant rapeseed varieties in the future climate context.</p></div>\",\"PeriodicalId\":20234,\"journal\":{\"name\":\"Plant Physiology and Biochemistry\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":6.1000,\"publicationDate\":\"2024-09-13\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Plant Physiology and Biochemistry\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0981942824007952\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Physiology and Biochemistry","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0981942824007952","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0
摘要
大气中二氧化碳(CO2)的升高和土壤重金属污染会影响作物的安全和产量。暴露于高浓度二氧化碳(ECO2)会增加一些作物(如小麦和水稻)对镉(Cd)的吸收,然而,ECO2如何影响甘蓝型油菜对镉的吸收仍不清楚。在这里,我们通过对生理、转录组、代谢组和根瘤微生物的分析,研究了暴露在 ECO2 和镉胁迫下的油菜幼苗的反应。与单独的镉胁迫(Cd50_ACO2)相比,在耦合胁迫(Cd50_ECO2)下,ECO2使油菜根系对镉的吸收提高了38.78%。与 Cd50_ACO2 相比,Cd50_ECO2 的生物量和叶片叶绿素 a 含量分别增加了 38.49% 和 79.66%。超氧化物歧化酶(SOD)和过氧化物酶(POD)的活性分别提高了 8.42% 和 185.01%,谷胱甘肽(GSH)和抗坏血酸(AsA)的含量分别提高了 16.44% 和 52.48%,根瘤微生物的丰度在耦合胁迫(Cd50_ECO2)下相对于单独的 Cd 胁迫(Cd50_ACO2)发生了显著变化。此外,谷胱甘肽、谷胱甘肽转移酶基因和重金属 ATP 酶表达的上调也促进了油菜籽对镉的解毒作用。MAPK、WRKY、BAK1和PR1等转录因子的表达变化以及β-丙氨酸等代谢途径的变化可能参与了胁迫响应的调控机制。这些发现为研究油菜在ECO2土壤镉胁迫下的调控机制提供了新的见解,也为在未来气候背景下进一步研究耐镉油菜品种提供了依据。
Responses of Brassica napus to soil cadmium under elevated CO2 concentration based on rhizosphere microbiome, root transcriptome and metabolome
Rising atmospheric carbon dioxide (CO2) and soil heavy metal pollution affect crop safety and production. Exposure to elevated CO2 (ECO2) increases cadmium (Cd) uptake in some crops like wheat and rice, however, it remains unclear how ECO2 affects Cd uptake by Brassica napus. Here, we investigated the responses of B. napus seedlings exposed to ECO2 and Cd through analyses of physiology, transcriptome, metabolome, and rhizosphere microbes. Compared with Cd-stress alone (Cd50_ACO2), ECO2 boosted the uptake of Cd by B. napus roots by 38.78% under coupled stresses (Cd50_ECO2). The biomass and leaf chlorophyll a content increased by 38.49% and 79.66% respectively in Cd50_ECO2 relative to Cd50_ACO2. Activities of superoxide dismutase (SOD) and peroxidase (POD) enhanced by 8.42% and 185.01%, respectively, while glutathione (GSH) and ascorbic acid (AsA) contents increased by 16.44% and 52.48%, and abundances of rhizosphere microbes changed significantly under coupled stresses (Cd50_ECO2) relative to Cd-stress alone (Cd50_ACO2). Also, the upregulation of glutathione, glutathione transferase genes, and heavy metal ATPase expression promoted the detoxification effect of rapeseed on Cd. Changes in the expression of transcription factors like MAPK, WRKY, BAK1 and PR1, as well as changes in metabolic pathways like β-alanine, may be involved in the regulatory mechanism of stress response. These findings provide new insights for studying the regulatory mechanism of rapeseed under ECO2 on soil Cd stress, and also provide a basis for further research on Cd tolerant rapeseed varieties in the future climate context.
期刊介绍:
Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement.
Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB.
Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.