聚合酶链式反应对皮肤利什曼病组织活检革兰氏染色的诊断准确性。

Aleena Khalid, Maria Mushtaq Gill, Irfan Ali Mirza, Sakeenah Hussain Naqvi, Syed Adeel Hussain Gardezi, Rabia Sajjad
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引用次数: 0

摘要

目的研究设计:横断面分析研究。研究地点和时间:巴基斯坦拉瓦尔品第武装部队病理研究所/国立医科大学微生物学系,2022 年 7 月至 12 月:对 98 名疑似 CL 患者的皮肤组织样本进行评估。方法:对 98 名疑似 CL 患者的皮肤组织样本进行评估,并对这些样本进行 Giemsa 染色显微镜检查和实时 PCR 检测。以革兰染色组织切片显微镜检查为金标准,计算 PCR 的灵敏度、特异性和准确性:结果:在 98 份组织样本中,37 份在 PCR 检测中发现利什曼病阳性,13 份在革兰染色组织切片显微镜检测中发现利什曼病阳性。PCR 检测利什曼原虫的敏感性、特异性和准确性分别为 100%、71.8% 和 91.8%,阴性预测值为 100%:本研究表明,商用 PCR 是诊断 CL 的可靠检测方法。PCR的简便、快速和可靠性使其成为诊断CL的可靠工具:革兰氏染色法 利什曼原虫 聚合酶链反应 Viasure
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Diagnostic Accuracy of Polymerase Chain Reaction Against Giemsa Staining on Tissue Biopsy for Cutaneous Leishmaniasis.

Objective: To evaluate the diagnostic accuracy of a commercial real-time polymerase chain reaction (PCR) kit targeting 18S rRNA against Giemsa-stained tissue slides in patients clinically suspected of cutaneous leishmaniasis (CL).

Study design: Cross-sectional analytical study. Place and Duration of the Study: Department of Microbiology, Armed Forces Institute of Pathology / National University of Medical Sciences, Rawalpindi, Pakistan, from July to December 2022.

Methodology: Samples of skin tissue in 98 patients suspected of CL were evaluated. These samples were subjected to Giemsa-staining for microscopy and real-time PCR. Sensitivity, specificity, and accuracy of the PCR were calculated keeping Giemsa-stained tissue slide microscopy as gold standard.

Results: Out of the 98 tissue samples, 37 were found positive for leishmaniasis on PCR while 13 were found Leishmania positive on microscopy of Giemsa-stained slides. The sensitivity, specificity, and accuracy of the PCR for the detection of Leishmania species were 100%, 71.8%, and 91.8%, respectively with 100% negative predictive value.

Conclusion: This study demonstrates that the commercial PCR is a reliable diagnostic test for the diagnosis of CL. The ease, rapidity, and reliability of the PCR make it a dependable tool in diagnostic repertoire of CL.

Key words: Giemsa stain, Leishmania spp., Polymerase chain reaction, Viasure.

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