Merat Karimi, Ehsan Sadeghi, Mostafa Zahedifar, Hamed Mirzaei, Majid Nejati, Michael R Hamblin
{"title":"利用柚木提取物绿色合成掺金氧化锡纳米粒子,在光动力疗法中的潜在应用","authors":"Merat Karimi, Ehsan Sadeghi, Mostafa Zahedifar, Hamed Mirzaei, Majid Nejati, Michael R Hamblin","doi":"10.1089/photob.2024.0052","DOIUrl":null,"url":null,"abstract":"<p><p><b><i>Objective:</i></b> The green synthesis of Tin(IV) oxide (SnO<sub>2</sub>): Gold (Au) nanoparticles (NPs) using <i>Teucrium polium</i> medicinal plant extract was investigated, and the NPs were characterized and tested as photosensitizers to produce reactive oxygen species (ROS). <b><i>Methods:</i></b> The cytotoxic effect on C26 cells was investigated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) technique. The results showed their toxicity in a dose-dependent manner. The green synthesis of SnO<sub>2</sub>:Au NPs was achieved for the first time using an extract of <i>T. polium</i> medicinal plant as a reducing and stabilizing agent. The produced NPs were examined for their application in photodynamic therapy (PDT) for cancer. <b><i>Results:</i></b> Methylene blue and anthracene were used to confirm that the photosensitizer could produce ROS when excited with UVA radiation. The anticancer activity of SnO<sub>2</sub>:Au was investigated in vitro using the C26 cell line and an MTT assay, showing that PDT with SnO<sub>2</sub>:Au NPs could inhibit cancer cell proliferation. <b><i>Conclusions:</i></b> The significant afterglow of the SnO<sub>2</sub>:Au NPs could cause the generation of ROS to continue several minutes after switching off the light source.</p>","PeriodicalId":94169,"journal":{"name":"Photobiomodulation, photomedicine, and laser surgery","volume":" ","pages":"643-652"},"PeriodicalIF":1.8000,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Green Synthesis of Au-Doped Tin Oxide Nanoparticles Using <i>Teucrium Polium</i> Extract with Potential Applications in Photodynamic Therapy.\",\"authors\":\"Merat Karimi, Ehsan Sadeghi, Mostafa Zahedifar, Hamed Mirzaei, Majid Nejati, Michael R Hamblin\",\"doi\":\"10.1089/photob.2024.0052\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p><b><i>Objective:</i></b> The green synthesis of Tin(IV) oxide (SnO<sub>2</sub>): Gold (Au) nanoparticles (NPs) using <i>Teucrium polium</i> medicinal plant extract was investigated, and the NPs were characterized and tested as photosensitizers to produce reactive oxygen species (ROS). <b><i>Methods:</i></b> The cytotoxic effect on C26 cells was investigated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) technique. The results showed their toxicity in a dose-dependent manner. The green synthesis of SnO<sub>2</sub>:Au NPs was achieved for the first time using an extract of <i>T. polium</i> medicinal plant as a reducing and stabilizing agent. The produced NPs were examined for their application in photodynamic therapy (PDT) for cancer. <b><i>Results:</i></b> Methylene blue and anthracene were used to confirm that the photosensitizer could produce ROS when excited with UVA radiation. The anticancer activity of SnO<sub>2</sub>:Au was investigated in vitro using the C26 cell line and an MTT assay, showing that PDT with SnO<sub>2</sub>:Au NPs could inhibit cancer cell proliferation. <b><i>Conclusions:</i></b> The significant afterglow of the SnO<sub>2</sub>:Au NPs could cause the generation of ROS to continue several minutes after switching off the light source.</p>\",\"PeriodicalId\":94169,\"journal\":{\"name\":\"Photobiomodulation, photomedicine, and laser surgery\",\"volume\":\" \",\"pages\":\"643-652\"},\"PeriodicalIF\":1.8000,\"publicationDate\":\"2024-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Photobiomodulation, photomedicine, and laser surgery\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1089/photob.2024.0052\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/9/24 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q2\",\"JCRName\":\"SURGERY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Photobiomodulation, photomedicine, and laser surgery","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1089/photob.2024.0052","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/9/24 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"SURGERY","Score":null,"Total":0}
Green Synthesis of Au-Doped Tin Oxide Nanoparticles Using Teucrium Polium Extract with Potential Applications in Photodynamic Therapy.
Objective: The green synthesis of Tin(IV) oxide (SnO2): Gold (Au) nanoparticles (NPs) using Teucrium polium medicinal plant extract was investigated, and the NPs were characterized and tested as photosensitizers to produce reactive oxygen species (ROS). Methods: The cytotoxic effect on C26 cells was investigated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) technique. The results showed their toxicity in a dose-dependent manner. The green synthesis of SnO2:Au NPs was achieved for the first time using an extract of T. polium medicinal plant as a reducing and stabilizing agent. The produced NPs were examined for their application in photodynamic therapy (PDT) for cancer. Results: Methylene blue and anthracene were used to confirm that the photosensitizer could produce ROS when excited with UVA radiation. The anticancer activity of SnO2:Au was investigated in vitro using the C26 cell line and an MTT assay, showing that PDT with SnO2:Au NPs could inhibit cancer cell proliferation. Conclusions: The significant afterglow of the SnO2:Au NPs could cause the generation of ROS to continue several minutes after switching off the light source.