从玉米麦芽中提取的酵素对木薯粉(Manihot esculenta Crantz)消化率的影响

IF 3.1 4区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Applied Biochemistry and Biotechnology Pub Date : 2024-09-18 DOI:10.1007/s12010-024-05044-9
Arthur K. Amisi, Erick A. Chimanuka, Guelor L. Kasereka, Roger V. Kizungu, Jean-Claude T. Bwanganga
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引用次数: 0

摘要

本研究旨在确定从玉米麦芽中提取的酶对木薯淀粉消化率的影响,这将成为现代酿酒厂将当地产品融入酿酒工艺问题的答案之一。不同浓度的木薯淀粉溶液(E0: 0 g/L;E1: 1 g/L;E2: 1.1 g/L;E3:制备了不同浓度的木薯淀粉溶液(E0:0/L;E1:1 g/L;E2:1.1 g/L;E3:1.2 g/L;E4:1.3 g/L;E5:1.4 g/L;E6:1.5 g/L),并对其进行了两种处理(糊化和非糊化),在试管中各放置 5 mL。然后在每个装有木薯粉溶液的试管中加入三毫升(3 mL)含有从麦芽玉米中提取的淀粉酶的溶液。所有处理均经过三个温度阶段(50 ℃ 15 分钟、90 ℃ 20 分钟和 100 ℃ 75 分钟)。采用完全因子设计(2 个处理 × 2 个温度水平)对 28 个对象(两个重复)进行了实验。结果表明,糊化没有影响,这可能是由于玉米酶活性的最适温度较高。浓度也没有显著差异,这表明这些浓度可以很好地用于工业规模,用玉米麦芽酶消化木薯淀粉。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Effect of the Digestibility of Cassava Flour (Manihot esculenta Crantz) by Enzymes Extracted from Corn Malt

The aim of this study is to determine the effect of the digestibility of cassava starch by the enzymes extracted from corn malt, which will constitute one of the answers to the problem of integrating local products into the process in a modern brewery. Cassava starch solutions of different concentrations (E0: 0 g/L; E1: 1 g/L; E2: 1.1 g/L; E3: 1.2 g/L; E4: 1.3 g/L; E5: 1.4 g/L and E6: 1.5 g/L) were prepared and subjected to two treatments (gelatinized and non-gelatinized) and 5 mL of each were placed in a test tube. Three millilitres (3 mL) of the solution containing amylases extracted from malt corn was then added to each of the test tubes containing the cassava flour solutions. All the treatments were subjected to three temperature stages (50 °C for 15 min, 90 °C for 20 min, and 100 °C for 75 min). Twenty-eight (28) objects (two duplicates) were experimented in a complete factorial design (2 treatments × 2 temperature levels). The results obtained showed that gelatinization had no effect, which could be due to the high optimum temperatures of corn enzyme activity. The concentrations also did not have significant differences which shows that these concentrations can well be used on an industrial scale to digest cassava starch by corn malt enzymes.

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来源期刊
Applied Biochemistry and Biotechnology
Applied Biochemistry and Biotechnology 工程技术-生化与分子生物学
CiteScore
5.70
自引率
6.70%
发文量
460
审稿时长
5.3 months
期刊介绍: This journal is devoted to publishing the highest quality innovative papers in the fields of biochemistry and biotechnology. The typical focus of the journal is to report applications of novel scientific and technological breakthroughs, as well as technological subjects that are still in the proof-of-concept stage. Applied Biochemistry and Biotechnology provides a forum for case studies and practical concepts of biotechnology, utilization, including controls, statistical data analysis, problem descriptions unique to a particular application, and bioprocess economic analyses. The journal publishes reviews deemed of interest to readers, as well as book reviews, meeting and symposia notices, and news items relating to biotechnology in both the industrial and academic communities. In addition, Applied Biochemistry and Biotechnology often publishes lists of patents and publications of special interest to readers.
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