Aparana Kumari, B. Stany, Shatakshi Mishra, Ridhima Biswas, Angad Bakshi, B. Abhrajit, K. V. Bhaskara Rao
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引用次数: 0
摘要
放线菌是生物活性化合物的重要生产者。这些化合物具有多种有益特性,如抗氧化、消炎、抗癌、抗菌和抗病毒等。温泉具有高温和独特的地球化学条件,是孕育多种微生物生命的迷人生态系统。在这些环境中,放线菌利用现有的有机物和营养物质茁壮成长。以高温和 pH 值为特征的极端条件可能会产生选择性压力,有利于放线菌等生命力顽强的微生物生存。本研究特别发现了放线菌属细菌(Genbank ID:OP863023),它是从拉吉尔温泉中分离出来的,在 43 °C的温度下能产生一种值得注意的棕色色素。通过气相色谱-质谱(GC-MS)分析发现,该分离菌株可产生多种代谢物。在对所有代谢物进行 ADME/T 筛选后,邻苯二甲酸二乙酯(DEP)在放线菌属细菌产生的代谢物中脱颖而出。分离出的菌株具有体外抗菌活性,对单核细胞增生李斯特菌 MTCC1143 的抑制区为 10 毫米,而参考氨苄西林盘的抑制区为 15 毫米。在针对单核细胞增生李斯特氏菌的内部蛋白 A 蛋白进行的硅学分析中,放线菌属生产的化合物 DEP 显示出-5.4 千卡/摩尔的抑制潜能,而参考药物氨苄西林则显示出-7.4 千卡/摩尔的更高抑制潜能。这些发现表明,分离出的作用菌有望用于抗菌,尤其是对单核细胞增生性酵母菌。
Investigation of antibacterial potential of Actinomycetales isolated from hot springs in India through a combined analysis involving in vitro and computational methods
Actinobacteria emerge as significant producers of bioactive compounds. These compounds exhibit diverse beneficial properties, such as antioxidative, anti-inflammatory, anticancer, antimicrobial, and antiviral attributes. Hot springs, with their high temperatures and unique geochemical conditions, serve as fascinating ecosystems harboring diverse microbial life. In these environments, actinomycetes thrive, utilizing the available organic matter and nutrients. The extreme conditions, characterized by high temperatures and pH levels, likely exert selective pressure, favoring the survival of resilient microorganisms like actinomycetes. This study specifically identified Actinomycetalesbacterium spp. (Genbank ID: OP863023), isolated from Rajgir hot spring, as a noteworthy producer of a brown pigment at 43 °C. This isolated strain is known to produce various metabolites identified through GC–MS analysis. Following ADME/T screening of all the metabolites, Diethyl Phthalate (DEP) stands out among those produced by the Actinomycetales bacterium species. The isolated strain demonstrated in vitro antibacterial activity, inhibiting Listeria monocytogenes MTCC1143 with a 10-mm zone of inhibition, while the reference Ampicillin disc showed a 15 mm inhibition zone. In the in silico analysis targeting the Internalin A protein of L. monocytogenes, the Actinomycetales-produced compound DEP displayed an inhibition potential of − 5.4 kcal/Mol, while the reference drug Ampicillin exhibited a higher inhibition potential of − 7.4 kcal/Mol. These findings suggest that the isolated actionobacteria holds promise for antibacterial applications, particularly against L. monocytogenes.
Chemical PapersChemical Engineering-General Chemical Engineering
CiteScore
3.30
自引率
4.50%
发文量
590
期刊介绍:
Chemical Papers is a peer-reviewed, international journal devoted to basic and applied chemical research. It has a broad scope covering the chemical sciences, but favors interdisciplinary research and studies that bring chemistry together with other disciplines.