{"title":"体外条件下硼酸对狐狸葡萄(Vitis Labrusca L.)耐盐性的影响","authors":"Sümeyra Akden, Hatice Bilir Ekbic, Mert Ilhan","doi":"10.1007/s10341-024-01182-w","DOIUrl":null,"url":null,"abstract":"<p>Shoots obtained from micro-cuttings of ‘Balıkçı Siyahı’ (<i>Vitis labrusca</i> L.) grape type were used as explants. The explants were cultivated in MS nutrient medium containing 1 mg/l benzyl adenine for shoot formation following surface sterilization. Shoots derived from the explants were transferred to MS medium containing 2 mg/l indole-3-butyric acid for rooting. The rooted plantlets were transferred to MS medium containing four doses (0, 0.5, 1, 2 mM) of H<sub>3</sub>BO<sub>3</sub> and three doses (0, 100, 200 mM) of NaCl for determination of salt stress and the effectiveness of boric acid. In the study, some physiological parameters such as plant vitality (%), damage degree (0–3), some shoot growth parameters, chlorophyll content (SPAD), shoot tolerance ratio, leaf turgor weight (g), ion flux (%), cell membrane damage ratio (%) and explant relative water content (%) were evaluated. As salt doses increase, it has been observed that the damage, ion flux and cell membrane damage rate also increase. It has been determined that the most negative effect occurs in the plant with the application of 200 mM NaCl, significantly reducing growth and development. Overall, it has been determined that 0.5 mM and 1 mM H<sub>3</sub>BO<sub>3</sub> doses reduce the negative effects caused by salt stress.</p>","PeriodicalId":11889,"journal":{"name":"Erwerbs-Obstbau","volume":"5 1","pages":""},"PeriodicalIF":1.2000,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Effect of Boric Acid in In Vitro Conditions on the Salt Tolerance of Fox Grapes (Vitis Labrusca L.)\",\"authors\":\"Sümeyra Akden, Hatice Bilir Ekbic, Mert Ilhan\",\"doi\":\"10.1007/s10341-024-01182-w\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Shoots obtained from micro-cuttings of ‘Balıkçı Siyahı’ (<i>Vitis labrusca</i> L.) grape type were used as explants. The explants were cultivated in MS nutrient medium containing 1 mg/l benzyl adenine for shoot formation following surface sterilization. Shoots derived from the explants were transferred to MS medium containing 2 mg/l indole-3-butyric acid for rooting. The rooted plantlets were transferred to MS medium containing four doses (0, 0.5, 1, 2 mM) of H<sub>3</sub>BO<sub>3</sub> and three doses (0, 100, 200 mM) of NaCl for determination of salt stress and the effectiveness of boric acid. In the study, some physiological parameters such as plant vitality (%), damage degree (0–3), some shoot growth parameters, chlorophyll content (SPAD), shoot tolerance ratio, leaf turgor weight (g), ion flux (%), cell membrane damage ratio (%) and explant relative water content (%) were evaluated. As salt doses increase, it has been observed that the damage, ion flux and cell membrane damage rate also increase. It has been determined that the most negative effect occurs in the plant with the application of 200 mM NaCl, significantly reducing growth and development. Overall, it has been determined that 0.5 mM and 1 mM H<sub>3</sub>BO<sub>3</sub> doses reduce the negative effects caused by salt stress.</p>\",\"PeriodicalId\":11889,\"journal\":{\"name\":\"Erwerbs-Obstbau\",\"volume\":\"5 1\",\"pages\":\"\"},\"PeriodicalIF\":1.2000,\"publicationDate\":\"2024-09-13\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Erwerbs-Obstbau\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1007/s10341-024-01182-w\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"HORTICULTURE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Erwerbs-Obstbau","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1007/s10341-024-01182-w","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"HORTICULTURE","Score":null,"Total":0}
引用次数: 0
摘要
从 "Balıkçı Siyahı"(Vitis labrusca L.)葡萄微切口获得的嫩枝被用作外植体。外植体在含有 1 mg/l 苄基腺嘌呤的 MS 营养培养基中培养,经表面消毒后形成芽。将外植体产生的芽转移到含 2 毫克/升吲哚-3-丁酸的 MS 培养基中生根。将生根的小植株转移到含有四种剂量(0、0.5、1、2 mM)H3BO3 和三种剂量(0、100、200 mM)NaCl 的 MS 培养基中,以测定盐胁迫和硼酸的有效性。研究评估了一些生理参数,如植株活力(%)、损伤程度(0-3)、一些芽生长参数、叶绿素含量(SPAD)、芽耐受率、叶片张力重量(克)、离子通量(%)、细胞膜损伤率(%)和外植体相对含水量(%)。据观察,随着盐剂量的增加,损伤率、离子通量和细胞膜损伤率也随之增加。据测定,施用 200 毫摩尔 NaCl 对植物的负面影响最大,会显著降低生长和发育。总之,0.5 毫摩尔和 1 毫摩尔的 H3BO3 剂量可减少盐胁迫造成的负面影响。
Effect of Boric Acid in In Vitro Conditions on the Salt Tolerance of Fox Grapes (Vitis Labrusca L.)
Shoots obtained from micro-cuttings of ‘Balıkçı Siyahı’ (Vitis labrusca L.) grape type were used as explants. The explants were cultivated in MS nutrient medium containing 1 mg/l benzyl adenine for shoot formation following surface sterilization. Shoots derived from the explants were transferred to MS medium containing 2 mg/l indole-3-butyric acid for rooting. The rooted plantlets were transferred to MS medium containing four doses (0, 0.5, 1, 2 mM) of H3BO3 and three doses (0, 100, 200 mM) of NaCl for determination of salt stress and the effectiveness of boric acid. In the study, some physiological parameters such as plant vitality (%), damage degree (0–3), some shoot growth parameters, chlorophyll content (SPAD), shoot tolerance ratio, leaf turgor weight (g), ion flux (%), cell membrane damage ratio (%) and explant relative water content (%) were evaluated. As salt doses increase, it has been observed that the damage, ion flux and cell membrane damage rate also increase. It has been determined that the most negative effect occurs in the plant with the application of 200 mM NaCl, significantly reducing growth and development. Overall, it has been determined that 0.5 mM and 1 mM H3BO3 doses reduce the negative effects caused by salt stress.
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