980纳米二极管激光照射对脐带间充质干细胞增殖的影响

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS ACS Applied Bio Materials Pub Date : 2024-09-16 DOI:10.1016/j.tice.2024.102568
Zahra Al Timimi
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引用次数: 0

摘要

使用低强度激光辐射可以加速各种细胞的生长。这项研究旨在观察低能量强度的激光辐射对从脐带产生的间充质干细胞(MSCs)在低营养条件下增殖和存活能力的影响。研究使用了两种不同的能量密度,即 2.5 j/cm2 和 5 j/cm2,并使用了 980 nm 的二极管激光辐射。这样就能在不同浓度(7.5%、10%、12.5% 和 15%)的胎牛血清受控环境中观察这些特定元素对细胞行为的影响。细胞在缺乏营养的培养基中生长,并添加了不同数量的胎牛血清。MTT 试验用于评估细胞的线粒体活性。72 小时后,结果显示,经 2.5 j/cm2 和 10 % 胎牛血清处理的细胞的 MTT 试验活性明显高于经 5 j/cm2 处理的细胞。
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The impact of 980nm diode laser irradiation on the proliferation of mesenchymal stem cells derived from the umbilical cord's

Various cell types can have their growth accelerated by using low-intensity laser radiation. The study is intended to look at the impacts of laser radiation at low energy intensity on the ability of mesenchymal stem cells (MSCs) generated from umbilical cords to proliferate as well as survive in low-nutrient conditions. The study applied two different energy densities, 2.5 j/cm2 and 5 j/cm2, using a 980 nm diode laser radiation. This allowed for the observation of the effects of these specific elements on the behavior of the cells in a controlled environment at various concentrations of fetal bovine (7.5 %, 10 %, 12.5 %, and 15 %). The cells were grown in a medium lacking in nutrients and were enriched with varying quantities of serum from fetal bovines. The MTT test was used to evaluate the mitochondrial activity of the cell. Following 72 hours, it was shown that cells treated with 2.5 j/cm2 and 10 % fetal bovine serum had significantly higher MTT test activity than cells treated with 5 j/cm2.The results of this study show that even in the presence of dietary deficiencies, low-intensity laser radiation therapy can stimulate the growth of mesenchymal stem cells isolated from umbilical cords.

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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
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