通过体外和计算方法的综合分析,研究从印度温泉中分离出来的放线菌的抗菌潜力

IF 2.2 4区 化学 Q2 Engineering Chemical Papers Pub Date : 2024-09-18 DOI:10.1007/s11696-024-03689-8
Aparana Kumari, B. Stany, Shatakshi Mishra, Ridhima Biswas, Angad Bakshi, B. Abhrajit, K. V. Bhaskara Rao
{"title":"通过体外和计算方法的综合分析,研究从印度温泉中分离出来的放线菌的抗菌潜力","authors":"Aparana Kumari,&nbsp;B. Stany,&nbsp;Shatakshi Mishra,&nbsp;Ridhima Biswas,&nbsp;Angad Bakshi,&nbsp;B. Abhrajit,&nbsp;K. V. Bhaskara Rao","doi":"10.1007/s11696-024-03689-8","DOIUrl":null,"url":null,"abstract":"<div><p>Actinobacteria emerge as significant producers of bioactive compounds. These compounds exhibit diverse beneficial properties, such as antioxidative, anti-inflammatory, anticancer, antimicrobial, and antiviral attributes. Hot springs, with their high temperatures and unique geochemical conditions, serve as fascinating ecosystems harboring diverse microbial life. In these environments, actinomycetes thrive, utilizing the available organic matter and nutrients. The extreme conditions, characterized by high temperatures and pH levels, likely exert selective pressure, favoring the survival of resilient microorganisms like actinomycetes. This study specifically identified <i>Actinomycetales</i> <i>bacterium</i> spp. (Genbank ID: OP863023), isolated from Rajgir hot spring, as a noteworthy producer of a brown pigment at 43 °C. This isolated strain is known to produce various metabolites identified through GC–MS analysis. Following ADME/T screening of all the metabolites, Diethyl Phthalate (DEP) stands out among those produced by the <i>Actinomycetales</i> bacterium species. The isolated strain demonstrated in vitro antibacterial activity, inhibiting <i>Listeria monocytogenes</i> MTCC1143 with a 10-mm zone of inhibition, while the reference Ampicillin disc showed a 15 mm inhibition zone. In the in silico analysis targeting the Internalin A protein of <i>L. monocytogenes</i>, the <i>Actinomycetales-</i>produced compound DEP displayed an inhibition potential of − 5.4 kcal/Mol, while the reference drug Ampicillin exhibited a higher inhibition potential of − 7.4 kcal/Mol. These findings suggest that the isolated actionobacteria holds promise for antibacterial applications, particularly against <i>L. monocytogenes</i>.</p></div>","PeriodicalId":513,"journal":{"name":"Chemical Papers","volume":null,"pages":null},"PeriodicalIF":2.2000,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Investigation of antibacterial potential of Actinomycetales isolated from hot springs in India through a combined analysis involving in vitro and computational methods\",\"authors\":\"Aparana Kumari,&nbsp;B. Stany,&nbsp;Shatakshi Mishra,&nbsp;Ridhima Biswas,&nbsp;Angad Bakshi,&nbsp;B. Abhrajit,&nbsp;K. V. Bhaskara Rao\",\"doi\":\"10.1007/s11696-024-03689-8\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Actinobacteria emerge as significant producers of bioactive compounds. These compounds exhibit diverse beneficial properties, such as antioxidative, anti-inflammatory, anticancer, antimicrobial, and antiviral attributes. Hot springs, with their high temperatures and unique geochemical conditions, serve as fascinating ecosystems harboring diverse microbial life. In these environments, actinomycetes thrive, utilizing the available organic matter and nutrients. The extreme conditions, characterized by high temperatures and pH levels, likely exert selective pressure, favoring the survival of resilient microorganisms like actinomycetes. This study specifically identified <i>Actinomycetales</i> <i>bacterium</i> spp. (Genbank ID: OP863023), isolated from Rajgir hot spring, as a noteworthy producer of a brown pigment at 43 °C. This isolated strain is known to produce various metabolites identified through GC–MS analysis. Following ADME/T screening of all the metabolites, Diethyl Phthalate (DEP) stands out among those produced by the <i>Actinomycetales</i> bacterium species. The isolated strain demonstrated in vitro antibacterial activity, inhibiting <i>Listeria monocytogenes</i> MTCC1143 with a 10-mm zone of inhibition, while the reference Ampicillin disc showed a 15 mm inhibition zone. In the in silico analysis targeting the Internalin A protein of <i>L. monocytogenes</i>, the <i>Actinomycetales-</i>produced compound DEP displayed an inhibition potential of − 5.4 kcal/Mol, while the reference drug Ampicillin exhibited a higher inhibition potential of − 7.4 kcal/Mol. These findings suggest that the isolated actionobacteria holds promise for antibacterial applications, particularly against <i>L. monocytogenes</i>.</p></div>\",\"PeriodicalId\":513,\"journal\":{\"name\":\"Chemical Papers\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.2000,\"publicationDate\":\"2024-09-18\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Chemical Papers\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s11696-024-03689-8\",\"RegionNum\":4,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"Engineering\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chemical Papers","FirstCategoryId":"92","ListUrlMain":"https://link.springer.com/article/10.1007/s11696-024-03689-8","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"Engineering","Score":null,"Total":0}
引用次数: 0

