Michael J Neuhoff, Yuchen Wang, Nicholas J Vantangoli, Michael G Poirier, Carlos E Castro, Wolfgang G Pfeifer
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引用次数: 0
摘要
DNA 折纸纳米技术在生物医学、生物物理和纳米制造应用等多个领域具有巨大潜力。然而,目前的生产流水线只能生产出一次性使用的装置,其中只含有一小部分初始反应物,造成了生产过程的浪费。在这里,我们介绍了两种互补方法,通过回收 DNA 折纸纳米结构(DON)的链成分来克服这些限制。我们展示了通过重复使用支架链将整个 DONs 重新编程为新器件的方法。我们通过将具有复杂几何结构的 DONs 互相重新编程来验证这种方法,利用它们不同的几何结构来验证支架的成功再循环。我们将一种 DON 重编程为动态结构,结果显示原始结构和回收结构都显示出相似的特性。其次,我们展示了组装后多余主链的回收,并用这些回收的主链折叠 DON,显示出这些结构具有预期的几何形状和动态特性。最后,我们展示了这两种方法的结合,成功地利用回收的 DNA 成分制造出了 DONs。
Recycling Materials for Sustainable DNA Origami Manufacturing.
DNA origami nanotechnology has great potential in multiple fields including biomedical, biophysical, and nanofabrication applications. However, current production pipelines lead to single-use devices incorporating a small fraction of initial reactants, resulting in a wasteful manufacturing process. Here, we introduce two complementary approaches to overcome these limitations by recycling the strand components of DNA origami nanostructures (DONs). We demonstrate reprogramming entire DONs into new devices, reusing scaffold strands. We validate this approach by reprogramming DONs with complex geometries into each other, using their distinct geometries to verify successful scaffold recycling. We reprogram one DON into a dynamic structure and show both pristine and recycled structures display similar properties. Second, we demonstrate the recovery of excess staple strands postassembly and fold DONs with these recycled strands, showing these structures exhibit the expected geometry and dynamic properties. Finally, we demonstrate the combination of both approaches, successfully fabricating DONs solely from recycled DNA components.
期刊介绍:
Nano Letters serves as a dynamic platform for promptly disseminating original results in fundamental, applied, and emerging research across all facets of nanoscience and nanotechnology. A pivotal criterion for inclusion within Nano Letters is the convergence of at least two different areas or disciplines, ensuring a rich interdisciplinary scope. The journal is dedicated to fostering exploration in diverse areas, including:
- Experimental and theoretical findings on physical, chemical, and biological phenomena at the nanoscale
- Synthesis, characterization, and processing of organic, inorganic, polymer, and hybrid nanomaterials through physical, chemical, and biological methodologies
- Modeling and simulation of synthetic, assembly, and interaction processes
- Realization of integrated nanostructures and nano-engineered devices exhibiting advanced performance
- Applications of nanoscale materials in living and environmental systems
Nano Letters is committed to advancing and showcasing groundbreaking research that intersects various domains, fostering innovation and collaboration in the ever-evolving field of nanoscience and nanotechnology.