对用于自动透射电子显微镜网格染色的开源三维打印机 HistoEnder 进行改装。

IF 0.6 4区生物学 Q4 CELL BIOLOGY Journal of Histotechnology Pub Date : 2024-09-23 DOI:10.1080/01478885.2024.2403872

Philip Seifert

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引用次数: 0

摘要

HistoEnder是一款廉价的开源3D打印机，作为自动组织学载玻片染色机发布，已被改装用于传统的生物透射电子显微镜（TEM）批量网格染色。详细介绍了三维打印设备、组装、G 代码编程以及在三维打印机上通过醋酸铀酰水溶液、蒸馏水冲洗和铅染色对多达 20 个网格进行后切片染色的操作。TEM 结果与手动染色完全相同，但利用低成本的 HistoEnder、仪器和设备实现自动化更具优势。

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Adaptation of the HistoEnder, an open-source 3D printer for automated transmission electron microscopy grid staining.

The HistoEnder, an inexpensive open-source 3D printer published as an automated histological slide stainer, has been adapted for conventional biological transmission electron microscopy (TEM) batch grid staining. Details are presented of the 3D printed apparatus, assembly, G-code programming, and operation on the 3D printer to post-section stains up to 20 grids through aqueous uranyl acetate, distilled water rinses, and lead stains. TEM Results are identical to manual staining with the advantages of automation using the low cost HistoEnder, apparatus, and equipment.

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来源期刊

Journal of Histotechnology 生物-细胞生物学

CiteScore

2.60

自引率

9.10%

发文量

审稿时长

>12 weeks

期刊介绍： The official journal of the National Society for Histotechnology, Journal of Histotechnology, aims to advance the understanding of complex biological systems and improve patient care by applying histotechniques to diagnose, prevent and treat diseases. Journal of Histotechnology is concerned with educating practitioners and researchers from diverse disciplines about the methods used to prepare tissues and cell types, from all species, for microscopic examination. This is especially relevant to Histotechnicians. Journal of Histotechnology welcomes research addressing new, improved, or traditional techniques for tissue and cell preparation. This includes review articles, original articles, technical notes, case studies, advances in technology, and letters to editors. Topics may include, but are not limited to, discussion of clinical, veterinary, and research histopathology.