波兰刺猬冠状病毒的全基因组特征及其物种间传播潜力的尖峰蛋白进化分析。

IF 2.3 2区 农林科学 Q1 VETERINARY SCIENCES BMC Veterinary Research Pub Date : 2024-09-21 DOI:10.1186/s12917-024-04277-4
Katarzyna Domanska-Blicharz, Anna Lisowska, Justyna Opolska, Jakub J Ruszkowski, Maciej Gogulski, Małgorzata Pomorska-Mól
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引用次数: 0

摘要

背景:刺猬最近被确认为中东呼吸综合征冠状病毒(MERS-CoV-like)的可能贮藏地。这些病毒被归类为MerBCoV-Eriirus亚属中一个独特的Betacoronavirus erinacei(BCoV-Eri)种。由于冠状病毒具有在不同宿主(包括人类)之间跳跃的能力,这可能会对与刺猬直接接触的人群(如动物收容所的工作人员)造成特别的威胁。我们之前的研究表明,在野生动物康复中心收集的动物中存在 BCoV-Eri 株系。本研究旨在调查随后从波兰城市地区收集的刺猬中是否存在冠状病毒及其分子特征:结果:对刺猬冠状病毒感染情况的监测发现了五只阳性个体。共有 20% 的受测动物感染了 BCoV-Eri。我们的分析表明,CoVs 阳性与动物健康状况之间没有关联,但幼年刺猬和雌性刺猬感染这种病毒的几率更高。我们对两株波兰刺猬冠状病毒1的全基因组进行了测序,并将其与欧洲和亚洲国家的同种病毒进行了比较。系统发育分析表明,这两种CoV毒株与其他类似的MerBCoV-Eriirus形成了共同的群集,但也发现它们在遗传上存在变异,而且大多数变化都发生在S蛋白上。我们的分析表明,刺猬冠状病毒 1 株系的一些 S 蛋白位点是在正选择压力下进化而来的,在五个这样的位点中,有三个位于刺猬冠状病毒 1 株系的 S1 区,另外两个位于刺猬冠状病毒 1 株系的 S2 区:结论:BCoV-Eri 在波兰的野生动物收容所有一定程度的流行。鉴于 BCoVs-Eri 的 S 蛋白变化很大,而且该蛋白的某些位点在正选择压力下进化,这些菌株有可能获得跨物种传播的有利特征。因此,在这些收容所工作的人类面临的威胁尤其大。因此,充分的生物安全保障措施以及人类对此类风险的认识至关重要。
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Whole genome characteristics of hedgehog coronaviruses from Poland and analysis of the evolution of the Spike protein for its interspecies transmission potential.

Background: The hedgehogs have been recently identified as possible reservoir of Middle East respiratory syndrome coronavirus like (MERS-CoV-like). These viruses were classified as a distinct Betacoronavirus erinacei (BCoV-Eri) species within the MerBCoV-Eriirus subgenus. As coronaviruses are known for their ability to jump between different hosts, including humans, this can pose a particular threat to people in direct contact with hedgehogs, such as those working at animal asylums. Our previous studies have shown the presence of BCoV-Eri strains in animals collected in the wildlife rehabilitation centre. This study aimed to investigate the presence of CoV in subsequent hedgehogs collected from the urban area of Poland and their molecular characteristics.

Results: Monitoring for the presence of coronavirus infection in hedgehogs revealed five positive individuals. The presence of BCoV-Eri was found in a total of 20% of animals tested. Our analyses revealed no correlation between CoVs positivity and animal health conditions but a higher probability of such infection in juveniles and females. The whole genome of two Polish Hedgehog coronavirus 1 strains were sequenced and compared with available counterparts from European and Asian countries. Phylogenetic analysis showed that both CoV strains formed common cluster with other similar MerBCoV-Eriirus, but they were also found to be genetically variable and most changes in the S protein were identified. Our analysis revealed that some S protein sites of the Hedgehog coronavirus 1 strains evolved under positive selection pressure and of five such sites, three are in the S1 region while the other two in the S2 region of the Spike.

Conclusions: BCoV-Eri is to some extent prevalent in wildlife asylums in Poland. Given that the S protein of BCoVs-Eri is highly variable and that some sites of this protein evolve under positive selection pressure, these strains could potentially acquire a favourable feature for cross-species transmission. Consequently, the threat to humans working in such asylums is particularly high. Adequate biosecurity safeguards, but also human awareness of such risks, are therefore essential.

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来源期刊
BMC Veterinary Research
BMC Veterinary Research VETERINARY SCIENCES-
CiteScore
4.80
自引率
3.80%
发文量
420
审稿时长
3-6 weeks
期刊介绍: BMC Veterinary Research is an open access, peer-reviewed journal that considers articles on all aspects of veterinary science and medicine, including the epidemiology, diagnosis, prevention and treatment of medical conditions of domestic, companion, farm and wild animals, as well as the biomedical processes that underlie their health.
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