Production of biogas from various types of spent mushroom substrate under different growth conditions

The mushroom cultivation industry effectively utilizes a diverse range of lignocellulosic waste but generates a significant amount of spent mushroom substrate (SMS) that poses disposal challenges. However, SMS can be used to generate valuable biogas. This study aimed to generate biogas by utilizing different types of spent mushroom substrate (SMS) obtained from three mushroom varieties. These mushroom varieties were cultivated in three types of greenhouses: Photovoltaic Shading greenhouse (PSG), shading greenhouse (SHG), and Control greenhouse (CG). The study found that the total solids (TS) and the amount of SMS used had a significant impact on both the total gas production and the removal of volatile solids (VS). The results demonstrated the feasibility of utilizing SMS as a raw material for biogas production, with variations observed depending on SMS type and greenhouse conditions. It contributes to knowledge in sustainable waste management and renewable energy production from agricultural by-products.