Ying Li, Wendi Wang, Zijian Liu, Guangjing Liu, Xiaobing Li
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引用次数: 0
摘要
整合素α（ITGA）亚家族基因在多种癌症中发挥着重要作用。然而，ITGA基因在人脂肪源性干细胞（hADSCs）成脂分化中的潜在机制和应用价值仍不明确。本研究证实，ITGA2/3/5 mRNA表达在脂肪生成过程中受到抑制。阻断ITGA2/3/5可促进hADSCs的成脂分化。油红 O 染色发现，在诱导成脂 14 d 后，ITGA2/3/5 抑制组比对照组有更多的脂滴。此外，抑制 ITGA2/3/5 可促进脂肪生成相关基因（PPAR-γ、C/EBPα、FABP4）的表达。从机理上讲，ITGA2/3/5是通过调节Rac1信号通路发挥作用的，这合理地解释了ITGA2/3/5在hADSCs成脂分化中的作用。我们的研究表明，阻断ITGA2/3/5可促进hADSCs的成脂分化。
Blockade of ITGA2/3/5 Promotes Adipogenic Differentiation of Human Adipose-derived Mesenchymal Stem Cells.
The integrin α (ITGA) subfamily genes play a fundamental role in various cancers. However, the potential mechanism and application values of ITGA genes in adipogenic differentiation of human adipose-derived stem cells (hADSCs) remain elusive. This study confirmed that ITGA2/3/5 mRNA expressions were repressed during adipogenesis. Blockade of ITGA2/3/5 enhanced adipogenic differentiation of hADSCs. Oil red O staining found that more lipid droplets were apparent in the ITGA2/3/5 inhibition group following 14 d adipogenic induction than in the control group. In addition, inhibition of ITGA2/3/5 promoted the expression of adipogenesis-related genes (PPAR-γ, C/EBPα, FABP4). Mechanistically, ITGA2/3/5 functioned by regulating the Rac1 signaling pathway, which reasonably explains ITGA2/3/5's role in adipogenic differentiation of hADSCs. Our studies suggest that blockades of ITGA2/3/5 promote the adipogenic differentiation of hADSCs.
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Cell Biochemistry and Biophysics (CBB) aims to publish papers on the nature of the biochemical and biophysical mechanisms underlying the structure, control and function of cellular systems
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