OsWRKY70 在水稻抗瘟性和耐寒性中发挥着相反的作用

IF 4.8 1区 农林科学 Q1 AGRONOMY Rice Pub Date : 2024-09-14 DOI:10.1186/s12284-024-00741-9
Jiangdi Li, Yating Chen, Rui Zhang, Rujie Wang, Bin Wu, Haiwen Zhang, Guiqing Xiao
{"title":"OsWRKY70 在水稻抗瘟性和耐寒性中发挥着相反的作用","authors":"Jiangdi Li, Yating Chen, Rui Zhang, Rujie Wang, Bin Wu, Haiwen Zhang, Guiqing Xiao","doi":"10.1186/s12284-024-00741-9","DOIUrl":null,"url":null,"abstract":"<p>The transcription factor WRKYs play pivotal roles in the adapting to adverse environments in plants. Prior research has demonstrated the involvement of <i>OsWRKY70</i> in resistance against herbivores and its response to abiotic stress. Here, we reported the functional analysis of <i>OsWRKY70</i> in immunity against fungal diseases and cold tolerance. The results revealed that <i>OsWRKY70</i> was induced by various <i>Magnaporthe oryzae</i> strains. Knock out mutants of <i>OsWRKY70</i>, which were generated by the CRISPR/Cas9 system, exhibited enhanced resistance to <i>M. oryzae</i>. This was consistent with fortifying the reactive oxygen species (ROS) burst after inoculation in the mutants, elevated transcript levels of defense-responsive genes (<i>OsPR1b</i>, <i>OsPBZ1</i>, <i>OsPOX8.1</i> and <i>OsPOX22.3</i>) and the observation of the sluggish growth of invasive hyphae under fluorescence microscope. RNA sequencing (RNA-seq) and quantitative real-time PCR (qRT-PCR) validations demonstrated that differentially expressed genes were related to plant-pathogen interactions, hormone transduction and MAPK cascades. Notably, <i>OsbHLH6</i>, a key component of the JA signaling pathway, was down-regulated in the mutants compared to wild type plants. Further investigation confirmed that OsWRKY70 bound to the promoter of <i>OsbHLH6</i> by semi-in vivo chromatin immunoprecipitation (ChIP). Additionally, the loss-function of <i>OsWRKY70</i> impaired cold tolerance in rice. The enhanced susceptibility in the mutants characterized by excessive ROS production, elevated ion leakage rate and increased malondialdehyde content, as well as decreased activity of catalase (CAT) and peroxidase (POD) under low temperature stress was, which might be attributed to down-regulation of cold-responsive genes (<i>OsLti6b</i> and <i>OsICE1</i>). In conclusion, our findings indicate that OsWRKY70 negatively contributes to blast resistance but positively regulates cold tolerance in rice, providing a strategy for crop breeding with tolerance to stress.</p>","PeriodicalId":21408,"journal":{"name":"Rice","volume":null,"pages":null},"PeriodicalIF":4.8000,"publicationDate":"2024-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"OsWRKY70 Plays Opposite Roles in Blast Resistance and Cold Stress Tolerance in Rice\",\"authors\":\"Jiangdi Li, Yating Chen, Rui Zhang, Rujie Wang, Bin Wu, Haiwen Zhang, Guiqing Xiao\",\"doi\":\"10.1186/s12284-024-00741-9\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>The transcription factor WRKYs play pivotal roles in the adapting to adverse environments in plants. Prior research has demonstrated the involvement of <i>OsWRKY70</i> in resistance against herbivores and its response to abiotic stress. Here, we reported the functional analysis of <i>OsWRKY70</i> in immunity against fungal diseases and cold tolerance. The results revealed that <i>OsWRKY70</i> was induced by various <i>Magnaporthe oryzae</i> strains. Knock out mutants of <i>OsWRKY70</i>, which were generated by the CRISPR/Cas9 system, exhibited enhanced resistance to <i>M. oryzae</i>. This was consistent with fortifying the reactive oxygen species (ROS) burst after inoculation in the mutants, elevated transcript levels of defense-responsive genes (<i>OsPR1b</i>, <i>OsPBZ1</i>, <i>OsPOX8.1</i> and <i>OsPOX22.3</i>) and the observation of the sluggish growth of invasive hyphae under fluorescence microscope. RNA sequencing (RNA-seq) and quantitative real-time PCR (qRT-PCR) validations demonstrated that differentially expressed genes were related to plant-pathogen interactions, hormone transduction and MAPK cascades. Notably, <i>OsbHLH6</i>, a key component of the JA signaling pathway, was down-regulated in the mutants compared to wild type plants. Further investigation confirmed that OsWRKY70 bound to the promoter of <i>OsbHLH6</i> by semi-in vivo chromatin immunoprecipitation (ChIP). Additionally, the loss-function of <i>OsWRKY70</i> impaired cold tolerance in rice. The enhanced susceptibility in the mutants characterized by excessive ROS production, elevated ion leakage rate and increased malondialdehyde content, as well as decreased activity of catalase (CAT) and peroxidase (POD) under low temperature stress was, which might be attributed to down-regulation of cold-responsive genes (<i>OsLti6b</i> and <i>OsICE1</i>). In conclusion, our findings indicate that OsWRKY70 negatively contributes to blast resistance but positively regulates cold tolerance in rice, providing a strategy for crop breeding with tolerance to stress.</p>\",\"PeriodicalId\":21408,\"journal\":{\"name\":\"Rice\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.8000,\"publicationDate\":\"2024-09-14\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Rice\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1186/s12284-024-00741-9\",\"RegionNum\":1,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRONOMY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Rice","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1186/s12284-024-00741-9","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRONOMY","Score":null,"Total":0}
引用次数: 0

