{"title":"用于选择性检测水介质中次氯酸盐和活细胞成像的近红外荧光开启探针","authors":"Anwesha Maiti, Saikat Kumar Manna, Satyajit Halder, Rajdeep Ganguly, Anirban Karak, Pintu Ghosh, Kuladip Jana, Ajit Kumar Mahapatra","doi":"10.1021/acs.chemrestox.4c00222","DOIUrl":null,"url":null,"abstract":"Hypochlorite, as an important reactive oxygen species (ROS), plays a vital role in many physiological and pathological processes, but an excess concentration of hypochlorite (ClO<sup>–</sup>) may become toxic to humans and cause disease. Hence, the selective and rapid detection of hypochlorite (ClO<sup>–</sup>) is necessary for human safety. Here, we report a novel near-infrared (NIR) fluorescence “turn-on” and highly selective benzophenoxazinium chloride-based fluorescent probe, <b>BPH</b> (benzophenoxazinium dihydroxy benzaldehyde), for hypochlorite detection. Due to hypochlorite-induced vicinal diol oxidation to the corresponding ortho benzoquinone derivative, the photoinduced electron transfer (PET) process, which was operating from vicinal diol to the benzophenoxazinium chloride receptor moiety, was suddenly inhibited, as a result of which strong NIR fluorescence “turn-on” emission was observed. The detection limit of <b>BPH</b> was found to be 2.39 × 10<sup>–10</sup> M, or 0.23 nM. <b>BPH</b> was successfully applied for exogenous and endogenous hypochlorite detection in live MDA-MB 231 cells.","PeriodicalId":31,"journal":{"name":"Chemical Research in Toxicology","volume":null,"pages":null},"PeriodicalIF":3.7000,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Near-Infrared Fluorescent Turn-On Probe for Selective Detection of Hypochlorite in Aqueous Medium and Live Cell Imaging\",\"authors\":\"Anwesha Maiti, Saikat Kumar Manna, Satyajit Halder, Rajdeep Ganguly, Anirban Karak, Pintu Ghosh, Kuladip Jana, Ajit Kumar Mahapatra\",\"doi\":\"10.1021/acs.chemrestox.4c00222\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Hypochlorite, as an important reactive oxygen species (ROS), plays a vital role in many physiological and pathological processes, but an excess concentration of hypochlorite (ClO<sup>–</sup>) may become toxic to humans and cause disease. Hence, the selective and rapid detection of hypochlorite (ClO<sup>–</sup>) is necessary for human safety. Here, we report a novel near-infrared (NIR) fluorescence “turn-on” and highly selective benzophenoxazinium chloride-based fluorescent probe, <b>BPH</b> (benzophenoxazinium dihydroxy benzaldehyde), for hypochlorite detection. Due to hypochlorite-induced vicinal diol oxidation to the corresponding ortho benzoquinone derivative, the photoinduced electron transfer (PET) process, which was operating from vicinal diol to the benzophenoxazinium chloride receptor moiety, was suddenly inhibited, as a result of which strong NIR fluorescence “turn-on” emission was observed. The detection limit of <b>BPH</b> was found to be 2.39 × 10<sup>–10</sup> M, or 0.23 nM. <b>BPH</b> was successfully applied for exogenous and endogenous hypochlorite detection in live MDA-MB 231 cells.\",\"PeriodicalId\":31,\"journal\":{\"name\":\"Chemical Research in Toxicology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.7000,\"publicationDate\":\"2024-09-17\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Chemical Research in Toxicology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1021/acs.chemrestox.4c00222\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"CHEMISTRY, MEDICINAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chemical Research in Toxicology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1021/acs.chemrestox.4c00222","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, MEDICINAL","Score":null,"Total":0}
Near-Infrared Fluorescent Turn-On Probe for Selective Detection of Hypochlorite in Aqueous Medium and Live Cell Imaging
Hypochlorite, as an important reactive oxygen species (ROS), plays a vital role in many physiological and pathological processes, but an excess concentration of hypochlorite (ClO–) may become toxic to humans and cause disease. Hence, the selective and rapid detection of hypochlorite (ClO–) is necessary for human safety. Here, we report a novel near-infrared (NIR) fluorescence “turn-on” and highly selective benzophenoxazinium chloride-based fluorescent probe, BPH (benzophenoxazinium dihydroxy benzaldehyde), for hypochlorite detection. Due to hypochlorite-induced vicinal diol oxidation to the corresponding ortho benzoquinone derivative, the photoinduced electron transfer (PET) process, which was operating from vicinal diol to the benzophenoxazinium chloride receptor moiety, was suddenly inhibited, as a result of which strong NIR fluorescence “turn-on” emission was observed. The detection limit of BPH was found to be 2.39 × 10–10 M, or 0.23 nM. BPH was successfully applied for exogenous and endogenous hypochlorite detection in live MDA-MB 231 cells.
期刊介绍：
Chemical Research in Toxicology publishes Articles, Rapid Reports, Chemical Profiles, Reviews, Perspectives, Letters to the Editor, and ToxWatch on a wide range of topics in Toxicology that inform a chemical and molecular understanding and capacity to predict biological outcomes on the basis of structures and processes. The overarching goal of activities reported in the Journal are to provide knowledge and innovative approaches needed to promote intelligent solutions for human safety and ecosystem preservation. The journal emphasizes insight concerning mechanisms of toxicity over phenomenological observations. It upholds rigorous chemical, physical and mathematical standards for characterization and application of modern techniques.
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