Manokari M., Mohammad Faisal, Abdulrahman A. Alatar, Mahipal S Shekhawat
{"title":"揭示纳米硅粒子对 Cadaba trifoliata (Roxb.) Wight & Arn 直接再生和微结构发展的刺激作用","authors":"Manokari M., Mohammad Faisal, Abdulrahman A. Alatar, Mahipal S Shekhawat","doi":"10.1039/d4en00640b","DOIUrl":null,"url":null,"abstract":"Nanosilicon positively affects the growth and development of plants. Investigations were carried out to analyse the effect of applied silicon nanoparticles (SiNPs) on the multiplication and rooting of the shoots of Cadaba trifoliata (Capparaceae). In vitro-raised shoots were cultivated on Murashige and Skoog (MS) medium with 0.5 mg L-1 meta Topolin/mT and 0.25 mg L-1 indole-3 acetic acid (IAA) along with various concentrations of SiNPs. 2.0 mg L-1 nanosilicon along with optimal plant hormones improved multiplication of shoot numbers and morphology (25 shoots and 13.0 leaves shoot-1), leaf area (1.3 × 0.9 cm), biomass (647 mg fresh weight; 114 mg dry weight) production, and photopigments (Chl a: 378.0, Chl b: 513.0, and Cx+ c: 60.0 μg g-1 fresh weight). Cent percent of the shoots were rooted in half-strength MS medium with 1.25 mg L-1 indole-3-butyric acid (IBA). Incorporation of 2.0 mg L-1 SiNPs in medium promoted rooting (8.3 roots per shoot, each 4.93 cm long). Analyses of qualitative microscopic data revealed that the leaves developed from the cloned shoots exhibited well-organized stomatal guard cells and epidermal cells, thick cuticle and epidermis, and increased tissue density in mesophyll and vascular tissues. The stem and root anatomy revealed improvements in vascular tissue density and lignification in xylem vessels and phloem elements. The plantlets were acclimatized under ex vitro conditions for 8 weeks in soilrite and vermicompost mixture. These findings highlight the potential of the SiNPs in improving regeneration potential for clonal propagation of C. trifoliata.","PeriodicalId":73,"journal":{"name":"Environmental Science: Nano","volume":null,"pages":null},"PeriodicalIF":5.8000,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Unveiling the stimulatory effect of Silicon nanoparticles on direct regeneration and micro-structural developments in Cadaba trifoliata (Roxb.) Wight & Arn\",\"authors\":\"Manokari M., Mohammad Faisal, Abdulrahman A. Alatar, Mahipal S Shekhawat\",\"doi\":\"10.1039/d4en00640b\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Nanosilicon positively affects the growth and development of plants. Investigations were carried out to analyse the effect of applied silicon nanoparticles (SiNPs) on the multiplication and rooting of the shoots of Cadaba trifoliata (Capparaceae). In vitro-raised shoots were cultivated on Murashige and Skoog (MS) medium with 0.5 mg L-1 meta Topolin/mT and 0.25 mg L-1 indole-3 acetic acid (IAA) along with various concentrations of SiNPs. 2.0 mg L-1 nanosilicon along with optimal plant hormones improved multiplication of shoot numbers and morphology (25 shoots and 13.0 leaves shoot-1), leaf area (1.3 × 0.9 cm), biomass (647 mg fresh weight; 114 mg dry weight) production, and photopigments (Chl a: 378.0, Chl b: 513.0, and Cx+ c: 60.0 μg g-1 fresh weight). Cent percent of the shoots were rooted in half-strength MS medium with 1.25 mg L-1 indole-3-butyric acid (IBA). Incorporation of 2.0 mg L-1 SiNPs in medium promoted rooting (8.3 roots per shoot, each 4.93 cm long). Analyses of qualitative microscopic data revealed that the leaves developed from the cloned shoots exhibited well-organized stomatal guard cells and epidermal cells, thick cuticle and epidermis, and increased tissue density in mesophyll and vascular tissues. The stem and root anatomy revealed improvements in vascular tissue density and lignification in xylem vessels and phloem elements. The plantlets were acclimatized under ex vitro conditions for 8 weeks in soilrite and vermicompost mixture. These findings highlight the potential of the SiNPs in improving regeneration potential for clonal propagation of C. trifoliata.\",\"PeriodicalId\":73,\"journal\":{\"name\":\"Environmental Science: Nano\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":5.8000,\"publicationDate\":\"2024-09-24\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Environmental Science: Nano\",\"FirstCategoryId\":\"6\",\"ListUrlMain\":\"https://doi.org/10.1039/d4en00640b\",\"RegionNum\":2,\"RegionCategory\":\"环境科学与生态学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Environmental Science: Nano","FirstCategoryId":"6","ListUrlMain":"https://doi.org/10.1039/d4en00640b","RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
Unveiling the stimulatory effect of Silicon nanoparticles on direct regeneration and micro-structural developments in Cadaba trifoliata (Roxb.) Wight & Arn
Nanosilicon positively affects the growth and development of plants. Investigations were carried out to analyse the effect of applied silicon nanoparticles (SiNPs) on the multiplication and rooting of the shoots of Cadaba trifoliata (Capparaceae). In vitro-raised shoots were cultivated on Murashige and Skoog (MS) medium with 0.5 mg L-1 meta Topolin/mT and 0.25 mg L-1 indole-3 acetic acid (IAA) along with various concentrations of SiNPs. 2.0 mg L-1 nanosilicon along with optimal plant hormones improved multiplication of shoot numbers and morphology (25 shoots and 13.0 leaves shoot-1), leaf area (1.3 × 0.9 cm), biomass (647 mg fresh weight; 114 mg dry weight) production, and photopigments (Chl a: 378.0, Chl b: 513.0, and Cx+ c: 60.0 μg g-1 fresh weight). Cent percent of the shoots were rooted in half-strength MS medium with 1.25 mg L-1 indole-3-butyric acid (IBA). Incorporation of 2.0 mg L-1 SiNPs in medium promoted rooting (8.3 roots per shoot, each 4.93 cm long). Analyses of qualitative microscopic data revealed that the leaves developed from the cloned shoots exhibited well-organized stomatal guard cells and epidermal cells, thick cuticle and epidermis, and increased tissue density in mesophyll and vascular tissues. The stem and root anatomy revealed improvements in vascular tissue density and lignification in xylem vessels and phloem elements. The plantlets were acclimatized under ex vitro conditions for 8 weeks in soilrite and vermicompost mixture. These findings highlight the potential of the SiNPs in improving regeneration potential for clonal propagation of C. trifoliata.
期刊介绍:
Environmental Science: Nano serves as a comprehensive and high-impact peer-reviewed source of information on the design and demonstration of engineered nanomaterials for environment-based applications. It also covers the interactions between engineered, natural, and incidental nanomaterials with biological and environmental systems. This scope includes, but is not limited to, the following topic areas:
Novel nanomaterial-based applications for water, air, soil, food, and energy sustainability
Nanomaterial interactions with biological systems and nanotoxicology
Environmental fate, reactivity, and transformations of nanoscale materials
Nanoscale processes in the environment
Sustainable nanotechnology including rational nanomaterial design, life cycle assessment, risk/benefit analysis