{"title":"砷可减少稻田土壤的甲烷排放:大陆调查和实验室培育的启示。","authors":"Ou-Yuan Jiang, Si-Yu Zhang, Xin-Di Zhao, Zi-Teng Liu, Andreas Kappler, Jian-Ming Xu, Xian-Jin Tang","doi":"10.1021/acs.est.4c06809","DOIUrl":null,"url":null,"abstract":"<p><p>Arsenic (As) contamination and methane (CH<sub>4</sub>) emissions co-occur in rice paddies. However, how As impacts CH<sub>4</sub> production, oxidation, and emission dynamics is unknown. Here, we investigated the abundances and activities of CH<sub>4</sub>-cycling microbes from 132 paddy soils with different As concentrations across continental China using metagenomics and the reverse transcription polymerase chain reaction. Our results revealed that As was a crucial factor affecting the abundance and distribution patterns of the <i>mcrA</i> gene, which is responsible for CH<sub>4</sub> production and anaerobic CH<sub>4</sub> oxidation. Laboratory incubation experiments showed that adding 30 mg kg<sup>-1</sup> arsenate increased <sup>13</sup>CO<sub>2</sub> production by 10-fold, ultimately decreasing CH<sub>4</sub> emissions by 68.5%. The inhibition of CH<sub>4</sub> emissions by As was induced through three aspects: (1) the toxicity of As decreased the abundance and activity of the methanogens; (2) the adaptability and response of methanotrophs to As is beneficial for CH<sub>4</sub> oxidation under As stress; and (3) the more robust arsenate reduction would anaerobically consume more CH<sub>4</sub> in paddies. Additionally, significant positive correlations were observed between <i>arsC</i> and <i>pmoA</i> gene abundance in both the observational study and incubation experiment. These findings enhance our understanding of the mechanisms underlying the interactions between As and CH<sub>4</sub> cycling in soils.</p>","PeriodicalId":36,"journal":{"name":"环境科学与技术","volume":null,"pages":null},"PeriodicalIF":10.8000,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Arsenic Reduces Methane Emissions from Paddy Soils: Insights from Continental Investigation and Laboratory Incubations.\",\"authors\":\"Ou-Yuan Jiang, Si-Yu Zhang, Xin-Di Zhao, Zi-Teng Liu, Andreas Kappler, Jian-Ming Xu, Xian-Jin Tang\",\"doi\":\"10.1021/acs.est.4c06809\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Arsenic (As) contamination and methane (CH<sub>4</sub>) emissions co-occur in rice paddies. However, how As impacts CH<sub>4</sub> production, oxidation, and emission dynamics is unknown. Here, we investigated the abundances and activities of CH<sub>4</sub>-cycling microbes from 132 paddy soils with different As concentrations across continental China using metagenomics and the reverse transcription polymerase chain reaction. Our results revealed that As was a crucial factor affecting the abundance and distribution patterns of the <i>mcrA</i> gene, which is responsible for CH<sub>4</sub> production and anaerobic CH<sub>4</sub> oxidation. Laboratory incubation experiments showed that adding 30 mg kg<sup>-1</sup> arsenate increased <sup>13</sup>CO<sub>2</sub> production by 10-fold, ultimately decreasing CH<sub>4</sub> emissions by 68.5%. The inhibition of CH<sub>4</sub> emissions by As was induced through three aspects: (1) the toxicity of As decreased the abundance and activity of the methanogens; (2) the adaptability and response of methanotrophs to As is beneficial for CH<sub>4</sub> oxidation under As stress; and (3) the more robust arsenate reduction would anaerobically consume more CH<sub>4</sub> in paddies. Additionally, significant positive correlations were observed between <i>arsC</i> and <i>pmoA</i> gene abundance in both the observational study and incubation experiment. These findings enhance our understanding of the mechanisms underlying the interactions between As and CH<sub>4</sub> cycling in soils.</p>\",\"PeriodicalId\":36,\"journal\":{\"name\":\"环境科学与技术\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":10.8000,\"publicationDate\":\"2024-09-24\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"环境科学与技术\",\"FirstCategoryId\":\"1\",\"ListUrlMain\":\"https://doi.org/10.1021/acs.est.4c06809\",\"RegionNum\":1,\"RegionCategory\":\"环境科学与生态学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ENGINEERING, ENVIRONMENTAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"环境科学与技术","FirstCategoryId":"1","ListUrlMain":"https://doi.org/10.1021/acs.est.4c06809","RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENGINEERING, ENVIRONMENTAL","Score":null,"Total":0}
Arsenic Reduces Methane Emissions from Paddy Soils: Insights from Continental Investigation and Laboratory Incubations.
Arsenic (As) contamination and methane (CH4) emissions co-occur in rice paddies. However, how As impacts CH4 production, oxidation, and emission dynamics is unknown. Here, we investigated the abundances and activities of CH4-cycling microbes from 132 paddy soils with different As concentrations across continental China using metagenomics and the reverse transcription polymerase chain reaction. Our results revealed that As was a crucial factor affecting the abundance and distribution patterns of the mcrA gene, which is responsible for CH4 production and anaerobic CH4 oxidation. Laboratory incubation experiments showed that adding 30 mg kg-1 arsenate increased 13CO2 production by 10-fold, ultimately decreasing CH4 emissions by 68.5%. The inhibition of CH4 emissions by As was induced through three aspects: (1) the toxicity of As decreased the abundance and activity of the methanogens; (2) the adaptability and response of methanotrophs to As is beneficial for CH4 oxidation under As stress; and (3) the more robust arsenate reduction would anaerobically consume more CH4 in paddies. Additionally, significant positive correlations were observed between arsC and pmoA gene abundance in both the observational study and incubation experiment. These findings enhance our understanding of the mechanisms underlying the interactions between As and CH4 cycling in soils.
期刊介绍:
Environmental Science & Technology (ES&T) is a co-sponsored academic and technical magazine by the Hubei Provincial Environmental Protection Bureau and the Hubei Provincial Academy of Environmental Sciences.
Environmental Science & Technology (ES&T) holds the status of Chinese core journals, scientific papers source journals of China, Chinese Science Citation Database source journals, and Chinese Academic Journal Comprehensive Evaluation Database source journals. This publication focuses on the academic field of environmental protection, featuring articles related to environmental protection and technical advancements.