针对血管免疫母细胞性 T 细胞淋巴瘤的体内 CAR T 细胞疗法

IF 11.4 1区 医学 Q1 ONCOLOGY Journal of Experimental & Clinical Cancer Research Pub Date : 2024-09-14 DOI:10.1186/s13046-024-03179-5
Adrien Krug, Aymen Saidane, Chiara Martinello, Floriane Fusil, Alexander Michels, Christian J. Buchholz, Jean-Ehrland Ricci, Els Verhoeyen
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摘要

血管免疫母细胞T细胞淋巴瘤(AITL)是一种罕见的癌症,目前尚无特效治疗方法,生存率很低。我们之前开发了一种血管免疫母细胞淋巴瘤小鼠模型,该模型与人类疾病非常相似,可用于评估新疗法。与人类 AITL 一样,小鼠 CD4+ 滤泡辅助性 T(Tfh)细胞是恶性肿瘤的驱动因素。因此,嵌合抗原受体(CAR)T细胞疗法可能是一种新的治疗选择。为了在递送 CD4 特异性 CAR 时防止 CAR T 细胞自相残杀,我们使用了编码抗 CD4 CAR 的慢病毒载体 (LV),使其能独家进入 CD8 T 细胞。在小鼠 AITL 活检中,这些抗 CD4CAR CD8 靶向 LV 在 CD8 T 细胞中实现了高 CAR 表达水平。恶性 CD4 Tfh 细胞从 mAITL 淋巴瘤中被清除,而 CAR + CD8 T 细胞在与 CD4 受体相遇后得到扩增,并形成功能性细胞毒性细胞。最后,将 CAR + CD8-LVs 体内注射到携带淋巴瘤的临床前 AITL 小鼠模型中,可显著延长小鼠的存活时间。此外,体内生成的功能性 CAR + CD8 T 细胞有效减少了 mAITL 肿瘤中的肿瘤性 T 细胞数量。这是首次描述体内生成的 CAR T 细胞用于治疗 T 细胞淋巴瘤。所述策略为 CD4 驱动的 T 细胞淋巴瘤患者提供了一种新的治疗理念。
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In vivo CAR T cell therapy against angioimmunoblastic T cell lymphoma
For angioimmunoblastic T cell lymphoma (AITL), a rare cancer, no specific treatments are available and survival outcome is poor. We previously developed a murine model for AITL that mimics closely human disease and allows to evaluate new treatments. As in human AITL, the murine CD4+ follicular helper T (Tfh) cells are drivers of the malignancy. Therefore, chimeric antigen receptor (CAR) T cell therapy might represent a new therapeutic option. To prevent fratricide among CAR T cells when delivering an CD4-specific CAR, we used a lentiviral vector (LV) encoding an anti-CD4 CAR, allowing exclusive entry into CD8 T cells. These anti-CD4CAR CD8-targeted LVs achieved in murine AITL biopsies high CAR-expression levels in CD8 T cells. Malignant CD4 Tfh cells were eliminated from the mAITL lymphoma, while the CAR + CD8 T cells expanded upon encounter with the CD4 receptor and were shaped into functional cytotoxic cells. Finally, in vivo injection of the CAR + CD8-LVs into our preclinical AITL mouse model carrying lymphomas, significantly prolonged mice survival. Moreover, the in vivo generated functional CAR + CD8 T cells efficiently reduced neoplastic T cell numbers in the mAITL tumors. This is the first description of in vivo generated CAR T cells for therapy of a T cell lymphoma. The strategy described offers a new therapeutic concept for patients suffering from CD4-driven T cell lymphomas.
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来源期刊
CiteScore
18.20
自引率
1.80%
发文量
333
审稿时长
1 months
期刊介绍: The Journal of Experimental & Clinical Cancer Research is an esteemed peer-reviewed publication that focuses on cancer research, encompassing everything from fundamental discoveries to practical applications. We welcome submissions that showcase groundbreaking advancements in the field of cancer research, especially those that bridge the gap between laboratory findings and clinical implementation. Our goal is to foster a deeper understanding of cancer, improve prevention and detection strategies, facilitate accurate diagnosis, and enhance treatment options. We are particularly interested in manuscripts that shed light on the mechanisms behind the development and progression of cancer, including metastasis. Additionally, we encourage submissions that explore molecular alterations or biomarkers that can help predict the efficacy of different treatments or identify drug resistance. Translational research related to targeted therapies, personalized medicine, tumor immunotherapy, and innovative approaches applicable to clinical investigations are also of great interest to us. We provide a platform for the dissemination of large-scale molecular characterizations of human tumors and encourage researchers to share their insights, discoveries, and methodologies with the wider scientific community. By publishing high-quality research articles, reviews, and commentaries, the Journal of Experimental & Clinical Cancer Research strives to contribute to the continuous improvement of cancer care and make a meaningful impact on patients' lives.
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