Lianrong Feng, Yujie Chi, Jian Zhang, Xuxin Yang, Shuying Han
{"title":"木材白腐菌 Lenzites gibbosa 热休克基因 Lghsp17.4 的特征和表达","authors":"Lianrong Feng, Yujie Chi, Jian Zhang, Xuxin Yang, Shuying Han","doi":"10.1007/s11676-024-01778-8","DOIUrl":null,"url":null,"abstract":"<p>Small heat shock proteins (sHSPs) act as molecular chaperones that can prevent the accumulation of damaged proteins during abiotic stress, especially heat shock, but the mechanism is not clear. To study the function of sHSPs in <i>Lenzites gibbosa</i>, a common polypore in northern temperate forests that causes spongy white rot of broadleaf trees, under temperature stress, <i>L. gibbosa</i> mycelia were grown at 25 °C for 9 d, treated at 33 °C for 15, 30, 60, and 120 min before sequencing the transcriptomes. From among 32 heat shock protein (HSP) genes found in the screen of the transcriptome data, a highly expressed gene was cloned and named <i>Lghsp17.4</i>. RT-qPCR was used to analyze the expression of the gene <i>Lghsp17.4</i> under heat shock and dye stress. Both treatments induced higher expression of <i>Lghsp17.4</i> at the transcriptional level, indicating that <i>Lghsp17.4</i> might function in the response to heat stress and dye degradation. We previously found that <i>L. gibbosa</i> generally had a heat shock reaction (HSR) during degradation of aromatic compounds, and HSPs were always produced with manganese peroxidases (MnPs) and other lignin-degrading enzymes. Therefore, we measured the activity of MnPs in <i>L. gibbosa</i> after 33 °C heat shock to analyze the relationship between MnPs expression and <i>Lghsp17.4</i> expression. Heat shocks of 0–30 min increased MnPs activity, and the change in MnPs activity were closely positively correlated with the expression levels of <i>Lghsp17.4</i> over time, indicating a potential connection and interaction between LgHSP17.4 and MnPs during the HSR in <i>L. gibbosa</i>. Thus, LgHSP17.4 might have a positive regulatory effect on the HSR in <i>L. gibbosa</i> and be a critical component of a stress resistance mechanism.</p>","PeriodicalId":15830,"journal":{"name":"Journal of Forestry Research","volume":null,"pages":null},"PeriodicalIF":3.4000,"publicationDate":"2024-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Characteristics and expression of heat shock gene Lghsp17.4 in Lenzites gibbosa, a white rot fungus of wood\",\"authors\":\"Lianrong Feng, Yujie Chi, Jian Zhang, Xuxin Yang, Shuying Han\",\"doi\":\"10.1007/s11676-024-01778-8\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Small heat shock proteins (sHSPs) act as molecular chaperones that can prevent the accumulation of damaged proteins during abiotic stress, especially heat shock, but the mechanism is not clear. To study the function of sHSPs in <i>Lenzites gibbosa</i>, a common polypore in northern temperate forests that causes spongy white rot of broadleaf trees, under temperature stress, <i>L. gibbosa</i> mycelia were grown at 25 °C for 9 d, treated at 33 °C for 15, 30, 60, and 120 min before sequencing the transcriptomes. From among 32 heat shock protein (HSP) genes found in the screen of the transcriptome data, a highly expressed gene was cloned and named <i>Lghsp17.4</i>. RT-qPCR was used to analyze the expression of the gene <i>Lghsp17.4</i> under heat shock and dye stress. Both treatments induced higher expression of <i>Lghsp17.4</i> at the transcriptional level, indicating that <i>Lghsp17.4</i> might function in the response to heat stress and dye degradation. We previously found that <i>L. gibbosa</i> generally had a heat shock reaction (HSR) during degradation of aromatic compounds, and HSPs were always produced with manganese peroxidases (MnPs) and other lignin-degrading enzymes. Therefore, we measured the activity of MnPs in <i>L. gibbosa</i> after 33 °C heat shock to analyze the relationship between MnPs expression and <i>Lghsp17.4</i> expression. Heat shocks of 0–30 min increased MnPs activity, and the change in MnPs activity were closely positively correlated with the expression levels of <i>Lghsp17.4</i> over time, indicating a potential connection and interaction between LgHSP17.4 and MnPs during the HSR in <i>L. gibbosa</i>. Thus, LgHSP17.4 might have a positive regulatory effect on the HSR in <i>L. gibbosa</i> and be a critical component of a stress resistance mechanism.</p>\",\"PeriodicalId\":15830,\"journal\":{\"name\":\"Journal of Forestry Research\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.4000,\"publicationDate\":\"2024-09-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Forestry Research\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1007/s11676-024-01778-8\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"FORESTRY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Forestry Research","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1007/s11676-024-01778-8","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"FORESTRY","Score":null,"Total":0}
Characteristics and expression of heat shock gene Lghsp17.4 in Lenzites gibbosa, a white rot fungus of wood
Small heat shock proteins (sHSPs) act as molecular chaperones that can prevent the accumulation of damaged proteins during abiotic stress, especially heat shock, but the mechanism is not clear. To study the function of sHSPs in Lenzites gibbosa, a common polypore in northern temperate forests that causes spongy white rot of broadleaf trees, under temperature stress, L. gibbosa mycelia were grown at 25 °C for 9 d, treated at 33 °C for 15, 30, 60, and 120 min before sequencing the transcriptomes. From among 32 heat shock protein (HSP) genes found in the screen of the transcriptome data, a highly expressed gene was cloned and named Lghsp17.4. RT-qPCR was used to analyze the expression of the gene Lghsp17.4 under heat shock and dye stress. Both treatments induced higher expression of Lghsp17.4 at the transcriptional level, indicating that Lghsp17.4 might function in the response to heat stress and dye degradation. We previously found that L. gibbosa generally had a heat shock reaction (HSR) during degradation of aromatic compounds, and HSPs were always produced with manganese peroxidases (MnPs) and other lignin-degrading enzymes. Therefore, we measured the activity of MnPs in L. gibbosa after 33 °C heat shock to analyze the relationship between MnPs expression and Lghsp17.4 expression. Heat shocks of 0–30 min increased MnPs activity, and the change in MnPs activity were closely positively correlated with the expression levels of Lghsp17.4 over time, indicating a potential connection and interaction between LgHSP17.4 and MnPs during the HSR in L. gibbosa. Thus, LgHSP17.4 might have a positive regulatory effect on the HSR in L. gibbosa and be a critical component of a stress resistance mechanism.
期刊介绍:
The Journal of Forestry Research (JFR), founded in 1990, is a peer-reviewed quarterly journal in English. JFR has rapidly emerged as an international journal published by Northeast Forestry University and Ecological Society of China in collaboration with Springer Verlag. The journal publishes scientific articles related to forestry for a broad range of international scientists, forest managers and practitioners.The scope of the journal covers the following five thematic categories and 20 subjects:
Basic Science of Forestry,
Forest biometrics,
Forest soils,
Forest hydrology,
Tree physiology,
Forest biomass, carbon, and bioenergy,
Forest biotechnology and molecular biology,
Forest Ecology,
Forest ecology,
Forest ecological services,
Restoration ecology,
Forest adaptation to climate change,
Wildlife ecology and management,
Silviculture and Forest Management,
Forest genetics and tree breeding,
Silviculture,
Forest RS, GIS, and modeling,
Forest management,
Forest Protection,
Forest entomology and pathology,
Forest fire,
Forest resources conservation,
Forest health monitoring and assessment,
Wood Science and Technology,
Wood Science and Technology.