Aegilops umbellulata 的基因组为小麦改良提供了关于 U 基因组独特结构变异和遗传多样性的新见解

IF 10.1 1区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Plant Biotechnology Journal Pub Date : 2024-09-18 DOI:10.1111/pbi.14470
Jatinder Singh, Santosh Gudi, Peter J. Maughan, Zhaohui Liu, James Kolmer, Meinan Wang, Xianming Chen, Matthew N. Rouse, Pauline Lasserre-Zuber, Héléne Rimbert, Sunish Sehgal, Jason D. Fiedler, Frédéric Choulet, Maricelis Acevedo, Rajeev Gupta, Upinder Gill
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引用次数: 0

摘要

Aegilops umbellulata是改良小麦的新型生物和非生物胁迫耐受性的重要资源库。然而,该物种的染色体重排和进化轨迹仍有待阐明。在这里,我们通过对 PI 554389 进行近端粒到端粒的高质量基因组组装,并对另外 20 个代表不同地理和表型变异的 Ae. umbellulata 基因组进行了重新测序,从而对 Ae. umbellulata 基因组进行了全面研究。我们的分析揭示了复杂的染色体重排,其中最突出的是 4U 和 6U 染色体,勾勒出了伞形花序姬蜂与小麦及其近缘种的不同进化轨迹。此外,我们的数据纠正了过去对 4U 和 6U 染色体的错误命名,并强调了导致当今 U 基因组的多个主要进化事件。对不同的伞形花序(Ae. umbellulata)样本进行重测序发现,该物种内部具有高度遗传多样性,可划分为三个不同的进化亚群,并在抗五种主要小麦病害的多个种族/病型方面具有广泛的表型变异性。病害评估表明,重测序品系中存在多个新型抗病基因,可供今后研究使用。重新测序还为 Lr9 确定了六个新的单倍型,Lr9 是第一个从伞形花序 Ae. 克隆的抗性基因。本研究中展示的大量基因组和表型资源将加快未来对伞形花序姬蜂的遗传探索,促进旨在提高小麦抗逆性和生产力的工作。
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Genomes of Aegilops umbellulata provide new insights into unique structural variations and genetic diversity in the U-genome for wheat improvement
Aegilops umbellulata serve as an important reservoir for novel biotic and abiotic stress tolerance for wheat improvement. However, chromosomal rearrangements and evolutionary trajectory of this species remain to be elucidated. Here, we present a comprehensive investigation into Ae. umbellulata genome by generating a high-quality near telomere-to-telomere genome assembly of PI 554389 and resequencing 20 additional Ae. umbellulata genomes representing diverse geographical and phenotypic variations. Our analysis unveils complex chromosomal rearrangements, most prominently in 4U and 6U chromosomes, delineating a distinct evolutionary trajectory of Ae. umbellulata from wheat and its relatives. Furthermore, our data rectified the erroneous naming of chromosomes 4U and 6U in the past and highlighted multiple major evolutionary events that led to the present-day U-genome. Resequencing of diverse Ae. umbellulata accessions revealed high genetic diversity within the species, partitioning into three distinct evolutionary sub-populations and supported by extensive phenotypic variability in resistance against several races/pathotypes of five major wheat diseases. Disease evaluations indicated the presence of several novel resistance genes in the resequenced lines for future studies. Resequencing also resulted in the identification of six new haplotypes for Lr9, the first resistance gene cloned from Ae. umbellulata. The extensive genomic and phenotypic resources presented in this study will expedite the future genetic exploration of Ae. umbellulata, facilitating efforts aimed at enhancing resiliency and productivity in wheat.
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来源期刊
Plant Biotechnology Journal
Plant Biotechnology Journal 生物-生物工程与应用微生物
CiteScore
20.50
自引率
2.90%
发文量
201
审稿时长
1 months
期刊介绍: Plant Biotechnology Journal aspires to publish original research and insightful reviews of high impact, authored by prominent researchers in applied plant science. The journal places a special emphasis on molecular plant sciences and their practical applications through plant biotechnology. Our goal is to establish a platform for showcasing significant advances in the field, encompassing curiosity-driven studies with potential applications, strategic research in plant biotechnology, scientific analysis of crucial issues for the beneficial utilization of plant sciences, and assessments of the performance of plant biotechnology products in practical applications.
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