摘要

放线菌是生物活性化合物的重要生产者。这些化合物具有多种有益特性,如抗氧化、消炎、抗癌、抗菌和抗病毒等。温泉具有高温和独特的地球化学条件,是孕育多种微生物生命的迷人生态系统。在这些环境中,放线菌利用现有的有机物和营养物质茁壮成长。以高温和 pH 值为特征的极端条件可能会产生选择性压力,有利于放线菌等生命力顽强的微生物生存。本研究特别发现了放线菌属细菌(Genbank ID:OP863023),它是从拉吉尔温泉中分离出来的,在 43 °C的温度下能产生一种值得注意的棕色色素。通过气相色谱-质谱(GC-MS)分析发现,该分离菌株可产生多种代谢物。在对所有代谢物进行 ADME/T 筛选后,邻苯二甲酸二乙酯(DEP)在放线菌属细菌产生的代谢物中脱颖而出。分离出的菌株具有体外抗菌活性,对单核细胞增生李斯特菌 MTCC1143 的抑制区为 10 毫米,而参考氨苄西林盘的抑制区为 15 毫米。在针对单核细胞增生李斯特氏菌的内部蛋白 A 蛋白进行的硅学分析中,放线菌属生产的化合物 DEP 显示出-5.4 千卡/摩尔的抑制潜能,而参考药物氨苄西林则显示出-7.4 千卡/摩尔的更高抑制潜能。这些发现表明,分离出的作用菌有望用于抗菌,尤其是对单核细胞增生性酵母菌。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

摘要图片

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Investigation of antibacterial potential of Actinomycetales isolated from hot springs in India through a combined analysis involving in vitro and computational methods

Actinobacteria emerge as significant producers of bioactive compounds. These compounds exhibit diverse beneficial properties, such as antioxidative, anti-inflammatory, anticancer, antimicrobial, and antiviral attributes. Hot springs, with their high temperatures and unique geochemical conditions, serve as fascinating ecosystems harboring diverse microbial life. In these environments, actinomycetes thrive, utilizing the available organic matter and nutrients. The extreme conditions, characterized by high temperatures and pH levels, likely exert selective pressure, favoring the survival of resilient microorganisms like actinomycetes. This study specifically identified Actinomycetales bacterium spp. (Genbank ID: OP863023), isolated from Rajgir hot spring, as a noteworthy producer of a brown pigment at 43 °C. This isolated strain is known to produce various metabolites identified through GC–MS analysis. Following ADME/T screening of all the metabolites, Diethyl Phthalate (DEP) stands out among those produced by the Actinomycetales bacterium species. The isolated strain demonstrated in vitro antibacterial activity, inhibiting Listeria monocytogenes MTCC1143 with a 10-mm zone of inhibition, while the reference Ampicillin disc showed a 15 mm inhibition zone. In the in silico analysis targeting the Internalin A protein of L. monocytogenes, the Actinomycetales-produced compound DEP displayed an inhibition potential of − 5.4 kcal/Mol, while the reference drug Ampicillin exhibited a higher inhibition potential of − 7.4 kcal/Mol. These findings suggest that the isolated actionobacteria holds promise for antibacterial applications, particularly against L. monocytogenes.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Chemical Papers
Chemical Papers Chemical Engineering-General Chemical Engineering
CiteScore
3.30
自引率
4.50%
发文量
590
期刊介绍: Chemical Papers is a peer-reviewed, international journal devoted to basic and applied chemical research. It has a broad scope covering the chemical sciences, but favors interdisciplinary research and studies that bring chemistry together with other disciplines.
期刊最新文献
Chalky limestone as sustainable water purification from eriochrome black T: kinetics, isotherm, and equilibrium O-Allyloxy chalcone derivatives: design, synthesis, anticancer activity, network pharmacology and molecular docking Natural and synthetic polymers as effective corrosion inhibitors: a concise review Development of molecularly imprinted polymer-based dispersive micro-solid-phase extraction for the selective extraction of histamine from canned tuna fish samples prior to its determination by GC–FID Virtual screening, docking, molecular dynamics study of efflux pump inhibitors against Helicobacter pylori
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1