去污剂诱导脂质囊泡融合的多个中间体

IF 7.5 Q1 MATERIALS SCIENCE, MULTIDISCIPLINARY Communications Materials Pub Date : 2024-09-19 DOI:10.1038/s43246-024-00628-8
Lara G. Dresser, Casper Kunstmann-Olsen, Donato Conteduca, Christopher M. Hofmair, Nathan Smith, Laura Clark, Steven Johnson, J. Carlos Penedo, Mark C. Leake, Steven D. Quinn
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引用次数: 0

摘要

去垢剂诱导的囊泡相互作用对病毒灭活等应用至关重要,这种相互作用因去垢剂类型和膜组成而异,但其基本机理细节仍未得到充分探索。在这里,我们利用基于佛斯特共振能量转移(FRET)的脂质混合测定法和单囊泡表征方法,确定亚微米大小的囊泡在非离子型洗涤剂 Triton-X-100 的诱导下发生融合。我们证明了这一过程是一个多步骤机制,其特点是膜嵌入荧光团之间的 FRET 效率值离散,涉及渗透、囊泡对接、半融合以及在亚溶解洗涤剂浓度下的全脂混合。我们还利用无标记石英晶体微天平与耗散监测方法剖析了囊泡与表面系留囊泡融合的动力学,为生物技术应用开辟了一个平台。所介绍的策略从机理上揭示了囊泡融合的动力学,对药物输送和传感器开发等应用具有重要意义,因为在这些应用中,封装货物的输送和操控至关重要。
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Multiple intermediates in the detergent-induced fusion of lipid vesicles
Detergent-induced vesicle interactions, critical for applications including virus inactivation, varies according to the detergent type and membrane composition, but the underlying mechanistic details remain underexplored. Here, we use a lipid mixing assay based on Förster resonance energy transfer (FRET), and single-vesicle characterization approaches to identify that sub-micron-sized vesicles are induced to fuse by the non-ionic detergent Triton-X-100. We demonstrate that the process is a multi-step mechanism, characterized by discrete values of FRET efficiency between membrane-embedded fluorophores, and involves permeabilization, vesicle docking, hemi-fusion and full lipid mixing at sub-solubilizing detergent concentrations. We also dissect the kinetics of vesicle fusion to surface-tethered vesicles using a label-free quartz-crystal microbalance with dissipation monitoring approach, opening a platform for biotechnology applications. The presented strategies provide mechanistic insight into the dynamics of vesicle fusion and have implications for applications including drug delivery and sensor development where transport and manipulation of encapsulated cargo is essential. Detergent-induced membrane interactions are important for biotechnology applications but their mechanism is still not well understood. Here, sub-micron-sized vesicles are shown to fuse by a non-ionic detergent, involving permeabilization, vesicle docking, hemi-fusion, and full lipid mixing steps.
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来源期刊
Communications Materials
Communications Materials MATERIALS SCIENCE, MULTIDISCIPLINARY-
CiteScore
12.10
自引率
1.30%
发文量
85
审稿时长
17 weeks
期刊介绍: Communications Materials, a selective open access journal within Nature Portfolio, is dedicated to publishing top-tier research, reviews, and commentary across all facets of materials science. The journal showcases significant advancements in specialized research areas, encompassing both fundamental and applied studies. Serving as an open access option for materials sciences, Communications Materials applies less stringent criteria for impact and significance compared to Nature-branded journals, including Nature Communications.
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