木材白腐菌 Lenzites gibbosa 热休克基因 Lghsp17.4 的特征和表达

IF 3.4 2区 农林科学 Q1 FORESTRY Journal of Forestry Research Pub Date : 2024-09-15 DOI:10.1007/s11676-024-01778-8
Lianrong Feng, Yujie Chi, Jian Zhang, Xuxin Yang, Shuying Han
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引用次数: 0

摘要

小热休克蛋白(sHSPs)是一种分子伴侣蛋白,可防止非生物胁迫(尤其是热休克)过程中受损蛋白质的积累,但其作用机制尚不清楚。L. gibbosa菌丝体在25 °C下生长9 d,在33 °C下处理15、30、60和120 min,然后进行转录组测序。从筛选出的 32 个热休克蛋白(HSP)基因中,克隆出一个高表达基因,命名为 Lghsp17.4。利用 RT-qPCR 分析了 Lghsp17.4 基因在热休克和染料胁迫下的表达情况。结果表明,两种处理都能在转录水平上诱导 Lghsp17.4 的高表达,表明 Lghsp17.4 可能在热胁迫和染料降解的响应中起作用。之前我们发现,L. gibbosa在降解芳香族化合物的过程中通常会发生热休克反应(HSR),而HSP总是与锰过氧化物酶(MnPs)和其他木质素降解酶一起产生。因此,我们测定了33 °C热冲击后吉勃菌中MnPs的活性,以分析MnPs表达与Lghsp17.4表达之间的关系。0-30分钟的热冲击增加了MnPs的活性,而MnPs活性的变化与Lghsp17.4的表达水平密切正相关,这表明LgHSP17.4与MnPs之间在长臂猿HSR过程中存在潜在的联系和相互作用。因此,LgHSP17.4 可能对长臂猿的 HSR 有积极的调节作用,是抗逆机制的一个重要组成部分。
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Characteristics and expression of heat shock gene Lghsp17.4 in Lenzites gibbosa, a white rot fungus of wood

Small heat shock proteins (sHSPs) act as molecular chaperones that can prevent the accumulation of damaged proteins during abiotic stress, especially heat shock, but the mechanism is not clear. To study the function of sHSPs in Lenzites gibbosa, a common polypore in northern temperate forests that causes spongy white rot of broadleaf trees, under temperature stress, L. gibbosa mycelia were grown at 25 °C for 9 d, treated at 33 °C for 15, 30, 60, and 120 min before sequencing the transcriptomes. From among 32 heat shock protein (HSP) genes found in the screen of the transcriptome data, a highly expressed gene was cloned and named Lghsp17.4. RT-qPCR was used to analyze the expression of the gene Lghsp17.4 under heat shock and dye stress. Both treatments induced higher expression of Lghsp17.4 at the transcriptional level, indicating that Lghsp17.4 might function in the response to heat stress and dye degradation. We previously found that L. gibbosa generally had a heat shock reaction (HSR) during degradation of aromatic compounds, and HSPs were always produced with manganese peroxidases (MnPs) and other lignin-degrading enzymes. Therefore, we measured the activity of MnPs in L. gibbosa after 33 °C heat shock to analyze the relationship between MnPs expression and Lghsp17.4 expression. Heat shocks of 0–30 min increased MnPs activity, and the change in MnPs activity were closely positively correlated with the expression levels of Lghsp17.4 over time, indicating a potential connection and interaction between LgHSP17.4 and MnPs during the HSR in L. gibbosa. Thus, LgHSP17.4 might have a positive regulatory effect on the HSR in L. gibbosa and be a critical component of a stress resistance mechanism.

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来源期刊
CiteScore
7.30
自引率
3.30%
发文量
2538
期刊介绍: The Journal of Forestry Research (JFR), founded in 1990, is a peer-reviewed quarterly journal in English. JFR has rapidly emerged as an international journal published by Northeast Forestry University and Ecological Society of China in collaboration with Springer Verlag. The journal publishes scientific articles related to forestry for a broad range of international scientists, forest managers and practitioners.The scope of the journal covers the following five thematic categories and 20 subjects: Basic Science of Forestry, Forest biometrics, Forest soils, Forest hydrology, Tree physiology, Forest biomass, carbon, and bioenergy, Forest biotechnology and molecular biology, Forest Ecology, Forest ecology, Forest ecological services, Restoration ecology, Forest adaptation to climate change, Wildlife ecology and management, Silviculture and Forest Management, Forest genetics and tree breeding, Silviculture, Forest RS, GIS, and modeling, Forest management, Forest Protection, Forest entomology and pathology, Forest fire, Forest resources conservation, Forest health monitoring and assessment, Wood Science and Technology, Wood Science and Technology.
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Applying palaeoecological analogues to contemporary challenges: community-level effects of canopy gaps caused by systematic decline of a prevalent tree species A stacking-based model for the spread of Botryosphaeria laricina Leaf functional traits and ecological strategies of common plant species in evergreen broad-leaved forests on Huangshan Mountain Characteristics and expression of heat shock gene Lghsp17.4 in Lenzites gibbosa, a white rot fungus of wood Tree diversity drives understory carbon storage rather than overstory carbon storage across forest types
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