Jatinder Singh, Santosh Gudi, Peter J. Maughan, Zhaohui Liu, James Kolmer, Meinan Wang, Xianming Chen, Matthew N. Rouse, Pauline Lasserre-Zuber, Héléne Rimbert, Sunish Sehgal, Jason D. Fiedler, Frédéric Choulet, Maricelis Acevedo, Rajeev Gupta, Upinder Gill
{"title":"Aegilops umbellulata 的基因组为小麦改良提供了关于 U 基因组独特结构变异和遗传多样性的新见解","authors":"Jatinder Singh, Santosh Gudi, Peter J. Maughan, Zhaohui Liu, James Kolmer, Meinan Wang, Xianming Chen, Matthew N. Rouse, Pauline Lasserre-Zuber, Héléne Rimbert, Sunish Sehgal, Jason D. Fiedler, Frédéric Choulet, Maricelis Acevedo, Rajeev Gupta, Upinder Gill","doi":"10.1111/pbi.14470","DOIUrl":null,"url":null,"abstract":"<i>Aegilops umbellulata</i> serve as an important reservoir for novel biotic and abiotic stress tolerance for wheat improvement. However, chromosomal rearrangements and evolutionary trajectory of this species remain to be elucidated. Here, we present a comprehensive investigation into <i>Ae. umbellulata</i> genome by generating a high-quality near telomere-to-telomere genome assembly of PI 554389 and resequencing 20 additional <i>Ae. umbellulata</i> genomes representing diverse geographical and phenotypic variations. Our analysis unveils complex chromosomal rearrangements, most prominently in 4U and 6U chromosomes, delineating a distinct evolutionary trajectory of <i>Ae. umbellulata</i> from wheat and its relatives. Furthermore, our data rectified the erroneous naming of chromosomes 4U and 6U in the past and highlighted multiple major evolutionary events that led to the present-day U-genome. Resequencing of diverse <i>Ae. umbellulata</i> accessions revealed high genetic diversity within the species, partitioning into three distinct evolutionary sub-populations and supported by extensive phenotypic variability in resistance against several races/pathotypes of five major wheat diseases. Disease evaluations indicated the presence of several novel resistance genes in the resequenced lines for future studies. Resequencing also resulted in the identification of six new haplotypes for <i>Lr9</i>, the first resistance gene cloned from <i>Ae. umbellulata.</i> The extensive genomic and phenotypic resources presented in this study will expedite the future genetic exploration of <i>Ae. umbellulata</i>, facilitating efforts aimed at enhancing resiliency and productivity in wheat.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"30 1","pages":""},"PeriodicalIF":10.1000,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Genomes of Aegilops umbellulata provide new insights into unique structural variations and genetic diversity in the U-genome for wheat improvement\",\"authors\":\"Jatinder Singh, Santosh Gudi, Peter J. Maughan, Zhaohui Liu, James Kolmer, Meinan Wang, Xianming Chen, Matthew N. 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Furthermore, our data rectified the erroneous naming of chromosomes 4U and 6U in the past and highlighted multiple major evolutionary events that led to the present-day U-genome. Resequencing of diverse <i>Ae. umbellulata</i> accessions revealed high genetic diversity within the species, partitioning into three distinct evolutionary sub-populations and supported by extensive phenotypic variability in resistance against several races/pathotypes of five major wheat diseases. Disease evaluations indicated the presence of several novel resistance genes in the resequenced lines for future studies. 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Genomes of Aegilops umbellulata provide new insights into unique structural variations and genetic diversity in the U-genome for wheat improvement
Aegilops umbellulata serve as an important reservoir for novel biotic and abiotic stress tolerance for wheat improvement. However, chromosomal rearrangements and evolutionary trajectory of this species remain to be elucidated. Here, we present a comprehensive investigation into Ae. umbellulata genome by generating a high-quality near telomere-to-telomere genome assembly of PI 554389 and resequencing 20 additional Ae. umbellulata genomes representing diverse geographical and phenotypic variations. Our analysis unveils complex chromosomal rearrangements, most prominently in 4U and 6U chromosomes, delineating a distinct evolutionary trajectory of Ae. umbellulata from wheat and its relatives. Furthermore, our data rectified the erroneous naming of chromosomes 4U and 6U in the past and highlighted multiple major evolutionary events that led to the present-day U-genome. Resequencing of diverse Ae. umbellulata accessions revealed high genetic diversity within the species, partitioning into three distinct evolutionary sub-populations and supported by extensive phenotypic variability in resistance against several races/pathotypes of five major wheat diseases. Disease evaluations indicated the presence of several novel resistance genes in the resequenced lines for future studies. Resequencing also resulted in the identification of six new haplotypes for Lr9, the first resistance gene cloned from Ae. umbellulata. The extensive genomic and phenotypic resources presented in this study will expedite the future genetic exploration of Ae. umbellulata, facilitating efforts aimed at enhancing resiliency and productivity in wheat.
期刊介绍:
Plant Biotechnology Journal aspires to publish original research and insightful reviews of high impact, authored by prominent researchers in applied plant science. The journal places a special emphasis on molecular plant sciences and their practical applications through plant biotechnology. Our goal is to establish a platform for showcasing significant advances in the field, encompassing curiosity-driven studies with potential applications, strategic research in plant biotechnology, scientific analysis of crucial issues for the beneficial utilization of plant sciences, and assessments of the performance of plant biotechnology products in practical applications.