Cong Li, Bingcheng Yi, Quanchen Xu, Jinlong Ma, Luhan Yuan, Yining Liu, Wei Liu, Ziyi Zhou, Xuchao Ning, Jierui Zhang, Fan Yang, Sisi Wang, Qiang Shi, Qihui Zhou, Zhiguo Wang
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引用次数: 0
摘要
病理疤痕(PS)与炎症的过度反应和肌成纤维细胞的过度激活有关。本文开发了一种新型微针(MN)贴片,该贴片基于脂肪源性干细胞浓缩条件培养基(ADSCC-CM)交联角蛋白水凝胶,可负载曲安奈德(TA),从而实现ADSCC-CM和TA的双重给药,以同时减轻炎症和引导肌成纤维细胞行为。研究结果不仅证实了 ADSCC-CM 推动角蛋白水凝胶形成的能力,还验证了水凝胶开发的 MNs(TA@AC-MN)的双重药物释放能力。利用人体增生性疤痕成纤维细胞(HSF),ADSCC-CM和TA的组合在减轻炎症微环境对HSF的有害影响方面显示出明显的协同效应,包括抑制活性氧的产生和减轻炎症因子的表达。与临床使用的 TA 相比,TA@AC-MN 可促进疤痕的仿生修复(即优化胶原蛋白的比例和提高组织的抗拉强度)。这项研究表明,TA@AC-MN 有能力为 PS 提供一种自我管理和微创的治疗策略。
ADSCC-CM-Induced Keratin Hydrogel-Based Bioactive Microneedle Patch Containing Triamcinolone Acetonide for the Treatment of Pathological Scar
Pathological scars (PS) are involved in the excessive response of inflammation and overactivation of myofibroblasts. Herein, a novel microneedle (MN) patch based on the adipose-derived stem cell concentrated conditioned medium (ADSCC-CM) cross-linked keratin hydrogel is developed to load triamcinolone acetonide (TA), thereby achieving the dual-drug delivery of ADSCC-CM and TA to simultaneously reduce the inflammation and guide myofibroblast behaviors. Results not only confirm the ability of ADSCC-CM to drive the formation of keratin-based hydrogel, but also verify the dual-drug release capacities of the hydrogel-developed MNs (TA@AC-MN). Using human hyperplastic scar fibroblast (HSF), the combination of ADSCC-CM and TA demonstrates a pronounced synergistic effect in mitigating the detrimental effects of the inflammatory microenvironment on HSFs, including suppressing the production of reactive oxygen species and attenuating the expression of inflammatory factors. Compared with the clinically used TA, TA@AC-MN promotes scar biomimetic repair (i.e., optimizing the proportion of collagen and increasing the tissue tensile strength). This study demonstrates that TA@AC-MN has the capacity to provide a self-managing and minimally invasive therapeutic strategy for PS.
期刊介绍:
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