橡胶树病原体 Rigidoporus microporus 分泌效应组的硅学分析凸显了其潜在的毒力蛋白

IF 4 2区 生物学 Q2 MICROBIOLOGY Frontiers in Microbiology Pub Date : 2024-09-16 DOI:10.3389/fmicb.2024.1439454
Rawit Longsaward, Unchera Viboonjun, Zilan Wen, Fred O. Asiegbu
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引用次数: 0

摘要

橡胶树白色根腐病的致病菌小孢子虫(Rigidoporus microporus)对全世界的天然橡胶生产构成了重大威胁。了解促进其致病性的分子机制对于制定有效的疾病管理策略至关重要。病原体分泌的效应蛋白在调节宿主免疫反应和感染方面发挥着关键作用。在这项研究中,我们从小孢子虫基因组中发现了 357 种推测的分泌效应蛋白。然后将这些蛋白与之前获得的橡胶树乳胶暴露反应的 RNA-seq 数据进行整合。对推测效应蛋白进行的注释表明,与小孢子菌毒力相关的几个家族中的蛋白含量很高,尤其是疏水蛋白和糖苷水解酶(GH)蛋白。讨论了分泌型效应物对真菌致病性的贡献,尤其是对橡胶树胶乳暴露的反应。对一些未知的高表达效应物的蛋白质结构进行了预测,发现它们与氨基肽酶、泛素连接酶、球蛋白和haumatin蛋白相似。这项综合研究进一步阐明了小孢子菌致病的分子机制,并为制定橡胶园白根腐病的防治策略提供了替代目标。
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In silico analysis of secreted effectorome of the rubber tree pathogen Rigidoporus microporus highlights its potential virulence proteins
Rigidoporus microporus, the causative agent of the white root rot disease of rubber trees, poses a significant threat to natural rubber production worldwide. Understanding the molecular mechanisms facilitating its pathogenicity would be crucial for developing effective disease management strategies. The pathogen secretes effector proteins, which play pivotal roles in modulating host immune responses and infection. In this study, in silico analyses identified 357 putative secreted effector proteins from the R. microporus genome. These were then integrated into previous RNA-seq data obtained in response to rubber tree latex exposure. Annotation of putative effectors suggested the abundance of proteins in several families associated with the virulence of R. microporus, especially hydrophobin proteins and glycoside hydrolase (GH) proteins. The contribution of secreted effectors to fungal pathogenicity was discussed, particularly in response to rubber tree latex exposure. Some unknown highly expressed effectors were predicted for the protein structures, revealing their similarity to aminopeptidase, ubiquitin ligase, spherulin, and thaumatin protein. This integrative study further elucidates the molecular mechanism of R. microporus pathogenesis and offers alternative targets for developing control strategies for managing white root rot disease in rubber plantations.
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来源期刊
CiteScore
7.70
自引率
9.60%
发文量
4837
审稿时长
14 weeks
期刊介绍: Frontiers in Microbiology is a leading journal in its field, publishing rigorously peer-reviewed research across the entire spectrum of microbiology. Field Chief Editor Martin G. Klotz at Washington State University is supported by an outstanding Editorial Board of international researchers. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to researchers, academics, clinicians and the public worldwide.
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