一种基于 Fe3O4/AuNPs-MWCNTs@PDA 纳米复合材料的电化学免疫传感器，用于灵敏快速地检测胱抑素 C。

IF 2.6 4区生物学 Q2 BIOCHEMICAL RESEARCH METHODS Analytical biochemistry Pub Date : 2024-09-21 DOI:10.1016/j.ab.2024.115677

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引用次数: 0

摘要

血清胱抑素 C（CysC）是早期诊断肾功能不全的重要标志物。在这项工作中，我们建立了一种基于 Fe3O4/AuNPs-MWCNTs@PDA 纳米复合材料的新型电化学免疫传感器，用于检测 CysC。通过多巴胺封装的 Fe3O4/AuNPs-MWCNTs@PDA 纳米酶复合物不仅能携带大量检测抗体，还能通过静电吸附结合电活性物质甲苯胺蓝（TB）。通过将 AuNPs 固定在电极上以结合捕获抗体（Ab1），我们构建了一种低成本、高灵敏度和可重复性的夹心电化学免疫传感器。其检测范围为 3.9-125.0 纳克/毫升，电流峰值差（ip）与 CysC 浓度的对数之间存在显著的线性关系。此外，该免疫传感器的检测限为 0.157 纳克/毫升。我们成功地利用这种新型免疫传感器检测了人体血清样本中的 CysC，这些结果对其在临床应用中的潜在用途具有重要意义。

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An electrochemical immunosensor for sensitive and rapid detection of cystatin C based on Fe3O4/AuNPs-MWCNTs@PDA nanocomposite

Serum Cystatin C (CysC) is an impressive marker for early diagnosis of renal dysfunction. In this work, we established a novel electrochemical immunosensor based on Fe₃O₄/AuNPs-MWCNTs@PDA nanocomposite for the detection of CysC. The Fe₃O₄/AuNPs-MWCNTs@PDA nanozyme complex by polydopamine encapsulation can not only carry massive detection antibodies, but also bind the electroactive substance toluidine blue (TB) through electrostatic adsorption. By immobilizing AuNPs onto the electrode to bind the capture antibody (Ab1), we constructed a sandwich electrochemical immunosensor with low cost, high sensitivity, and repeatability. The detection range is 3.9–125.0 ng/mL with a significant linear relationship between the current peak difference (ip) and logarithm of the CysC concentration. Moreover, the detection limit of the immunosensor is 0.157 ng/mL. We have successfully utilized this novel immunosensor to detect CysC in human serum samples, and these results have implications for its potential use in clinical application.

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来源期刊

Analytical biochemistry 生物-分析化学

CiteScore

5.70

自引率

0.00%

发文量

283

审稿时长

44 days

期刊介绍： The journal''s title Analytical Biochemistry: Methods in the Biological Sciences declares its broad scope: methods for the basic biological sciences that include biochemistry, molecular genetics, cell biology, proteomics, immunology, bioinformatics and wherever the frontiers of research take the field. The emphasis is on methods from the strictly analytical to the more preparative that would include novel approaches to protein purification as well as improvements in cell and organ culture. The actual techniques are equally inclusive ranging from aptamers to zymology. The journal has been particularly active in: -Analytical techniques for biological molecules- Aptamer selection and utilization- Biosensors- Chromatography- Cloning, sequencing and mutagenesis- Electrochemical methods- Electrophoresis- Enzyme characterization methods- Immunological approaches- Mass spectrometry of proteins and nucleic acids- Metabolomics- Nano level techniques- Optical spectroscopy in all its forms. The journal is reluctant to include most drug and strictly clinical studies as there are more suitable publication platforms for these types of papers.