通过 TRAP 和 ERK 生物标记监测慢性高胆固醇血症雄性大鼠骨基质的酸化。

IF 0.9 Q3 VETERINARY SCIENCES Open Veterinary Journal Pub Date : 2024-08-01 Epub Date: 2024-08-31 DOI:10.5455/OVJ.2024.v14.i8.11
Duaa Raad Abd-Al-Ameer, Wefak Albazi, Hayder Ali Muhammed
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引用次数: 0

摘要

背景:高胆固醇血症经常与心血管疾病(包括心脏病发作和中风)的风险升高有关。此外,它还可能与骨质疏松症的高易感性有关。高胆固醇血症可刺激破骨细胞的分化和活性,导致骨吸收增强,进而造成骨组织的净损失。目的:本研究旨在探讨高胆固醇饮食对雄性大鼠骨质疏松症的影响,以及高胆固醇血症饮食对雄性大鼠生物和氧化指标的影响:本研究的样本为 20 只雄性大鼠,年龄在 1.5 个月到 2 个月之间,分为两组。一组中的 10 只大鼠摄入普通饮食,另一组中的 10 只大鼠摄入高胆固醇饮食(2%),为期 8 周。在实验的最后阶段采集血液样本。计算生理和生物指标,包括细胞外信号调节激酶(ERK)、耐酒石酸磷酸酶(TRAP)、激素、丙二醛(MDA)和谷胱甘肽(GSH):研究结果表明,胆固醇组的 GSH 水平降低,ERKs 水平升高,血清 TRAP 水平无明显变化,血液中 MDA 水平升高,甲状旁腺激素、降钙素和维生素 D 水平升高:结论:氧化应激增加、信号传导改变、钙/骨代谢紊乱与胆固醇相关疾病有关,监测生物标志物ERK可为疾病进展提供有价值的信息。
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Monitoring of bone matrix acidification by TRAP and ERK biomarkers in the chronic hypercholesterolemia male rats.

Background: Hypercholesterolemia is frequently linked to an elevated risk of cardiovascular diseases, including heart attacks and strokes. Additionally, it could be connected to a higher susceptibility to osteoporosis. Hypercholesterolemia can stimulate the differentiation and activity of osteoclasts, leading to enhanced bone reabsorption and a subsequent net loss of bone tissue.

Aim: The purpose of this study was to examine the influence of a high-cholesterol diet on osteoporosis in male rats with differences in biological and oxidative indicators in the hypercholesterolemia diet in male rats.

Methods: The samples in this study were twenty male rats, ranging between 1.5 and 2 months, were separated into two groups. In one group, 10 rats were fed a regular diet, while in another group, 10 rats were fed a high-cholesterol diet (2%) over the course of 8 weeks. Samples of blood were obtained at the last stage of the experiment. To calculate physiological and biological markers including extracellular signal-regulated kinase (ERK), tartrate-resistant acid phosphatase (TRAP), hormones, malondialdehyde (MDA), and glutathione (GSH).

Results: The results of this study demonstrated a decrease in GSH levels, an increase in ERKs, no significant change in serum TRAP levels, an increase in MDA levels in the blood, and elevated levels of parathyroid hormone, calcitonin, and vitamin D in the cholesterol group.

Conclusion: Increased oxidative stress, altered signaling, and disruptions in calcium/bone metabolism associated with cholesterol-related conditions and monitoring biomarker ERK can provide valuable information about disease progression.

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来源期刊
Open Veterinary Journal
Open Veterinary Journal VETERINARY SCIENCES-
CiteScore
1.40
自引率
0.00%
发文量
112
审稿时长
12 weeks
期刊介绍: Open Veterinary Journal is a peer-reviewed international open access online and printed journal that publishes high-quality original research articles. reviews, short communications and case reports dedicated to all aspects of veterinary sciences and its related subjects. Research areas include the following: Infectious diseases of zoonotic/food-borne importance, applied biochemistry, parasitology, endocrinology, microbiology, immunology, pathology, pharmacology, physiology, epidemiology, molecular biology, immunogenetics, surgery, ophthalmology, dermatology, oncology and animal reproduction. All papers are peer-reviewed. Moreover, with the presence of well-qualified group of international referees, the process of publication will be done meticulously and to the highest standards.
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