{"title":"有机肥而非化学肥料通过调节高地红壤中相关微生物的功能特性,提高了暗二氧化碳的固定。","authors":"Qian Wang, Mengmeng Liu, Jingshi Huang, Cheng Han, Yunbin Jiang, Huan Deng, Kailou Liu, Wenhui Zhong","doi":"10.1016/j.scitotenv.2024.176337","DOIUrl":null,"url":null,"abstract":"<p><p>Dark microbial fixation of CO<sub>2</sub> is an indispensable process for soil carbon sequestration. However, the whole genetic information involved in dark CO<sub>2</sub> fixation and its influence on dark CO<sub>2</sub> fixation rates under diversified fertilization regimes were largely unclear. Here, revealed by <sup>13</sup>C-CO<sub>2</sub> labeling, dark CO<sub>2</sub> fixation rates in upland red soils ranged from 0.029 mg kg<sup>-1</sup> d<sup>-1</sup> to 0.092 mg kg<sup>-1</sup> d<sup>-1</sup>, and it was 75.49 % higher (P < 0.05) in organic manure (OM) soil but 44.2 % decline (P < 0.05) in chemical nitrogen fertilizer (N) soil compared to unfertilized (CK) soil. In addition, the normalized abundance and Chao1 index of dark CO<sub>2</sub> fixation genes (KO level) were significantly different between OM and N soils, showing the highest and lowest, respectively. And they were positively (P < 0.05) correlated with dark CO<sub>2</sub> fixation rate. Besides, among the identified CO<sub>2</sub> fixation pathways in this study, the DC/4-HB cycle (M00374) was enriched in OM soil, yet the 3-HP cycle (M00376) was enriched in N soil, and their relative abundances were positively and negatively correlated (P < 0.05) with dark CO<sub>2</sub> fixation rate, respectively. The PLS-SEM analysis revealed that dark CO<sub>2</sub> fixation-related functional traits (i.e. normalized abundance, Chao1 index and gene composition) were directly and positively associated with dark CO<sub>2</sub> fixation rate, and organic manure could exert a positive effect on soil dark CO<sub>2</sub> fixation rate through enhancing soil properties (e.g., pH and soil organic carbon) and further altering associated microbial functional traits. These results have implications for explaining and predicting the soil CO<sub>2</sub> fixation process from the perspective of microbial functional potential.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":null,"pages":null},"PeriodicalIF":5.4000,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Organic manure rather than chemical fertilization improved dark CO<sub>2</sub> fixation by regulating associated microbial functional traits in upland red soils.\",\"authors\":\"Qian Wang, Mengmeng Liu, Jingshi Huang, Cheng Han, Yunbin Jiang, Huan Deng, Kailou Liu, Wenhui Zhong\",\"doi\":\"10.1016/j.scitotenv.2024.176337\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Dark microbial fixation of CO<sub>2</sub> is an indispensable process for soil carbon sequestration. However, the whole genetic information involved in dark CO<sub>2</sub> fixation and its influence on dark CO<sub>2</sub> fixation rates under diversified fertilization regimes were largely unclear. Here, revealed by <sup>13</sup>C-CO<sub>2</sub> labeling, dark CO<sub>2</sub> fixation rates in upland red soils ranged from 0.029 mg kg<sup>-1</sup> d<sup>-1</sup> to 0.092 mg kg<sup>-1</sup> d<sup>-1</sup>, and it was 75.49 % higher (P < 0.05) in organic manure (OM) soil but 44.2 % decline (P < 0.05) in chemical nitrogen fertilizer (N) soil compared to unfertilized (CK) soil. In addition, the normalized abundance and Chao1 index of dark CO<sub>2</sub> fixation genes (KO level) were significantly different between OM and N soils, showing the highest and lowest, respectively. And they were positively (P < 0.05) correlated with dark CO<sub>2</sub> fixation rate. Besides, among the identified CO<sub>2</sub> fixation pathways in this study, the DC/4-HB cycle (M00374) was enriched in OM soil, yet the 3-HP cycle (M00376) was enriched in N soil, and their relative abundances were positively and negatively correlated (P < 0.05) with dark CO<sub>2</sub> fixation rate, respectively. The