摘要

转录因子 WRKY 在植物适应不利环境方面发挥着关键作用。先前的研究表明,OsWRKY70参与了对食草动物的抵抗及其对非生物胁迫的响应。在此,我们报告了 OsWRKY70 在免疫真菌疾病和耐寒中的功能分析。结果表明,OsWRKY70可被多种Magnaporthe oryzae菌株诱导。通过CRISPR/Cas9系统生成的OsWRKY70基因敲除突变体对M.oryzae表现出更强的抗性。这与突变体接种后活性氧(ROS)爆发增强、防御响应基因(OsPR1b、OsPBZ1、OsPOX8.1 和 OsPOX22.3)转录水平升高以及在荧光显微镜下观察到侵染菌丝生长缓慢是一致的。RNA 测序(RNA-seq)和定量实时 PCR(qRT-PCR)验证表明,差异表达基因与植物与病原体相互作用、激素转导和 MAPK 级联有关。值得注意的是,与野生型植物相比,突变体中 JA 信号通路的关键成分 OsbHLH6 下调了。通过半体内染色质免疫沉淀(ChIP)进一步研究证实,OsWRKY70与OsbHLH6的启动子结合。此外,OsWRKY70的功能缺失会损害水稻的耐寒性。在低温胁迫下,突变体的易感性增强,表现为 ROS 产生过多、离子渗漏率升高、丙二醛含量增加以及过氧化氢酶(CAT)和过氧化物酶(POD)活性降低,这可能与冷响应基因(OsLti6b 和 OsICE1)的下调有关。总之,我们的研究结果表明,OsWRKY70 对水稻的抗瘟性有负作用,但对耐寒性有正向调节作用,这为作物耐逆育种提供了一种策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

摘要图片

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
OsWRKY70 Plays Opposite Roles in Blast Resistance and Cold Stress Tolerance in Rice

The transcription factor WRKYs play pivotal roles in the adapting to adverse environments in plants. Prior research has demonstrated the involvement of OsWRKY70 in resistance against herbivores and its response to abiotic stress. Here, we reported the functional analysis of OsWRKY70 in immunity against fungal diseases and cold tolerance. The results revealed that OsWRKY70 was induced by various Magnaporthe oryzae strains. Knock out mutants of OsWRKY70, which were generated by the CRISPR/Cas9 system, exhibited enhanced resistance to M. oryzae. This was consistent with fortifying the reactive oxygen species (ROS) burst after inoculation in the mutants, elevated transcript levels of defense-responsive genes (OsPR1b, OsPBZ1, OsPOX8.1 and OsPOX22.3) and the observation of the sluggish growth of invasive hyphae under fluorescence microscope. RNA sequencing (RNA-seq) and quantitative real-time PCR (qRT-PCR) validations demonstrated that differentially expressed genes were related to plant-pathogen interactions, hormone transduction and MAPK cascades. Notably, OsbHLH6, a key component of the JA signaling pathway, was down-regulated in the mutants compared to wild type plants. Further investigation confirmed that OsWRKY70 bound to the promoter of OsbHLH6 by semi-in vivo chromatin immunoprecipitation (ChIP). Additionally, the loss-function of OsWRKY70 impaired cold tolerance in rice. The enhanced susceptibility in the mutants characterized by excessive ROS production, elevated ion leakage rate and increased malondialdehyde content, as well as decreased activity of catalase (CAT) and peroxidase (POD) under low temperature stress was, which might be attributed to down-regulation of cold-responsive genes (OsLti6b and OsICE1). In conclusion, our findings indicate that OsWRKY70 negatively contributes to blast resistance but positively regulates cold tolerance in rice, providing a strategy for crop breeding with tolerance to stress.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Rice
Rice AGRONOMY-
CiteScore
10.10
自引率
3.60%
发文量
60
审稿时长
>12 weeks
期刊介绍: Rice aims to fill a glaring void in basic and applied plant science journal publishing. This journal is the world''s only high-quality serial publication for reporting current advances in rice genetics, structural and functional genomics, comparative genomics, molecular biology and physiology, molecular breeding and comparative biology. Rice welcomes review articles and original papers in all of the aforementioned areas and serves as the primary source of newly published information for researchers and students in rice and related research.
期刊最新文献
OsPIPK-FAB, A Negative Regulator in Rice Immunity Unveiled by OsMBL1 Inhibition. CRISPR-Based Modulation of uORFs in DEP1 and GIF1 for Enhanced Rice Yield Traits. Indole-3-Acetic Acid (IAA) and Sugar Mediate Endosperm Development in Rice (Oryza sativa L.). OsPP2C49, a Negative Regulatory Factor in the Abscisic Acid Signaling Pathway, Positively Regulates Grain Yield in Rice. A Cyclin Gene OsCYCB1;5 Regulates Seed Callus Induction in Rice Revealed by Genome Wide Association Study.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1