{"title":"OsWRKY70 在水稻抗瘟性和耐寒性中发挥着相反的作用","authors":"Jiangdi Li, Yating Chen, Rui Zhang, Rujie Wang, Bin Wu, Haiwen Zhang, Guiqing Xiao","doi":"10.1186/s12284-024-00741-9","DOIUrl":null,"url":null,"abstract":"<p>The transcription factor WRKYs play pivotal roles in the adapting to adverse environments in plants. Prior research has demonstrated the involvement of <i>OsWRKY70</i> in resistance against herbivores and its response to abiotic stress. Here, we reported the functional analysis of <i>OsWRKY70</i> in immunity against fungal diseases and cold tolerance. The results revealed that <i>OsWRKY70</i> was induced by various <i>Magnaporthe oryzae</i> strains. Knock out mutants of <i>OsWRKY70</i>, which were generated by the CRISPR/Cas9 system, exhibited enhanced resistance to <i>M. oryzae</i>. This was consistent with fortifying the reactive oxygen species (ROS) burst after inoculation in the mutants, elevated transcript levels of defense-responsive genes (<i>OsPR1b</i>, <i>OsPBZ1</i>, <i>OsPOX8.1</i> and <i>OsPOX22.3</i>) and the observation of the sluggish growth of invasive hyphae under fluorescence microscope. RNA sequencing (RNA-seq) and quantitative real-time PCR (qRT-PCR) validations demonstrated that differentially expressed genes were related to plant-pathogen interactions, hormone transduction and MAPK cascades. Notably, <i>OsbHLH6</i>, a key component of the JA signaling pathway, was down-regulated in the mutants compared to wild type plants. Further investigation confirmed that OsWRKY70 bound to the promoter of <i>OsbHLH6</i> by semi-in vivo chromatin immunoprecipitation (ChIP). Additionally, the loss-function of <i>OsWRKY70</i> impaired cold tolerance in rice. The enhanced susceptibility in the mutants characterized by excessive ROS production, elevated ion leakage rate and increased malondialdehyde content, as well as decreased activity of catalase (CAT) and peroxidase (POD) under low temperature stress was, which might be attributed to down-regulation of cold-responsive genes (<i>OsLti6b</i> and <i>OsICE1</i>). In conclusion, our findings indicate that OsWRKY70 negatively contributes to blast resistance but positively regulates cold tolerance in rice, providing a strategy for crop breeding with tolerance to stress.</p>","PeriodicalId":21408,"journal":{"name":"Rice","volume":null,"pages":null},"PeriodicalIF":4.8000,"publicationDate":"2024-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"OsWRKY70 Plays Opposite Roles in Blast Resistance and Cold Stress Tolerance in Rice\",\"authors\":\"Jiangdi Li, Yating Chen, Rui Zhang, Rujie Wang, Bin Wu, Haiwen Zhang, Guiqing Xiao\",\"doi\":\"10.1186/s12284-024-00741-9\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>The transcription factor WRKYs play pivotal roles in the adapting to adverse environments in plants. Prior research has demonstrated the involvement of <i>OsWRKY70</i> in resistance against herbivores and its response to abiotic stress. Here, we reported the functional analysis of <i>OsWRKY70</i> in immunity against fungal diseases and cold tolerance. The results revealed that <i>OsWRKY70</i> was induced by various <i>Magnaporthe oryzae</i> strains. Knock out mutants of <i>OsWRKY70</i>, which were generated by the CRISPR/Cas9 system, exhibited enhanced resistance to <i>M. oryzae</i>. This was consistent with fortifying the reactive oxygen species (ROS) burst after inoculation in the mutants, elevated transcript levels of defense-responsive genes (<i>OsPR1b</i>, <i>OsPBZ1</i>, <i>OsPOX8.1</i> and <i>OsPOX22.3</i>) and the observation of the sluggish growth of invasive hyphae under fluorescence microscope. RNA sequencing (RNA-seq) and quantitative real-time PCR (qRT-PCR) validations demonstrated that differentially expressed genes were related to plant-pathogen interactions, hormone transduction and MAPK cascades. Notably, <i>OsbHLH6</i>, a key component of the JA signaling pathway, was down-regulated in the mutants compared to wild type plants. Further investigation confirmed that OsWRKY70 bound to the promoter of <i>OsbHLH6</i> by semi-in vivo chromatin immunoprecipitation (ChIP). Additionally, the loss-function of <i>OsWRKY70</i> impaired cold tolerance in rice. The enhanced susceptibility in the mutants characterized by excessive ROS production, elevated ion leakage rate and increased malondialdehyde content, as well as decreased activity of catalase (CAT) and peroxidase (POD) under low temperature stress was, which might be attributed to down-regulation of cold-responsive genes (<i>OsLti6b</i> and <i>OsICE1</i>). In conclusion, our findings indicate that OsWRKY70 negatively contributes to blast resistance but positively regulates cold tolerance in rice, providing a strategy for crop breeding with tolerance to stress.</p>\",\"PeriodicalId\":21408,\"journal\":{\"name\":\"Rice\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.8000,\"publicationDate\":\"2024-09-14\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Rice\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1186/s12284-024-00741-9\",\"RegionNum\":1,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRONOMY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Rice","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1186/s12284-024-00741-9","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRONOMY","Score":null,"Total":0}
OsWRKY70 Plays Opposite Roles in Blast Resistance and Cold Stress Tolerance in Rice
The transcription factor WRKYs play pivotal roles in the adapting to adverse environments in plants. Prior research has demonstrated the involvement of OsWRKY70 in resistance against herbivores and its response to abiotic stress. Here, we reported the functional analysis of OsWRKY70 in immunity against fungal diseases and cold tolerance. The results revealed that OsWRKY70 was induced by various Magnaporthe oryzae strains. Knock out mutants of OsWRKY70, which were generated by the CRISPR/Cas9 system, exhibited enhanced resistance to M. oryzae. This was consistent with fortifying the reactive oxygen species (ROS) burst after inoculation in the mutants, elevated transcript levels of defense-responsive genes (OsPR1b, OsPBZ1, OsPOX8.1 and OsPOX22.3) and the observation of the sluggish growth of invasive hyphae under fluorescence microscope. RNA sequencing (RNA-seq) and quantitative real-time PCR (qRT-PCR) validations demonstrated that differentially expressed genes were related to plant-pathogen interactions, hormone transduction and MAPK cascades. Notably, OsbHLH6, a key component of the JA signaling pathway, was down-regulated in the mutants compared to wild type plants. Further investigation confirmed that OsWRKY70 bound to the promoter of OsbHLH6 by semi-in vivo chromatin immunoprecipitation (ChIP). Additionally, the loss-function of OsWRKY70 impaired cold tolerance in rice. The enhanced susceptibility in the mutants characterized by excessive ROS production, elevated ion leakage rate and increased malondialdehyde content, as well as decreased activity of catalase (CAT) and peroxidase (POD) under low temperature stress was, which might be attributed to down-regulation of cold-responsive genes (OsLti6b and OsICE1). In conclusion, our findings indicate that OsWRKY70 negatively contributes to blast resistance but positively regulates cold tolerance in rice, providing a strategy for crop breeding with tolerance to stress.
期刊介绍:
Rice aims to fill a glaring void in basic and applied plant science journal publishing. This journal is the world''s only high-quality serial publication for reporting current advances in rice genetics, structural and functional genomics, comparative genomics, molecular biology and physiology, molecular breeding and comparative biology. Rice welcomes review articles and original papers in all of the aforementioned areas and serves as the primary source of newly published information for researchers and students in rice and related research.