PLS-SEM analysis revealed that dark CO<sub>2</sub> fixation-related functional traits (i.e. normalized abundance, Chao1 index and gene composition) were directly and positively associated with dark CO<sub>2</sub> fixation rate, and organic manure could exert a positive effect on soil dark CO<sub>2</sub> fixation rate through enhancing soil properties (e.g., pH and soil organic carbon) and further altering associated microbial functional traits. These results have implications for explaining and predicting the soil CO<sub>2</sub> fixation process from the perspective of microbial functional potential.</p>\",\"PeriodicalId\":8,\"journal\":{\"name\":\"ACS Biomaterials Science & Engineering\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":5.4000,\"publicationDate\":\"2024-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"ACS Biomaterials Science & Engineering\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://doi.org/10.1016/j.scitotenv.2024.176337\",\"RegionNum\":2,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/9/19 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q2\",\"JCRName\":\"MATERIALS SCIENCE, BIOMATERIALS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Biomaterials Science & Engineering","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1016/j.scitotenv.2024.176337","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/9/19 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"MATERIALS SCIENCE, BIOMATERIALS","Score":null,"Total":0}
引用次数: 0
摘要
微生物暗固定二氧化碳是土壤固碳不可或缺的过程。然而,在不同施肥制度下,暗固定二氧化碳的整个遗传信息及其对暗固定二氧化碳速率的影响尚不清楚。本文通过 13C-CO2 标记揭示,高地红壤的暗 CO2 固定率介于 0.029 mg kg-1 d-1 至 0.092 mg kg-1 d-1 之间,且在 OM 土和 N 土之间有显著差异,分别最高和最低(P 2 固定基因(KO 水平)在 OM 土和 N 土之间有显著差异,分别最高和最低),且两者呈正相关(P 2 固定基因(KO 水平)在 OM 土和 N 土之间有显著差异,分别最高和最低)。它们与 P2 固定率呈正相关。此外,在本研究确定的二氧化碳固定途径中,DC/4-HB 循环(M00374)在 OM 土壤中富集,而 3-HP 循环(M00376)在 N 土壤中富集,它们的相对丰度分别与 P 2 固定率呈正相关和负相关。PLS-SEM分析表明,CO2暗固定相关功能性状(即归一化丰度、Chao1指数和基因组成)与CO2暗固定率直接正相关,有机肥可通过提高土壤性质(如pH值和土壤有机碳)和进一步改变相关微生物功能性状对土壤CO2暗固定率产生积极影响。这些结果对于从微生物功能潜力的角度解释和预测土壤二氧化碳固定过程具有重要意义。
Organic manure rather than chemical fertilization improved dark CO2 fixation by regulating associated microbial functional traits in upland red soils.
Dark microbial fixation of CO2 is an indispensable process for soil carbon sequestration. However, the whole genetic information involved in dark CO2 fixation and its influence on dark CO2 fixation rates under diversified fertilization regimes were largely unclear. Here, revealed by 13C-CO2 labeling, dark CO2 fixation rates in upland red soils ranged from 0.029 mg kg-1 d-1 to 0.092 mg kg-1 d-1, and it was 75.49 % higher (P < 0.05) in organic manure (OM) soil but 44.2 % decline (P < 0.05) in chemical nitrogen fertilizer (N) soil compared to unfertilized (CK) soil. In addition, the normalized abundance and Chao1 index of dark CO2 fixation genes (KO level) were significantly different between OM and N soils, showing the highest and lowest, respectively. And they were positively (P < 0.05) correlated with dark CO2 fixation rate. Besides, among the identified CO2 fixation pathways in this study, the DC/4-HB cycle (M00374) was enriched in OM soil, yet the 3-HP cycle (M00376) was enriched in N soil, and their relative abundances were positively and negatively correlated (P < 0.05) with dark CO2 fixation rate, respectively. The PLS-SEM analysis revealed that dark CO2 fixation-related functional traits (i.e. normalized abundance, Chao1 index and gene composition) were directly and positively associated with dark CO2 fixation rate, and organic manure could exert a positive effect on soil dark CO2 fixation rate through enhancing soil properties (e.g., pH and soil organic carbon) and further altering associated microbial functional traits. These results have implications for explaining and predicting the soil CO2 fixation process from the perspective of microbial functional potential.
期刊介绍:
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