Lei Xia, Han Wang, Xiaokun Zhao, Qinzheng Zhao, Xiaqing Yu, Ji Li, Qunfeng Lou, Jinfeng Chen, Chunyan Cheng
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Cucumis spp. contains the species, i.e., cucumber and melon, which chloroplasts and mitochondria are maternally inherited and paternally inherited, respectively, serving as good materials for the study of reciprocal differences. In this study, leaf color asymmetry was observed in the reciprocal hybrids (HC and CH) derived from C. sativus (2n = 14, CC) and C. hystrix (2n = 24, HH), where the leaves of HC were found to have reduced chlorophyll content, abnormal chloroplast structure and lower photosynthetic capacity. Transcriptomic analysis revealed that the chloroplast development-related genes were differentially expressed in leaf color asymmetry. Genetic analysis showed that leaf color asymmetry was caused by the maternal chloroplast genome. Comparative analysis of chloroplast genomes revealed that there was no mutation in the chloroplast genome during interspecific hybridization. Moreover, a PPR gene (CsaV3_1G038250.1) with RNA-editing function was found to be involved in the regulation of leaf color asymmetry. 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引用次数: 0
摘要
主要结论在互交杂种 C. hystrix × C. sativus(HC)和 C. sativus × C. hystrix(CH)中发现的叶色不对称现象可能受 CsPPR 基因(CsaV3_1G038250.1)的影响。大多数被子植物的细胞器是母系遗传的;因此,互交杂种通常表现出与母本亲本相关的不对称表型。然而,植物细胞质中有两套细胞器基因组,互作差异的机理更为复杂,而且基本上是未知的,因为除了线粒体基因外,叶绿体基因也参与其中。黄瓜属植物中的黄瓜和甜瓜分别是叶绿体和线粒体母系遗传和父系遗传的物种,是研究互作差异的良好材料。本研究观察了由 C. sativus(2n = 14,CC)和 C. hystrix(2n = 24,HH)衍生的互交杂种(HC 和 CH)的叶色不对称现象,发现 HC 的叶片叶绿素含量降低,叶绿体结构异常,光合能力较低。转录组分析表明,叶绿体发育相关基因在叶色不对称中表达不同。遗传分析表明,叶色不对称是由母体叶绿体基因组引起的。叶绿体基因组的比较分析表明,在种间杂交过程中,叶绿体基因组没有发生突变。此外,还发现一个具有 RNA 编辑功能的 PPR 基因(CsaV3_1G038250.1)参与了叶色不对称的调控。这些发现为植物互交中不对称表型的调控机制提供了新的见解。
The CsPPR gene with RNA-editing function involved in leaf color asymmetry of the reciprocal hybrids derived from Cucumis sativus and C. hystrix.
Main conclusion: The leaf color asymmetry found in the reciprocal hybrids C. hystrix × C. sativus (HC) and C. sativus × C. hystrix (CH) could be influenced by the CsPPR gene (CsaV3_1G038250.1). Most angiosperm organelles are maternally inherited; thus, the reciprocal hybrids usually exhibit asymmetric phenotypes that are associated with the maternal parent. However, there are two sets of organelle genomes in the plant cytoplasm, and the mechanism of reciprocal differences are more complex and largely unknown, because the chloroplast genes are involved besides mitochondrial genes. Cucumis spp. contains the species, i.e., cucumber and melon, which chloroplasts and mitochondria are maternally inherited and paternally inherited, respectively, serving as good materials for the study of reciprocal differences. In this study, leaf color asymmetry was observed in the reciprocal hybrids (HC and CH) derived from C. sativus (2n = 14, CC) and C. hystrix (2n = 24, HH), where the leaves of HC were found to have reduced chlorophyll content, abnormal chloroplast structure and lower photosynthetic capacity. Transcriptomic analysis revealed that the chloroplast development-related genes were differentially expressed in leaf color asymmetry. Genetic analysis showed that leaf color asymmetry was caused by the maternal chloroplast genome. Comparative analysis of chloroplast genomes revealed that there was no mutation in the chloroplast genome during interspecific hybridization. Moreover, a PPR gene (CsaV3_1G038250.1) with RNA-editing function was found to be involved in the regulation of leaf color asymmetry. These findings provide new insights into the regulatory mechanisms of asymmetric phenotypes in plant reciprocal crosses.
期刊介绍:
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